Escherichia Coli Bl21: A Useful Biocatalyst for the Synthesis of Purine Nucleosides

E. coli BL21 cells were able to synthesize several purine nucleosides from pyrimidine ones. Kinetics and yields of this reaction showed a strong dependence on pH, temperature, reagent concentrations and weight of wet cell paste. Yields over 90% were reached in the synthesis of adenosine.

Bibliographic Details
Main Authors: A. Iribarren, E. Lewkowicz, S. Porro, N. Martínez, M. C. Rogert
Format: Article
Language:English
Published: MDPI AG 2000-03-01
Series:Molecules
Subjects:
n/a
Online Access:http://www.mdpi.com/1420-3049/5/3/535/