Analysis of excreta bacterial community after forced molting in aged laying hens
Objective As laying hens become aged, laying performance and egg quality are generally impaired. One of the practical methods to rejuvenate production and egg quality of aged laying hens with decreasing productivity is a forced molting. However, the changes in intestinal microbiota after forced molt...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Asian-Australasian Association of Animal Production Societies
2019-11-01
|
Series: | Asian-Australasian Journal of Animal Sciences |
Subjects: | |
Online Access: | http://www.ajas.info/upload/pdf/ajas-19-0180.pdf |
id |
doaj-8dd9bcc6bf1e4a97a425fff15062e1e6 |
---|---|
record_format |
Article |
spelling |
doaj-8dd9bcc6bf1e4a97a425fff15062e1e62020-11-25T02:38:51ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172019-11-0132111715172410.5713/ajas.19.018024290Analysis of excreta bacterial community after forced molting in aged laying hensGi Ppeum Han0Kyu-Chan Lee1Hwan Ku Kang2Han Na Oh3Woo Jun Sul4Dong Yong Kil5 Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea Department of Systems Biotechnology, Chung-Ang University, Anseong 17546, Korea Poultry Research Institute, National Institute of Animal Science, Rural Development Administration, Pyeongchang 25342, Korea Department of Systems Biotechnology, Chung-Ang University, Anseong 17546, Korea Department of Systems Biotechnology, Chung-Ang University, Anseong 17546, Korea Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, KoreaObjective As laying hens become aged, laying performance and egg quality are generally impaired. One of the practical methods to rejuvenate production and egg quality of aged laying hens with decreasing productivity is a forced molting. However, the changes in intestinal microbiota after forced molting of aged hens are not clearly known. The aim of the present study was to analyze the changes in excreta bacterial communities after forced molting of aged laying hens. Methods A total of one hundred 66-wk-old Hy-Line Brown laying hens were induced to molt by a 2-d water removal and an 11-d fasting until egg production completely ceased. The excreta samples of 16 hens with similar body weight were collected before and immediately after molting. Excreta bacterial communities were analyzed by high-throughput sequencing of bacterial 16S rRNA genes. Results Bacteroidetes, Firmicutes, and Proteobacteria were the three major bacterial phyla in pre-molting and immediate post-molting hens, accounting for more than 98.0%. Lactobacillus genus had relatively high abundance in both group, but decreased by molting (62.3% in pre-molting and 24.9% in post-molting hens). Moreover, pathogenic bacteria such as Enterococcus cecorum and Escherichia coli were more abundant in immediate post-molting hens than in pre-molting hens. Forced molting influenced the alpha diversity, with higher Chao1 (p = 0.012), phylogenetic diversity whole tree (p = 0.014), observed operational taxonomic unit indices (p = 0.006), and Simpson indices (p<0.001), which indicated that forced molting increased excreta bacterial richness of aged laying hens. Conclusion This study improves the current knowledge of bacterial community alterations in the excreta by forced molting in aged laying hens, which can provide increasing opportunity to develop novel dietary and management skills for improving the gastrointestinal health of aged laying hens after molting.http://www.ajas.info/upload/pdf/ajas-19-0180.pdfaged laying henexcreta bacterial communityforced moltinghigh-throughput 16s rrna sequencing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gi Ppeum Han Kyu-Chan Lee Hwan Ku Kang Han Na Oh Woo Jun Sul Dong Yong Kil |
spellingShingle |
Gi Ppeum Han Kyu-Chan Lee Hwan Ku Kang Han Na Oh Woo Jun Sul Dong Yong Kil Analysis of excreta bacterial community after forced molting in aged laying hens Asian-Australasian Journal of Animal Sciences aged laying hen excreta bacterial community forced molting high-throughput 16s rrna sequencing |
author_facet |
Gi Ppeum Han Kyu-Chan Lee Hwan Ku Kang Han Na Oh Woo Jun Sul Dong Yong Kil |
author_sort |
Gi Ppeum Han |
title |
Analysis of excreta bacterial community after forced molting in aged laying hens |
title_short |
Analysis of excreta bacterial community after forced molting in aged laying hens |
title_full |
Analysis of excreta bacterial community after forced molting in aged laying hens |
title_fullStr |
Analysis of excreta bacterial community after forced molting in aged laying hens |
title_full_unstemmed |
Analysis of excreta bacterial community after forced molting in aged laying hens |
title_sort |
analysis of excreta bacterial community after forced molting in aged laying hens |
publisher |
Asian-Australasian Association of Animal Production Societies |
series |
Asian-Australasian Journal of Animal Sciences |
issn |
1011-2367 1976-5517 |
publishDate |
2019-11-01 |
description |
Objective As laying hens become aged, laying performance and egg quality are generally impaired. One of the practical methods to rejuvenate production and egg quality of aged laying hens with decreasing productivity is a forced molting. However, the changes in intestinal microbiota after forced molting of aged hens are not clearly known. The aim of the present study was to analyze the changes in excreta bacterial communities after forced molting of aged laying hens. Methods A total of one hundred 66-wk-old Hy-Line Brown laying hens were induced to molt by a 2-d water removal and an 11-d fasting until egg production completely ceased. The excreta samples of 16 hens with similar body weight were collected before and immediately after molting. Excreta bacterial communities were analyzed by high-throughput sequencing of bacterial 16S rRNA genes. Results Bacteroidetes, Firmicutes, and Proteobacteria were the three major bacterial phyla in pre-molting and immediate post-molting hens, accounting for more than 98.0%. Lactobacillus genus had relatively high abundance in both group, but decreased by molting (62.3% in pre-molting and 24.9% in post-molting hens). Moreover, pathogenic bacteria such as Enterococcus cecorum and Escherichia coli were more abundant in immediate post-molting hens than in pre-molting hens. Forced molting influenced the alpha diversity, with higher Chao1 (p = 0.012), phylogenetic diversity whole tree (p = 0.014), observed operational taxonomic unit indices (p = 0.006), and Simpson indices (p<0.001), which indicated that forced molting increased excreta bacterial richness of aged laying hens. Conclusion This study improves the current knowledge of bacterial community alterations in the excreta by forced molting in aged laying hens, which can provide increasing opportunity to develop novel dietary and management skills for improving the gastrointestinal health of aged laying hens after molting. |
topic |
aged laying hen excreta bacterial community forced molting high-throughput 16s rrna sequencing |
url |
http://www.ajas.info/upload/pdf/ajas-19-0180.pdf |
work_keys_str_mv |
AT gippeumhan analysisofexcretabacterialcommunityafterforcedmoltinginagedlayinghens AT kyuchanlee analysisofexcretabacterialcommunityafterforcedmoltinginagedlayinghens AT hwankukang analysisofexcretabacterialcommunityafterforcedmoltinginagedlayinghens AT hannaoh analysisofexcretabacterialcommunityafterforcedmoltinginagedlayinghens AT woojunsul analysisofexcretabacterialcommunityafterforcedmoltinginagedlayinghens AT dongyongkil analysisofexcretabacterialcommunityafterforcedmoltinginagedlayinghens |
_version_ |
1724789390916452352 |