Qualitative and quantitative phytochemical composition, antimicrobial activity, and brine shrimp cytotoxicity of different solvent extracts of Acanthus polystachyus, Keetia gueinzii, and Rhynchosia elegans
Abstract Background The root, root bark, and root tubers of Acanthus polystachyus, Keetia gueinzii, and Rhynchosia elegans are used for managing bacterial and fungal infections among the Luo community of Kisumu East Sub County in Kenya. However, data on the efficacy of these plants against common ba...
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doaj-96f4bc2616b44d12819be0ebb39e0f462021-10-03T11:47:53ZengSpringerOpenFuture Journal of Pharmaceutical Sciences2314-72532021-09-017111110.1186/s43094-021-00342-zQualitative and quantitative phytochemical composition, antimicrobial activity, and brine shrimp cytotoxicity of different solvent extracts of Acanthus polystachyus, Keetia gueinzii, and Rhynchosia elegansJames Kiamba Mailu0Joseph Mwanzia Nguta1James Mucunu Mbaria2Mitchel Otieno Okumu3Department of Public Health, Pharmacology, and Toxicology, Faculty of Veterinary Medicine, University of NairobiDepartment of Public Health, Pharmacology, and Toxicology, Faculty of Veterinary Medicine, University of NairobiDepartment of Public Health, Pharmacology, and Toxicology, Faculty of Veterinary Medicine, University of NairobiDepartment of Public Health, Pharmacology, and Toxicology, Faculty of Veterinary Medicine, University of NairobiAbstract Background The root, root bark, and root tubers of Acanthus polystachyus, Keetia gueinzii, and Rhynchosia elegans are used for managing bacterial and fungal infections among the Luo community of Kisumu East Sub County in Kenya. However, data on the efficacy of these plants against common bacterial and fungal pathogens is not available. The safety of these plants is also not known. This study aimed to investigate the phytochemical composition, antimicrobial properties, and safety of different solvent extracts of the roots, root barks, and root tubers of Acanthus polystachyus, Keetia gueinzii, and Rhynchosia elegans. The broth microdilution method evaluated the antimicrobial activities of the root, root bark, and root tuber extracts (water, acetone, and methanol) of Acanthus polystachyus, Keetia gueinzii, and Rhynchosia elegans. Gram-positive (Bacillus cereus, Staphylococcus aureus), gram-negative (Escherichia coli), and fungal (Candida albicans) microorganisms were used in the evaluation. The safety of the extracts was evaluated in Artemia salina. The phytochemical composition of the extracts was determined using qualitative and quantitative assays. Results In general, the extracts of Acanthus polystachyus, Keetia gueinzii, and Rhynchosia elegans displayed poor antimicrobial properties relative to conventional antimicrobial agents including Amoxicillin, Gentamicin, and Nystatin. The aqueous extract of Acanthus polystachyus and the aqueous, acetone, and methanol extracts of Keetia gueinzii were safe in Artemia salina but all other extracts were cytotoxic to Artemia salina. Conclusions These findings suggest that the use of the roots, root barks, and root tubers of Acanthus polystachyus, Keetia gueinzii, and Rhynchosia elegans is limited by poor antimicrobial efficacy and cytotoxicity.https://doi.org/10.1186/s43094-021-00342-zAntimicrobial activityMedicinal plantsAcanthus polystachyusKeetia gueinziiRhynchosia elegansArtemia salina |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
James Kiamba Mailu Joseph Mwanzia Nguta James Mucunu Mbaria Mitchel Otieno Okumu |
spellingShingle |
James Kiamba Mailu Joseph Mwanzia Nguta James Mucunu Mbaria Mitchel Otieno Okumu Qualitative and quantitative phytochemical composition, antimicrobial activity, and brine shrimp cytotoxicity of different solvent extracts of Acanthus polystachyus, Keetia gueinzii, and Rhynchosia elegans Future Journal of Pharmaceutical Sciences Antimicrobial activity Medicinal plants Acanthus polystachyus Keetia gueinzii Rhynchosia elegans Artemia salina |
author_facet |
James Kiamba Mailu Joseph Mwanzia Nguta James Mucunu Mbaria Mitchel Otieno Okumu |
author_sort |
James Kiamba Mailu |
title |
Qualitative and quantitative phytochemical composition, antimicrobial activity, and brine shrimp cytotoxicity of different solvent extracts of Acanthus polystachyus, Keetia gueinzii, and Rhynchosia elegans |
title_short |
Qualitative and quantitative phytochemical composition, antimicrobial activity, and brine shrimp cytotoxicity of different solvent extracts of Acanthus polystachyus, Keetia gueinzii, and Rhynchosia elegans |
title_full |
Qualitative and quantitative phytochemical composition, antimicrobial activity, and brine shrimp cytotoxicity of different solvent extracts of Acanthus polystachyus, Keetia gueinzii, and Rhynchosia elegans |
title_fullStr |
Qualitative and quantitative phytochemical composition, antimicrobial activity, and brine shrimp cytotoxicity of different solvent extracts of Acanthus polystachyus, Keetia gueinzii, and Rhynchosia elegans |
title_full_unstemmed |
Qualitative and quantitative phytochemical composition, antimicrobial activity, and brine shrimp cytotoxicity of different solvent extracts of Acanthus polystachyus, Keetia gueinzii, and Rhynchosia elegans |
title_sort |
qualitative and quantitative phytochemical composition, antimicrobial activity, and brine shrimp cytotoxicity of different solvent extracts of acanthus polystachyus, keetia gueinzii, and rhynchosia elegans |
publisher |
SpringerOpen |
series |
Future Journal of Pharmaceutical Sciences |
issn |
2314-7253 |
publishDate |
2021-09-01 |
description |
Abstract Background The root, root bark, and root tubers of Acanthus polystachyus, Keetia gueinzii, and Rhynchosia elegans are used for managing bacterial and fungal infections among the Luo community of Kisumu East Sub County in Kenya. However, data on the efficacy of these plants against common bacterial and fungal pathogens is not available. The safety of these plants is also not known. This study aimed to investigate the phytochemical composition, antimicrobial properties, and safety of different solvent extracts of the roots, root barks, and root tubers of Acanthus polystachyus, Keetia gueinzii, and Rhynchosia elegans. The broth microdilution method evaluated the antimicrobial activities of the root, root bark, and root tuber extracts (water, acetone, and methanol) of Acanthus polystachyus, Keetia gueinzii, and Rhynchosia elegans. Gram-positive (Bacillus cereus, