The early detection of immunoglobulins via optical-based lateral flow immunoassay platform in COVID-19 pandemic.
The coronavirus disease (COVID-19) is the global public health challenge currently persisting at a grand scale. A method that meets the rapid quantitative detection of antibodies to assess the body's immune response from natural COVID-19 illness or vaccines' effects is urgently needed. In...
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doaj-952c6422139341d98b09061fc43ef4802021-08-05T04:30:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01167e025448610.1371/journal.pone.0254486The early detection of immunoglobulins via optical-based lateral flow immunoassay platform in COVID-19 pandemic.Pang-Yen ChenCheng-Hao KoC Jason WangChien-Wei ChenWei-Huai ChiuChitsung HongHao-Min ChengI-Jen WangThe coronavirus disease (COVID-19) is the global public health challenge currently persisting at a grand scale. A method that meets the rapid quantitative detection of antibodies to assess the body's immune response from natural COVID-19 illness or vaccines' effects is urgently needed. In the present study, an attempt was made to integrate a newly designed spectrometer to the COVID-19 test strip procedure; this augmentation provides the quantitative capacity to a lateral flow immunoassay (LFIA). Optical interpretation of results by quantitative α index, rather than visual qualification, can be done quickly, in 5-10 minutes. The developed product was compared with several other serological IgM/IgG antibody reagents on the market by recruiting 111 participants suspected of having COVID-19 infection from March to May 2020 in a hospital. Taking RT-PCR as the diagnostic gold standard, the quantitative spectral LIFA platform could correctly detect all 12 COVID-19 patients. Concerning RT-PCR negative patients, all three antibody testing methods found positive cases. The optical-based platform exhibited the ability of early detection of immunoglobulins of RT-PCR negative patients. There was an apparent trend that elevation of IgM levels in the acute phase of infection; then IgG levels rose later. It exhibited the risk of a false-negative diagnosis of RT-PCR in COVID-19 testing. The significant detection ability of this new optical-based platform demonstrated clinical potential.https://doi.org/10.1371/journal.pone.0254486 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pang-Yen Chen Cheng-Hao Ko C Jason Wang Chien-Wei Chen Wei-Huai Chiu Chitsung Hong Hao-Min Cheng I-Jen Wang |
spellingShingle |
Pang-Yen Chen Cheng-Hao Ko C Jason Wang Chien-Wei Chen Wei-Huai Chiu Chitsung Hong Hao-Min Cheng I-Jen Wang The early detection of immunoglobulins via optical-based lateral flow immunoassay platform in COVID-19 pandemic. PLoS ONE |
author_facet |
Pang-Yen Chen Cheng-Hao Ko C Jason Wang Chien-Wei Chen Wei-Huai Chiu Chitsung Hong Hao-Min Cheng I-Jen Wang |
author_sort |
Pang-Yen Chen |
title |
The early detection of immunoglobulins via optical-based lateral flow immunoassay platform in COVID-19 pandemic. |
title_short |
The early detection of immunoglobulins via optical-based lateral flow immunoassay platform in COVID-19 pandemic. |
title_full |
The early detection of immunoglobulins via optical-based lateral flow immunoassay platform in COVID-19 pandemic. |
title_fullStr |
The early detection of immunoglobulins via optical-based lateral flow immunoassay platform in COVID-19 pandemic. |
title_full_unstemmed |
The early detection of immunoglobulins via optical-based lateral flow immunoassay platform in COVID-19 pandemic. |
title_sort |
early detection of immunoglobulins via optical-based lateral flow immunoassay platform in covid-19 pandemic. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2021-01-01 |
description |
The coronavirus disease (COVID-19) is the global public health challenge currently persisting at a grand scale. A method that meets the rapid quantitative detection of antibodies to assess the body's immune response from natural COVID-19 illness or vaccines' effects is urgently needed. In the present study, an attempt was made to integrate a newly designed spectrometer to the COVID-19 test strip procedure; this augmentation provides the quantitative capacity to a lateral flow immunoassay (LFIA). Optical interpretation of results by quantitative α index, rather than visual qualification, can be done quickly, in 5-10 minutes. The developed product was compared with several other serological IgM/IgG antibody reagents on the market by recruiting 111 participants suspected of having COVID-19 infection from March to May 2020 in a hospital. Taking RT-PCR as the diagnostic gold standard, the quantitative spectral LIFA platform could correctly detect all 12 COVID-19 patients. Concerning RT-PCR negative patients, all three antibody testing methods found positive cases. The optical-based platform exhibited the ability of early detection of immunoglobulins of RT-PCR negative patients. There was an apparent trend that elevation of IgM levels in the acute phase of infection; then IgG levels rose later. It exhibited the risk of a false-negative diagnosis of RT-PCR in COVID-19 testing. The significant detection ability of this new optical-based platform demonstrated clinical potential. |
url |
https://doi.org/10.1371/journal.pone.0254486 |
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