Development of droplet digital PCR for the detection of Tilletia laevis, which causes common bunt of wheat, based on the SCAR marker derived from ISSR and real-time PCR

Abstract Common bunt of wheat caused by Tilletia laevis and/or T. caries (syn. T. tritici), is a major disease in wheat-growing regions worldwide that could lead to 80% or even total loss of production. Even though T. laevis can be distinguished from T. caries on the bases of morphology of teliospor...

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Bibliographic Details
Main Authors: Tongshuo Xu, Zhaoqun Yao, Jianjian Liu, Han Zhang, Ghulam Muhae Ud Din, Sifeng Zhao, Wanquan Chen, Taiguo Liu, Li Gao
Format: Article
Language:English
Published: Nature Publishing Group 2020-09-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-020-72976-7