Sulforaphane inhibits self-renewal of lung cancer stem cells through the modulation of sonic Hedgehog signaling pathway and polyhomeotic homolog 3
Abstract Sulforaphane (SFN), an active compound in cruciferous vegetables, has been characterized by its antiproliferative capacity. We investigated the role and molecular mechanism through which SFN regulates proliferation and self-renewal of lung cancer stem cells. CD133+ cells were isolated with...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
SpringerOpen
2021-08-01
|
Series: | AMB Express |
Subjects: | |
Online Access: | https://doi.org/10.1186/s13568-021-01281-x |
id |
doaj-8be10a185f7648b4b13930d6fbbcac3e |
---|---|
record_format |
Article |
spelling |
doaj-8be10a185f7648b4b13930d6fbbcac3e2021-08-29T11:15:24ZengSpringerOpenAMB Express2191-08552021-08-0111111010.1186/s13568-021-01281-xSulforaphane inhibits self-renewal of lung cancer stem cells through the modulation of sonic Hedgehog signaling pathway and polyhomeotic homolog 3Fanping Wang0Yanwei Sun1Xiaoyu Huang2Caijuan Qiao3Wenrui Zhang4Peijun Liu5Mingyong Wang6Henan Key Laboratory of Immunology and Targeted Drug, School of Laboratory Medicine, Xinxiang Medical UniversityHenan Key Laboratory of Immunology and Targeted Drug, School of Laboratory Medicine, Xinxiang Medical UniversityHenan Key Laboratory of Immunology and Targeted Drug, School of Laboratory Medicine, Xinxiang Medical UniversityHenan Key Laboratory of Immunology and Targeted Drug, School of Laboratory Medicine, Xinxiang Medical UniversityHenan Key Laboratory of Immunology and Targeted Drug, School of Laboratory Medicine, Xinxiang Medical UniversityKey Laboratory for Tumor Precision Medicine of Shaanxi Province, First Affiliated Hospital of Xi’an Jiaotong UniversityHenan Key Laboratory of Immunology and Targeted Drug, School of Laboratory Medicine, Xinxiang Medical UniversityAbstract Sulforaphane (SFN), an active compound in cruciferous vegetables, has been characterized by its antiproliferative capacity. We investigated the role and molecular mechanism through which SFN regulates proliferation and self-renewal of lung cancer stem cells. CD133+ cells were isolated with MACs from lung cancer A549 and H460 cells. In this study, we found that SFN inhibited the proliferation of lung cancer cells and self-renewal of lung cancer stem cells simultaneously. Meanwhile, the mRNA and protein expressions of Shh, Smo, Gli1 and PHC3 were highly activated in CD133+ lung cancer cells. Compared with siRNA-control group, Knock-down of Shh inhibited proliferation of CD133+ lung cancer cells, and decreased the protein expression of PHC3 in CD133+ lung cancer cells. Knock-down of PHC3 also affected the proliferation and decreased the Shh expression level in CD133+ lung cancer cells. In addition, SFN inhibited the activities of Shh, Smo, Gli1 and PHC3 in CD133+ lung cancer cells. Furthermore, the inhibitory effect of SFN on the proliferation of siRNA-Shh and siRNA-PHC3 cells was weaker than that on the proliferation of siRNA-control cells. Sonic Hedgehog signaling pathway might undergo a cross-talk with PHC3 in self-renewal of lung cancer stem cells. SFN might be an effective new drug which could inhibit self-renewal of lung cancer stem cells through the modulation of Sonic Hedgehog signaling pathways and PHC3. This study could provide a novel way to improve therapeutic efficacy for lung cancer stem cells.https://doi.org/10.1186/s13568-021-01281-xSulforaphaneLung cancerCancer stem cellSonic Hedgehog Signaling PathwayHuman polyhomeotic homolog 3 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fanping Wang Yanwei Sun Xiaoyu Huang Caijuan Qiao Wenrui Zhang Peijun Liu Mingyong Wang |
spellingShingle |
Fanping Wang Yanwei Sun Xiaoyu Huang Caijuan Qiao Wenrui Zhang Peijun Liu Mingyong Wang Sulforaphane inhibits self-renewal of lung cancer stem cells through the modulation of sonic Hedgehog signaling pathway and polyhomeotic homolog 3 AMB Express Sulforaphane Lung cancer Cancer stem cell Sonic Hedgehog Signaling Pathway Human polyhomeotic homolog 3 |
