Two different synchronous spectrofluorimetric approaches for simultaneous determination of febuxostat and ibuprofen
Two green, simple and sensitive synchronous spectrofluorimetric methods were developed for the first time for the simultaneous estimation of febuxostat (FEB) and ibuprofen (IBU). Method I is constant-wavelength synchronous spectrofluorimetry where FEB and IBU were recorded at 329 and 258 nm, respect...
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doaj-8745661899f341198b761dd4e40acfed2021-06-10T08:57:27ZengThe Royal SocietyRoyal Society Open Science2054-57032021-05-018510.1098/rsos.210354Two different synchronous spectrofluorimetric approaches for simultaneous determination of febuxostat and ibuprofenGalal Magdy0Fathalla Belal1Ahmed M. Abdel-Megied2Ahmed F. Abdel Hakiem3Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Kafrelsheikh University, P.O. Box 33511, Kafrelsheikh, EgyptPharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Mansoura University, P.O. Box 35516, Mansoura, EgyptPharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Kafrelsheikh University, P.O. Box 33511, Kafrelsheikh, EgyptPharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Kafrelsheikh University, P.O. Box 33511, Kafrelsheikh, EgyptTwo green, simple and sensitive synchronous spectrofluorimetric methods were developed for the first time for the simultaneous estimation of febuxostat (FEB) and ibuprofen (IBU). Method I is constant-wavelength synchronous spectrofluorimetry where FEB and IBU were recorded at 329 and 258 nm, respectively, using Δλ of 40 nm. Method II is constant-energy synchronous spectrofluorimetry using a wavenumber interval of −4000 cm−1. All measurements were carried out in a borate buffer of pH 7 and distilled water for dilution which increased the methods' greenness. The two methods were rectilinear over concentration ranges of 30.0–700.0 ng ml−1 and 0.5–9.0 µg ml−1 in the first method and 20.0–500.0 ng ml−1 and 0.1–8.0 µg ml−1 in the second method for FEB and IBU, respectively. High sensitivity was attained for the two drugs with limits of quantitations (LODs) down to 0.41 and 5.51 ng ml−1 in the first method and 0.25 and 3.32 ng ml−1 in the second method for FEB and IBU, respectively. Recovery percentages were in the range of 97.3–101.9% after extraction from spiked human plasma samples, demonstrating high bioanalytical applicability. The two methods were further applied to tablet dosage forms with good recovery results. The methods' greenness was assessed according to the analytical Eco-Scale and Green Analytical Procedure Index guidelines.https://royalsocietypublishing.org/doi/10.1098/rsos.210354synchronous spectrofluorimetryfebuxostatibuprofenhuman plasmagreen chemistry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Galal Magdy Fathalla Belal Ahmed M. Abdel-Megied Ahmed F. Abdel Hakiem |
spellingShingle |
Galal Magdy Fathalla Belal Ahmed M. Abdel-Megied Ahmed F. Abdel Hakiem Two different synchronous spectrofluorimetric approaches for simultaneous determination of febuxostat and ibuprofen Royal Society Open Science synchronous spectrofluorimetry febuxostat ibuprofen human plasma green chemistry |
author_facet |
Galal Magdy Fathalla Belal Ahmed M. Abdel-Megied Ahmed F. Abdel Hakiem |
author_sort |
Galal Magdy |
title |
Two different synchronous spectrofluorimetric approaches for simultaneous determination of febuxostat and ibuprofen |
title_short |
Two different synchronous spectrofluorimetric approaches for simultaneous determination of febuxostat and ibuprofen |
title_full |
Two different synchronous spectrofluorimetric approaches for simultaneous determination of febuxostat and ibuprofen |
title_fullStr |
Two different synchronous spectrofluorimetric approaches for simultaneous determination of febuxostat and ibuprofen |
title_full_unstemmed |
Two different synchronous spectrofluorimetric approaches for simultaneous determination of febuxostat and ibuprofen |
title_sort |
two different synchronous spectrofluorimetric approaches for simultaneous determination of febuxostat and ibuprofen |
publisher |
The Royal Society |
series |
Royal Society Open Science |
issn |
2054-5703 |
publishDate |
2021-05-01 |
description |
Two green, simple and sensitive synchronous spectrofluorimetric methods were developed for the first time for the simultaneous estimation of febuxostat (FEB) and ibuprofen (IBU). Method I is constant-wavelength synchronous spectrofluorimetry where FEB and IBU were recorded at 329 and 258 nm, respectively, using Δλ of 40 nm. Method II is constant-energy synchronous spectrofluorimetry using a wavenumber interval of −4000 cm−1. All measurements were carried out in a borate buffer of pH 7 and distilled water for dilution which increased the methods' greenness. The two methods were rectilinear over concentration ranges of 30.0–700.0 ng ml−1 and 0.5–9.0 µg ml−1 in the first method and 20.0–500.0 ng ml−1 and 0.1–8.0 µg ml−1 in the second method for FEB and IBU, respectively. High sensitivity was attained for the two drugs with limits of quantitations (LODs) down to 0.41 and 5.51 ng ml−1 in the first method and 0.25 and 3.32 ng ml−1 in the second method for FEB and IBU, respectively. Recovery percentages were in the range of 97.3–101.9% after extraction from spiked human plasma samples, demonstrating high bioanalytical applicability. The two methods were further applied to tablet dosage forms with good recovery results. The methods' greenness was assessed according to the analytical Eco-Scale and Green Analytical Procedure Index guidelines. |
topic |
synchronous spectrofluorimetry febuxostat ibuprofen human plasma green chemistry |
url |
https://royalsocietypublishing.org/doi/10.1098/rsos.210354 |
work_keys_str_mv |
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