Decontamination of digital image sensors and assessment of electron microscope performance in a BSL-3 containment
A unique biological safety level (BSL)-3 cryo-electron microscopy facility with a 200 keV high-end cryo-electron microscope has been commissioned at the University of Texas Medical Branch (UTMB) to study the structure of viruses and bacteria classified as select agents. We developed a microscope dec...
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AIMS Press
2015-05-01
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| Series: | AIMS Biophysics |
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| Online Access: | http://www.aimspress.com/biophysics/article/285/fulltext.html |
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doaj-8f5e0bf2888e403caad528ccee656a172020-11-25T00:19:04ZengAIMS PressAIMS Biophysics2377-90982015-05-012215316210.3934/biophy.2015.2.153201502153Decontamination of digital image sensors and assessment of electron microscope performance in a BSL-3 containmentMichael B. ShermanJuan Trujillo0Benjamin E. Bammes1Liang Jin2Matthias W. Stumpf3Scott C. WeaverJEOL USA, Inc., Peabody, Massachusetts, USADirect Electron, LP, San Diego, California, USADirect Electron, LP, San Diego, California, USAE. A. Fischione Instruments, Inc., Export, Pennsylvania, USAA unique biological safety level (BSL)-3 cryo-electron microscopy facility with a 200 keV high-end cryo-electron microscope has been commissioned at the University of Texas Medical Branch (UTMB) to study the structure of viruses and bacteria classified as select agents. We developed a microscope decontamination protocol based on chlorine dioxide gas with a continuous flow system. In this paper we report on testing digital camera sensors (both CCD and CMOS direct detector) in a BSL-3 environment, and microscope performance after chlorine dioxide (ClO<sub>2</sub>) decontamination cycles.http://www.aimspress.com/biophysics/article/285/fulltext.htmlcryo-electron microscopysingle particle imagingbiological safety containment |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Michael B. Sherman Juan Trujillo Benjamin E. Bammes Liang Jin Matthias W. Stumpf Scott C. Weaver |
| spellingShingle |
Michael B. Sherman Juan Trujillo Benjamin E. Bammes Liang Jin Matthias W. Stumpf Scott C. Weaver Decontamination of digital image sensors and assessment of electron microscope performance in a BSL-3 containment AIMS Biophysics cryo-electron microscopy single particle imaging biological safety containment |
| author_facet |
Michael B. Sherman Juan Trujillo Benjamin E. Bammes Liang Jin Matthias W. Stumpf Scott C. Weaver |
| author_sort |
Michael B. Sherman |
| title |
Decontamination of digital image sensors and assessment of electron microscope performance in a BSL-3 containment |
| title_short |
Decontamination of digital image sensors and assessment of electron microscope performance in a BSL-3 containment |
| title_full |
Decontamination of digital image sensors and assessment of electron microscope performance in a BSL-3 containment |
| title_fullStr |
Decontamination of digital image sensors and assessment of electron microscope performance in a BSL-3 containment |
| title_full_unstemmed |
Decontamination of digital image sensors and assessment of electron microscope performance in a BSL-3 containment |
| title_sort |
decontamination of digital image sensors and assessment of electron microscope performance in a bsl-3 containment |
| publisher |
AIMS Press |
| series |
AIMS Biophysics |
| issn |
2377-9098 |
| publishDate |
2015-05-01 |
| description |
A unique biological safety level (BSL)-3 cryo-electron microscopy facility with a 200 keV high-end cryo-electron microscope has been commissioned at the University of Texas Medical Branch (UTMB) to study the structure of viruses and bacteria classified as select agents. We developed a microscope decontamination protocol based on chlorine dioxide gas with a continuous flow system. In this paper we report on testing digital camera sensors (both CCD and CMOS direct detector) in a BSL-3 environment, and microscope performance after chlorine dioxide (ClO<sub>2</sub>) decontamination cycles. |
| topic |
cryo-electron microscopy single particle imaging biological safety containment |
| url |
http://www.aimspress.com/biophysics/article/285/fulltext.html |
| work_keys_str_mv |
AT michaelbsherman decontaminationofdigitalimagesensorsandassessmentofelectronmicroscopeperformanceinabsl3containment AT juantrujillo decontaminationofdigitalimagesensorsandassessmentofelectronmicroscopeperformanceinabsl3containment AT benjaminebammes decontaminationofdigitalimagesensorsandassessmentofelectronmicroscopeperformanceinabsl3containment AT liangjin decontaminationofdigitalimagesensorsandassessmentofelectronmicroscopeperformanceinabsl3containment AT matthiaswstumpf decontaminationofdigitalimagesensorsandassessmentofelectronmicroscopeperformanceinabsl3containment AT scottcweaver decontaminationofdigitalimagesensorsandassessmentofelectronmicroscopeperformanceinabsl3containment |
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