author_facet |
Fanping Wang Yanwei Sun Xiaoyu Huang Caijuan Qiao Wenrui Zhang Peijun Liu Mingyong Wang |
author_sort |
Fanping Wang |
title |
Sulforaphane inhibits self-renewal of lung cancer stem cells through the modulation of sonic Hedgehog signaling pathway and polyhomeotic homolog 3 |
title_short |
Sulforaphane inhibits self-renewal of lung cancer stem cells through the modulation of sonic Hedgehog signaling pathway and polyhomeotic homolog 3 |
title_full |
Sulforaphane inhibits self-renewal of lung cancer stem cells through the modulation of sonic Hedgehog signaling pathway and polyhomeotic homolog 3 |
title_fullStr |
Sulforaphane inhibits self-renewal of lung cancer stem cells through the modulation of sonic Hedgehog signaling pathway and polyhomeotic homolog 3 |
title_full_unstemmed |
Sulforaphane inhibits self-renewal of lung cancer stem cells through the modulation of sonic Hedgehog signaling pathway and polyhomeotic homolog 3 |
title_sort |
sulforaphane inhibits self-renewal of lung cancer stem cells through the modulation of sonic hedgehog signaling pathway and polyhomeotic homolog 3 |
publisher |
SpringerOpen |
series |
AMB Express |
issn |
2191-0855 |
publishDate |
2021-08-01 |
description |
Abstract Sulforaphane (SFN), an active compound in cruciferous vegetables, has been characterized by its antiproliferative capacity. We investigated the role and molecular mechanism through which SFN regulates proliferation and self-renewal of lung cancer stem cells. CD133+ cells were isolated with MACs from lung cancer A549 and H460 cells. In this study, we found that SFN inhibited the proliferation of lung cancer cells and self-renewal of lung cancer stem cells simultaneously. Meanwhile, the mRNA and protein expressions of Shh, Smo, Gli1 and PHC3 were highly activated in CD133+ lung cancer cells. Compared with siRNA-control group, Knock-down of Shh inhibited proliferation of CD133+ lung cancer cells, and decreased the protein expression of PHC3 in CD133+ lung cancer cells. Knock-down of PHC3 also affected the proliferation and decreased the Shh expression level in CD133+ lung cancer cells. In addition, SFN inhibited the activities of Shh, Smo, Gli1 and PHC3 in CD133+ lung cancer cells. Furthermore, the inhibitory effect of SFN on the proliferation of siRNA-Shh and siRNA-PHC3 cells was weaker than that on the proliferation of siRNA-control cells. Sonic Hedgehog signaling pathway might undergo a cross-talk with PHC3 in self-renewal of lung cancer stem cells. SFN might be an effective new drug which could inhibit self-renewal of lung cancer stem cells through the modulation of Sonic Hedgehog signaling pathways and PHC3. This study could provide a novel way to improve therapeutic efficacy for lung cancer stem cells. |
topic |
Sulforaphane Lung cancer Cancer stem cell Sonic Hedgehog Signaling Pathway Human polyhomeotic homolog 3 |
url |
https://doi.org/10.1186/s13568-021-01281-x |
work_keys_str_mv |
AT fanpingwang sulforaphaneinhibitsselfrenewaloflungcancerstemcellsthroughthemodulationofsonichedgehogsignalingpathwayandpolyhomeotichomolog3 AT yanweisun sulforaphaneinhibitsselfrenewaloflungcancerstemcellsthroughthemodulationofsonichedgehogsignalingpathwayandpolyhomeotichomolog3 AT xiaoyuhuang sulforaphaneinhibitsselfrenewaloflungcancerstemcellsthroughthemodulationofsonichedgehogsignalingpathwayandpolyhomeotichomolog3 AT caijuanqiao sulforaphaneinhibitsselfrenewaloflungcancerstemcellsthroughthemodulationofsonichedgehogsignalingpathwayandpolyhomeotichomolog3 AT wenruizhang sulforaphaneinhibitsselfrenewaloflungcancerstemcellsthroughthemodulationofsonichedgehogsignalingpathwayandpolyhomeotichomolog3 AT peijunliu sulforaphaneinhibitsselfrenewaloflungcancerstemcellsthroughthemodulationofsonichedgehogsignalingpathwayandpolyhomeotichomolog3 AT mingyongwang sulforaphaneinhibitsselfrenewaloflungcancerstemcellsthroughthemodulationofsonichedgehogsignalingpathwayandpolyhomeotichomolog3 |
_version_ |
1714332115801210880 |