Modification of a bi-functional diguanylate cyclase-phosphodiesterase to efficiently produce cyclic diguanylate monophosphate
Cyclic-diGMP is a bacterial messenger that regulates many physiological processes, including many attributed to pathogenicity. Bacteria synthesize cyclic-diGMP from GTP using diguanylate cyclases; its hydrolysis is catalyzed by phosphodiesterases. Here we report the over-expression and purification...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2015-09-01
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Series: | Biotechnology Reports |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X15000272 |