Potential pitfalls and solutions for use of fluorescent fusion proteins to study the lysosome.

Use of fusion protein tags to investigate lysosomal proteins can be complicated by the acidic, protease-rich environment of the lysosome. Potential artifacts include degradation or release of the tag and acid quenching of fluorescence. Tagging can also affect protein folding, glycosylation and/or tr...

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Bibliographic Details
Main Authors: Ling Huang, Douglas Pike, David E Sleat, Vikas Nanda, Peter Lobel
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3931630?pdf=render