RH phenotype, ABO and kell antigens, alleles and haplotypes frequencies in North Indian blood donor population

Background and Objectives: ABO, RH and Kell blood group systems are clinically significant blood group systems among 36 blood group systems having total of 360 blood group antigens which cause most cases of alloimmunization following blood transfusions or pregnancy. India has regionally and ethnical...

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Main Authors: Sadhana Mangwana, Nikhil Simon, Lalitesh Sangwan
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2021-01-01
Series:Global Journal of Transfusion Medicine
Subjects:
abo
Online Access:http://www.gjtmonline.com/article.asp?issn=2468-8398;year=2021;volume=6;issue=1;spage=81;epage=85;aulast=Mangwana
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spelling doaj-8ccaa2866b1d4b7280f21ab77c8c8c482021-06-15T04:29:41ZengWolters Kluwer Medknow PublicationsGlobal Journal of Transfusion Medicine2468-83982455-88932021-01-0161818510.4103/gjtm.gjtm_23_21RH phenotype, ABO and kell antigens, alleles and haplotypes frequencies in North Indian blood donor populationSadhana MangwanaNikhil SimonLalitesh SangwanBackground and Objectives: ABO, RH and Kell blood group systems are clinically significant blood group systems among 36 blood group systems having total of 360 blood group antigens which cause most cases of alloimmunization following blood transfusions or pregnancy. India has regionally and ethnically very diverse population in different demographic areas. Due to different antigenic expression in different population, knowledge of antigen frequencies among their local and regional donor population is important to assess the risk of antibody formation, probability of antigen negative blood for patients having alloantibodies, inventory management and database for rare antigens thus enhancing patient safety. Aim: To know and compare phenotype prevalence, frequency distribution of ABO, RH and Kell antigens and Alleles in blood donors. Methods: A prospective study was conducted in a tertiary care hospital in North India over three years period. ABO, RH D grouping, RH and Kell phenotypes were performed on blood donors. Hardy Weinberg's equations were used to calculate ABO, RH and Kell allele frequencies and were analyzed. Results: During the study period, 24745 healthy individuals donated blood at Tertiary Care hospital; with 97.26% male and 2.74% female population. Prevalence of ABO antigens were B =37.74%, O = 31.38%, A = 21.77% and AB = 9.09% with allele frequency for O = 0.560, B = 0.266, A = 0.169 and AB = 0.091. The commonest RH phenotype observed was DCCee followed by DCcee, DCcEe, DccEe while commonest RH negative phenotype were ccee and Ccee. Homozygous phenotypes were absent in our population. Amongst five RH antigens phenotyped serologically, highest prevalence was of e antigen followed by D, C, c and E being the lowest. Prevalence of Kell phenotype was 2.57% (K) while that of k as 97.43% with allele frequencies of K and k as 0.0252 and 0.986 respectively. Discussion: Genetic variability in different population result in varied expression of red cell antigens in different races. Knowledge of varied frequency and phenotypic expression of major clinically relevant antigens and RH haplotypes in these blood groups systems would help in more rational approach for blood transfusion, decreasing alloimmunization thus enhancing blood safety.http://www.gjtmonline.com/article.asp?issn=2468-8398;year=2021;volume=6;issue=1;spage=81;epage=85;aulast=Mangwanaaboalleles and haplotypeskell antigensrh phenotype
collection DOAJ
language English
format Article
sources DOAJ
author Sadhana Mangwana
Nikhil Simon
Lalitesh Sangwan
spellingShingle Sadhana Mangwana
Nikhil Simon
Lalitesh Sangwan
RH phenotype, ABO and kell antigens, alleles and haplotypes frequencies in North Indian blood donor population
Global Journal of Transfusion Medicine
abo
alleles and haplotypes
kell antigens
rh phenotype
author_facet Sadhana Mangwana
Nikhil Simon
Lalitesh Sangwan
author_sort Sadhana Mangwana
title RH phenotype, ABO and kell antigens, alleles and haplotypes frequencies in North Indian blood donor population
title_short RH phenotype, ABO and kell antigens, alleles and haplotypes frequencies in North Indian blood donor population
title_full RH phenotype, ABO and kell antigens, alleles and haplotypes frequencies in North Indian blood donor population
title_fullStr RH phenotype, ABO and kell antigens, alleles and haplotypes frequencies in North Indian blood donor population
title_full_unstemmed RH phenotype, ABO and kell antigens, alleles and haplotypes frequencies in North Indian blood donor population
title_sort rh phenotype, abo and kell antigens, alleles and haplotypes frequencies in north indian blood donor population
publisher Wolters Kluwer Medknow Publications
series Global Journal of Transfusion Medicine
issn 2468-8398
2455-8893
publishDate 2021-01-01
description Background and Objectives: ABO, RH and Kell blood group systems are clinically significant blood group systems among 36 blood group systems having total of 360 blood group antigens which cause most cases of alloimmunization following blood transfusions or pregnancy. India has regionally and ethnically very diverse population in different demographic areas. Due to different antigenic expression in different population, knowledge of antigen frequencies among their local and regional donor population is important to assess the risk of antibody formation, probability of antigen negative blood for patients having alloantibodies, inventory management and database for rare antigens thus enhancing patient safety. Aim: To know and compare phenotype prevalence, frequency distribution of ABO, RH and Kell antigens and Alleles in blood donors. Methods: A prospective study was conducted in a tertiary care hospital in North India over three years period. ABO, RH D grouping, RH and Kell phenotypes were performed on blood donors. Hardy Weinberg's equations were used to calculate ABO, RH and Kell allele frequencies and were analyzed. Results: During the study period, 24745 healthy individuals donated blood at Tertiary Care hospital; with 97.26% male and 2.74% female population. Prevalence of ABO antigens were B =37.74%, O = 31.38%, A = 21.77% and AB = 9.09% with allele frequency for O = 0.560, B = 0.266, A = 0.169 and AB = 0.091. The commonest RH phenotype observed was DCCee followed by DCcee, DCcEe, DccEe while commonest RH negative phenotype were ccee and Ccee. Homozygous phenotypes were absent in our population. Amongst five RH antigens phenotyped serologically, highest prevalence was of e antigen followed by D, C, c and E being the lowest. Prevalence of Kell phenotype was 2.57% (K) while that of k as 97.43% with allele frequencies of K and k as 0.0252 and 0.986 respectively. Discussion: Genetic variability in different population result in varied expression of red cell antigens in different races. Knowledge of varied frequency and phenotypic expression of major clinically relevant antigens and RH haplotypes in these blood groups systems would help in more rational approach for blood transfusion, decreasing alloimmunization thus enhancing blood safety.
topic abo
alleles and haplotypes
kell antigens
rh phenotype
url http://www.gjtmonline.com/article.asp?issn=2468-8398;year=2021;volume=6;issue=1;spage=81;epage=85;aulast=Mangwana
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AT laliteshsangwan rhphenotypeaboandkellantigensallelesandhaplotypesfrequenciesinnorthindianblooddonorpopulation
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