Effect of initial pH of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by Rhodotorula glutinis
Background: Rhodotorula glutinis is capable of synthesizing numerous valuable metabolites with extensive potential industrial usage. This paper reports the effect of initial culture medium pH on growth and protein, lipid, and carotenoid biosynthesis by R. glutinis. Results: The highest biomass yield...
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doaj-892b0940ed1347d4a5ce60d8245d78d12020-11-24T20:47:15ZengElsevierElectronic Journal of Biotechnology0717-34582017-05-0127C253110.1016/j.ejbt.2017.01.007Effect of initial pH of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by Rhodotorula glutinisAnna M. Kot0Stanisław Błażejak1Agnieszka Kurcz2Joanna Bryś3Iwona Gientka4Anna Bzducha-Wróbel5Magdalena Maliszewska6Lidia Reczek7Department of Biotechnology, Microbiology and Food Evaluation, Faculty of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska 159C, 02-776 Warsaw, PolandDepartment of Biotechnology, Microbiology and Food Evaluation, Faculty of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska 159C, 02-776 Warsaw, PolandDepartment of Biotechnology, Microbiology and Food Evaluation, Faculty of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska 159C, 02-776 Warsaw, PolandDepartment of Chemistry, Faculty of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska 159C, 02-776 Warsaw, PolandDepartment of Biotechnology, Microbiology and Food Evaluation, Faculty of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska 159C, 02-776 Warsaw, PolandDepartment of Biotechnology, Microbiology and Food Evaluation, Faculty of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska 159C, 02-776 Warsaw, PolandDepartment of Biotechnology, Microbiology and Food Evaluation, Faculty of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska 159C, 02-776 Warsaw, PolandDepartment of Civil Engineering, Faculty of Civil and Environmental Engineering, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, PolandBackground: Rhodotorula glutinis is capable of synthesizing numerous valuable metabolites with extensive potential industrial usage. This paper reports the effect of initial culture medium pH on growth and protein, lipid, and carotenoid biosynthesis by R. glutinis. Results: The highest biomass yield was obtained in media with pH 4.0–7.0, and the value after 72 h was 17.2–19.4 gd.w./L. An initial pH of the medium in the range of 4.0–7.0 has no significant effect on the protein (38.5–41.3 g/100 gd.w.), lipid (10.2–12.7 g/100 gd.w.), or carotenoid (191.7–202.9 μg/gd.w.) content in the biomass or on the profile of synthesized fatty acids and carotenoids. The whole pool of fatty acids was dominated by oleic (48.1–53.4%), linoleic (21.4–25.1%), and palmitic acids (13.0–15.8%). In these conditions, the yeast mainly synthesized torulene (43.5–47.7%) and β-carotene (34.7–38.6%), whereas the contribution of torularhodin was only 12.1–16.8%. Cultivation in medium with initial pH 3.0 resulted in a reduction in growth (13.0 gd.w./L) and total carotenoid (115.8 μg/gd.w.), linoleic acid (11.5%), and torularhodin (4.5%) biosynthesis. Conclusion: The different values of initial pH of the culture medium with glycerol and deproteinized potato wastewater had a significant effect on the growth and protein, lipid, and carotenoid biosynthesis by R. glutinis.http://www.sciencedirect.com/science/article/pii/S0717345817300076CarotenoidsSingle cell oilSingle cell proteinWastesMedium pH |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anna M. Kot Stanisław Błażejak Agnieszka Kurcz Joanna Bryś Iwona Gientka Anna Bzducha-Wróbel Magdalena Maliszewska Lidia Reczek |
spellingShingle |
Anna M. Kot Stanisław Błażejak Agnieszka Kurcz Joanna Bryś Iwona Gientka Anna Bzducha-Wróbel Magdalena Maliszewska Lidia Reczek Effect of initial pH of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by Rhodotorula glutinis Electronic Journal of Biotechnology Carotenoids Single cell oil Single cell protein Wastes Medium pH |
author_facet |
Anna M. Kot Stanisław Błażejak Agnieszka Kurcz Joanna Bryś Iwona Gientka Anna Bzducha-Wróbel Magdalena Maliszewska Lidia Reczek |
author_sort |
Anna M. Kot |
title |
Effect of initial pH of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by Rhodotorula glutinis |
title_short |
Effect of initial pH of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by Rhodotorula glutinis |
title_full |
Effect of initial pH of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by Rhodotorula glutinis |
title_fullStr |
Effect of initial pH of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by Rhodotorula glutinis |
title_full_unstemmed |
Effect of initial pH of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by Rhodotorula glutinis |
title_sort |
effect of initial ph of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by rhodotorula glutinis |
publisher |
Elsevier |
series |
Electronic Journal of Biotechnology |
issn |
0717-3458 |
publishDate |
2017-05-01 |
description |
Background: Rhodotorula glutinis is capable of synthesizing numerous valuable metabolites with extensive potential industrial usage. This paper reports the effect of initial culture medium pH on growth and protein, lipid, and carotenoid biosynthesis by R. glutinis.
Results: The highest biomass yield was obtained in media with pH 4.0–7.0, and the value after 72 h was 17.2–19.4 gd.w./L. An initial pH of the medium in the range of 4.0–7.0 has no significant effect on the protein (38.5–41.3 g/100 gd.w.), lipid (10.2–12.7 g/100 gd.w.), or carotenoid (191.7–202.9 μg/gd.w.) content in the biomass or on the profile of synthesized fatty acids and carotenoids. The whole pool of fatty acids was dominated by oleic (48.1–53.4%), linoleic (21.4–25.1%), and palmitic acids (13.0–15.8%). In these conditions, the yeast mainly synthesized torulene (43.5–47.7%) and β-carotene (34.7–38.6%), whereas the contribution of torularhodin was only 12.1–16.8%. Cultivation in medium with initial pH 3.0 resulted in a reduction in growth (13.0 gd.w./L) and total carotenoid (115.8 μg/gd.w.), linoleic acid (11.5%), and torularhodin (4.5%) biosynthesis.
Conclusion: The different values of initial pH of the culture medium with glycerol and deproteinized potato wastewater had a significant effect on the growth and protein, lipid, and carotenoid biosynthesis by R. glutinis. |
topic |
Carotenoids Single cell oil Single cell protein Wastes Medium pH |
url |
http://www.sciencedirect.com/science/article/pii/S0717345817300076 |
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