Modification of a bi-functional diguanylate cyclase-phosphodiesterase to efficiently produce cyclic diguanylate monophosphate

Cyclic-diGMP is a bacterial messenger that regulates many physiological processes, including many attributed to pathogenicity. Bacteria synthesize cyclic-diGMP from GTP using diguanylate cyclases; its hydrolysis is catalyzed by phosphodiesterases. Here we report the over-expression and purification...

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Bibliographic Details
Main Authors: Natasha M. Nesbitt, Dhruv P. Arora, Roger A. Johnson, Elizabeth M. Boon
Format: Article
Language:English
Published: Elsevier 2015-09-01
Series:Biotechnology Reports
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2215017X15000272