Heater Integrated Lab-on-a-Chip Device for Rapid HLA Alleles Amplification towards Prevention of Drug Hypersensitivity

HLA-B*15:02 screening before administering carbamazepine is recommended to prevent life-threatening hypersensitivity. However, the unavailability of a point-of-care device impedes this screening process. Our research group previously developed a two-step HLA-B*15:02 detection technique utilizing loo...

Full description

Bibliographic Details
Main Authors: Shah Mukim Uddin, Abkar Sayad, Jianxiong Chan, Duc Hau Huynh, Efstratios Skafidas, Patrick Kwan
Format: Article
Language:English
Published: MDPI AG 2021-05-01
Series:Sensors
Subjects:
Online Access:https://www.mdpi.com/1424-8220/21/10/3413
id doaj-90a78996e4944ec2a3bcd45f5209948d
record_format Article
spelling doaj-90a78996e4944ec2a3bcd45f5209948d2021-05-31T23:59:11ZengMDPI AGSensors1424-82202021-05-01213413341310.3390/s21103413Heater Integrated Lab-on-a-Chip Device for Rapid HLA Alleles Amplification towards Prevention of Drug HypersensitivityShah Mukim Uddin0Abkar Sayad1Jianxiong Chan2Duc Hau Huynh3Efstratios Skafidas4Patrick Kwan5Department of Medicine, The University of Melbourne, Royal Melbourne Hospital, Melbourne, VIC 3050, AustraliaDepartment of Neuroscience, Central Clinical School, Monash University, Melbourne, VIC 3004, AustraliaDepartment of Medicine, The University of Melbourne, Royal Melbourne Hospital, Melbourne, VIC 3050, AustraliaDepartment of Medicine, The University of Melbourne, Royal Melbourne Hospital, Melbourne, VIC 3050, AustraliaDepartment of Medicine, The University of Melbourne, Royal Melbourne Hospital, Melbourne, VIC 3050, AustraliaDepartment of Medicine, The University of Melbourne, Royal Melbourne Hospital, Melbourne, VIC 3050, AustraliaHLA-B*15:02 screening before administering carbamazepine is recommended to prevent life-threatening hypersensitivity. However, the unavailability of a point-of-care device impedes this screening process. Our research group previously developed a two-step HLA-B*15:02 detection technique utilizing loop-mediated isothermal amplification (LAMP) on the tube, which requires two-stage device development to translate into a portable platform. Here, we report a heater-integrated lab-on-a-chip device for the LAMP amplification, which can rapidly detect HLA-B alleles colorimetrically. A gold-patterned micro-sized heater was integrated into a 3D-printed chip, allowing microfluidic pumping, valving, and incubation. The performance of the chip was tested with color dye. Then LAMP assay was conducted with human genomic DNA samples of known HLA-B genotypes in the LAMP-chip parallel with the tube assay. The LAMP-on-chip results showed a complete match with the LAMP-on-tube assay, demonstrating the detection system’s concurrence.https://www.mdpi.com/1424-8220/21/10/3413loop-mediated isothermal amplificationmicrofluidicslab-on-a-chippoint-of-care diagnosticspharmaco-genetics
collection DOAJ
language English
format Article
sources DOAJ
author Shah Mukim Uddin
Abkar Sayad
Jianxiong Chan
Duc Hau Huynh
Efstratios Skafidas
Patrick Kwan
spellingShingle Shah Mukim Uddin
Abkar Sayad
Jianxiong Chan
Duc Hau Huynh
Efstratios Skafidas
Patrick Kwan
Heater Integrated Lab-on-a-Chip Device for Rapid HLA Alleles Amplification towards Prevention of Drug Hypersensitivity
Sensors
loop-mediated isothermal amplification
microfluidics
lab-on-a-chip
point-of-care diagnostics
pharmaco-genetics
author_facet Shah Mukim Uddin
Abkar Sayad
Jianxiong Chan
Duc Hau Huynh
Efstratios Skafidas
Patrick Kwan
author_sort Shah Mukim Uddin
title Heater Integrated Lab-on-a-Chip Device for Rapid HLA Alleles Amplification towards Prevention of Drug Hypersensitivity
title_short Heater Integrated Lab-on-a-Chip Device for Rapid HLA Alleles Amplification towards Prevention of Drug Hypersensitivity
title_full Heater Integrated Lab-on-a-Chip Device for Rapid HLA Alleles Amplification towards Prevention of Drug Hypersensitivity
title_fullStr Heater Integrated Lab-on-a-Chip Device for Rapid HLA Alleles Amplification towards Prevention of Drug Hypersensitivity
title_full_unstemmed Heater Integrated Lab-on-a-Chip Device for Rapid HLA Alleles Amplification towards Prevention of Drug Hypersensitivity
title_sort heater integrated lab-on-a-chip device for rapid hla alleles amplification towards prevention of drug hypersensitivity
publisher MDPI AG
series Sensors
issn 1424-8220
publishDate 2021-05-01
description HLA-B*15:02 screening before administering carbamazepine is recommended to prevent life-threatening hypersensitivity. However, the unavailability of a point-of-care device impedes this screening process. Our research group previously developed a two-step HLA-B*15:02 detection technique utilizing loop-mediated isothermal amplification (LAMP) on the tube, which requires two-stage device development to translate into a portable platform. Here, we report a heater-integrated lab-on-a-chip device for the LAMP amplification, which can rapidly detect HLA-B alleles colorimetrically. A gold-patterned micro-sized heater was integrated into a 3D-printed chip, allowing microfluidic pumping, valving, and incubation. The performance of the chip was tested with color dye. Then LAMP assay was conducted with human genomic DNA samples of known HLA-B genotypes in the LAMP-chip parallel with the tube assay. The LAMP-on-chip results showed a complete match with the LAMP-on-tube assay, demonstrating the detection system’s concurrence.
topic loop-mediated isothermal amplification
microfluidics
lab-on-a-chip
point-of-care diagnostics
pharmaco-genetics
url https://www.mdpi.com/1424-8220/21/10/3413
work_keys_str_mv AT shahmukimuddin heaterintegratedlabonachipdeviceforrapidhlaallelesamplificationtowardspreventionofdrughypersensitivity
AT abkarsayad heaterintegratedlabonachipdeviceforrapidhlaallelesamplificationtowardspreventionofdrughypersensitivity
AT jianxiongchan heaterintegratedlabonachipdeviceforrapidhlaallelesamplificationtowardspreventionofdrughypersensitivity
AT duchauhuynh heaterintegratedlabonachipdeviceforrapidhlaallelesamplificationtowardspreventionofdrughypersensitivity
AT efstratiosskafidas heaterintegratedlabonachipdeviceforrapidhlaallelesamplificationtowardspreventionofdrughypersensitivity
AT patrickkwan heaterintegratedlabonachipdeviceforrapidhlaallelesamplificationtowardspreventionofdrughypersensitivity
_version_ 1721416017887887360