Effects of Hair Follicle Stem Cells on Partial-Thickness Burn Wound Healing and Tensile Strength
Background: The recent improvements in wound healing have led to new strategies in regenerative medicine. Burn wound healing is an important issue in skin regeneration and has multiple indications for stem cell therapy. HFSCs are a highly promising source of stem cells for healing use, as these cell...
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doaj-8cbc8e067c964697b733d9b79c3e6d532020-11-25T02:00:06ZengPasteur Institute of IranIranian Biomedical Journal1028-852X2008-823X2020-03-012429910910.29252/ibj.24.2.99Effects of Hair Follicle Stem Cells on Partial-Thickness Burn Wound Healing and Tensile StrengthAzar Babakhani0Malihe Nobakht1Hamidreza Pazoki Torodi2Mostafa Dahmardehei3Paria Hashemi4Javad Mohajer Ansari5Parisa Ramhormozi6Abazar Yari7Fatemeh Heidari8Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IranDepartment of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IranPhysiology Research Center, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IranZahedan Medical University, Zahedan, Iran; 4Department of Anatomy, Faculty of Medicine, Alborz University of Medical Sciences, Karaj, IranPhysiology Research Center, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IranDepartment of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IranDepartment of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IranDepartment of Anatomy, Faculty of Medicine, Alborz University of Medical Sciences, Karaj, IranDepartment of Anatomy, Faculty of Medicine, Qum University of Medical Sciences, Qum, IranBackground: The recent improvements in wound healing have led to new strategies in regenerative medicine. Burn wound healing is an important issue in skin regeneration and has multiple indications for stem cell therapy. HFSCs are a highly promising source of stem cells for healing use, as these cells are accessible, active and pluripotent adult stem cells. Methods: HFSCs of the rat whisker were isolated, cultured, and labeled with DiI. Flow cytometry method was used to detect special markers of HFSCs. Deep partial-thickness burn wound was created, and labeled HFSCs were injected around the wound bed. Wound closure was recorded via digital photographs. The inflicted rats were sacrificed at 3, 7, or 14 days post burn and used for subsequent histological and tensiometry analysis. Results: Our results indicated that HFSCs were positive for Nestin and CD34 markers, but negative for Kr15. Morphological and histological photographs revealed that wound closure rate was accelerated in stem cell-treated group compared with other groups. In addition, faster re-epithelialization and collagen deposition were observed. The immunohistochemical analysis suggested that CD31 expression and vascular density enhanced in the stem cell-treated group. Further, tissue tensile strength increased in HFSCs-treated rats in comparison to the control group. Conclusion: The present study demonstrates that HFSCs could accelerate burn wound healing as well as tensile strength in rats.http://ibj.pasteur.ac.ir/article-1-2816-en.pdfburn wound healingepithelizationtensile strength |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Azar Babakhani Malihe Nobakht Hamidreza Pazoki Torodi Mostafa Dahmardehei Paria Hashemi Javad Mohajer Ansari Parisa Ramhormozi Abazar Yari Fatemeh Heidari |
spellingShingle |
Azar Babakhani Malihe Nobakht Hamidreza Pazoki Torodi Mostafa Dahmardehei Paria Hashemi Javad Mohajer Ansari Parisa Ramhormozi Abazar Yari Fatemeh Heidari Effects of Hair Follicle Stem Cells on Partial-Thickness Burn Wound Healing and Tensile Strength Iranian Biomedical Journal burn wound healing epithelization tensile strength |
author_facet |
Azar Babakhani Malihe Nobakht Hamidreza Pazoki Torodi Mostafa Dahmardehei Paria Hashemi Javad Mohajer Ansari Parisa Ramhormozi Abazar Yari Fatemeh Heidari |
author_sort |
Azar Babakhani |
title |
Effects of Hair Follicle Stem Cells on Partial-Thickness Burn Wound Healing and Tensile Strength |
title_short |
Effects of Hair Follicle Stem Cells on Partial-Thickness Burn Wound Healing and Tensile Strength |
title_full |
Effects of Hair Follicle Stem Cells on Partial-Thickness Burn Wound Healing and Tensile Strength |
title_fullStr |
Effects of Hair Follicle Stem Cells on Partial-Thickness Burn Wound Healing and Tensile Strength |
title_full_unstemmed |
Effects of Hair Follicle Stem Cells on Partial-Thickness Burn Wound Healing and Tensile Strength |
title_sort |
effects of hair follicle stem cells on partial-thickness burn wound healing and tensile strength |
publisher |
Pasteur Institute of Iran |
series |
Iranian Biomedical Journal |
issn |
1028-852X 2008-823X |
publishDate |
2020-03-01 |
description |
Background: The recent improvements in wound healing have led to new strategies in regenerative medicine. Burn wound healing is an important issue in skin regeneration and has multiple indications for stem cell therapy. HFSCs are a highly promising source of stem cells for healing use, as these cells are accessible, active and pluripotent adult stem cells. Methods: HFSCs of the rat whisker were isolated, cultured, and labeled with DiI. Flow cytometry method was used to detect special markers of HFSCs. Deep partial-thickness burn wound was created, and labeled HFSCs were injected around the wound bed. Wound closure was recorded via digital photographs. The inflicted rats were sacrificed at 3, 7, or 14 days post burn and used for subsequent histological and tensiometry analysis. Results: Our results indicated that HFSCs were positive for Nestin and CD34 markers, but negative for Kr15. Morphological and histological photographs revealed that wound closure rate was accelerated in stem cell-treated group compared with other groups. In addition, faster re-epithelialization and collagen deposition were observed. The immunohistochemical analysis suggested that CD31 expression and vascular density enhanced in the stem cell-treated group. Further, tissue tensile strength increased in HFSCs-treated rats in comparison to the control group. Conclusion: The present study demonstrates that HFSCs could accelerate burn wound healing as well as tensile strength in rats. |
topic |
burn wound healing epithelization tensile strength |
url |
http://ibj.pasteur.ac.ir/article-1-2816-en.pdf |
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