Effect of initial pH of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by Rhodotorula glutinis

Background: Rhodotorula glutinis is capable of synthesizing numerous valuable metabolites with extensive potential industrial usage. This paper reports the effect of initial culture medium pH on growth and protein, lipid, and carotenoid biosynthesis by R. glutinis. Results: The highest biomass yield...

Full description

Bibliographic Details
Main Authors: Anna M. Kot, Stanisław Błażejak, Agnieszka Kurcz, Joanna Bryś, Iwona Gientka, Anna Bzducha-Wróbel, Magdalena Maliszewska, Lidia Reczek
Format: Article
Language:English
Published: Elsevier 2017-05-01
Series:Electronic Journal of Biotechnology
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0717345817300076
id doaj-892b0940ed1347d4a5ce60d8245d78d1
record_format Article
spelling doaj-892b0940ed1347d4a5ce60d8245d78d12020-11-24T20:47:15ZengElsevierElectronic Journal of Biotechnology0717-34582017-05-0127C253110.1016/j.ejbt.2017.01.007Effect of initial pH of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by Rhodotorula glutinisAnna M. Kot0Stanisław Błażejak1Agnieszka Kurcz2Joanna Bryś3Iwona Gientka4Anna Bzducha-Wróbel5Magdalena Maliszewska6Lidia Reczek7Department of Biotechnology, Microbiology and Food Evaluation, Faculty of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska 159C, 02-776 Warsaw, PolandDepartment of Biotechnology, Microbiology and Food Evaluation, Faculty of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska 159C, 02-776 Warsaw, PolandDepartment of Biotechnology, Microbiology and Food Evaluation, Faculty of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska 159C, 02-776 Warsaw, PolandDepartment of Chemistry, Faculty of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska 159C, 02-776 Warsaw, PolandDepartment of Biotechnology, Microbiology and Food Evaluation, Faculty of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska 159C, 02-776 Warsaw, PolandDepartment of Biotechnology, Microbiology and Food Evaluation, Faculty of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska 159C, 02-776 Warsaw, PolandDepartment of Biotechnology, Microbiology and Food Evaluation, Faculty of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska 159C, 02-776 Warsaw, PolandDepartment of Civil Engineering, Faculty of Civil and Environmental Engineering, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, PolandBackground: Rhodotorula glutinis is capable of synthesizing numerous valuable metabolites with extensive potential industrial usage. This paper reports the effect of initial culture medium pH on growth and protein, lipid, and carotenoid biosynthesis by R. glutinis. Results: The highest biomass yield was obtained in media with pH 4.0–7.0, and the value after 72 h was 17.2–19.4 gd.w./L. An initial pH of the medium in the range of 4.0–7.0 has no significant effect on the protein (38.5–41.3 g/100 gd.w.), lipid (10.2–12.7 g/100 gd.w.), or carotenoid (191.7–202.9 μg/gd.w.) content in the biomass or on the profile of synthesized fatty acids and carotenoids. The whole pool of fatty acids was dominated by oleic (48.1–53.4%), linoleic (21.4–25.1%), and palmitic acids (13.0–15.8%). In these conditions, the yeast mainly synthesized torulene (43.5–47.7%) and β-carotene (34.7–38.6%), whereas the contribution of torularhodin was only 12.1–16.8%. Cultivation in medium with initial pH 3.0 resulted in a reduction in growth (13.0 gd.w./L) and total carotenoid (115.8 μg/gd.w.), linoleic acid (11.5%), and torularhodin (4.5%) biosynthesis. Conclusion: The different values of initial pH of the culture medium with glycerol and deproteinized potato wastewater had a significant effect on the growth and protein, lipid, and carotenoid biosynthesis by R. glutinis.http://www.sciencedirect.com/science/article/pii/S0717345817300076CarotenoidsSingle cell oilSingle cell proteinWastesMedium pH
collection DOAJ
language English
format Article
sources DOAJ
author Anna M. Kot
Stanisław Błażejak
Agnieszka Kurcz
Joanna Bryś
Iwona Gientka
Anna Bzducha-Wróbel
Magdalena Maliszewska
Lidia Reczek
spellingShingle Anna M. Kot
Stanisław Błażejak
Agnieszka Kurcz
Joanna Bryś
Iwona Gientka
Anna Bzducha-Wróbel
Magdalena Maliszewska
Lidia Reczek
Effect of initial pH of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by Rhodotorula glutinis
Electronic Journal of Biotechnology
Carotenoids
Single cell oil
Single cell protein
Wastes
Medium pH
author_facet Anna M. Kot
Stanisław Błażejak
Agnieszka Kurcz
Joanna Bryś
Iwona Gientka
Anna Bzducha-Wróbel
Magdalena Maliszewska
Lidia Reczek
author_sort Anna M. Kot
title Effect of initial pH of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by Rhodotorula glutinis
title_short Effect of initial pH of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by Rhodotorula glutinis
title_full Effect of initial pH of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by Rhodotorula glutinis
title_fullStr Effect of initial pH of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by Rhodotorula glutinis
title_full_unstemmed Effect of initial pH of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by Rhodotorula glutinis
title_sort effect of initial ph of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by rhodotorula glutinis
publisher Elsevier
series Electronic Journal of Biotechnology
issn 0717-3458
publishDate 2017-05-01
description Background: Rhodotorula glutinis is capable of synthesizing numerous valuable metabolites with extensive potential industrial usage. This paper reports the effect of initial culture medium pH on growth and protein, lipid, and carotenoid biosynthesis by R. glutinis. Results: The highest biomass yield was obtained in media with pH 4.0–7.0, and the value after 72 h was 17.2–19.4 gd.w./L. An initial pH of the medium in the range of 4.0–7.0 has no significant effect on the protein (38.5–41.3 g/100 gd.w.), lipid (10.2–12.7 g/100 gd.w.), or carotenoid (191.7–202.9 μg/gd.w.) content in the biomass or on the profile of synthesized fatty acids and carotenoids. The whole pool of fatty acids was dominated by oleic (48.1–53.4%), linoleic (21.4–25.1%), and palmitic acids (13.0–15.8%). In these conditions, the yeast mainly synthesized torulene (43.5–47.7%) and β-carotene (34.7–38.6%), whereas the contribution of torularhodin was only 12.1–16.8%. Cultivation in medium with initial pH 3.0 resulted in a reduction in growth (13.0 gd.w./L) and total carotenoid (115.8 μg/gd.w.), linoleic acid (11.5%), and torularhodin (4.5%) biosynthesis. Conclusion: The different values of initial pH of the culture medium with glycerol and deproteinized potato wastewater had a significant effect on the growth and protein, lipid, and carotenoid biosynthesis by R. glutinis.
topic Carotenoids
Single cell oil
Single cell protein
Wastes
Medium pH
url http://www.sciencedirect.com/science/article/pii/S0717345817300076
work_keys_str_mv AT annamkot effectofinitialphofmediumwithpotatowastewaterandglycerolonproteinlipidandcarotenoidbiosynthesisbyrhodotorulaglutinis
AT stanisławbłazejak effectofinitialphofmediumwithpotatowastewaterandglycerolonproteinlipidandcarotenoidbiosynthesisbyrhodotorulaglutinis
AT agnieszkakurcz effectofinitialphofmediumwithpotatowastewaterandglycerolonproteinlipidandcarotenoidbiosynthesisbyrhodotorulaglutinis
AT joannabrys effectofinitialphofmediumwithpotatowastewaterandglycerolonproteinlipidandcarotenoidbiosynthesisbyrhodotorulaglutinis
AT iwonagientka effectofinitialphofmediumwithpotatowastewaterandglycerolonproteinlipidandcarotenoidbiosynthesisbyrhodotorulaglutinis
AT annabzduchawrobel effectofinitialphofmediumwithpotatowastewaterandglycerolonproteinlipidandcarotenoidbiosynthesisbyrhodotorulaglutinis
AT magdalenamaliszewska effectofinitialphofmediumwithpotatowastewaterandglycerolonproteinlipidandcarotenoidbiosynthesisbyrhodotorulaglutinis
AT lidiareczek effectofinitialphofmediumwithpotatowastewaterandglycerolonproteinlipidandcarotenoidbiosynthesisbyrhodotorulaglutinis
_version_ 1716810553776668672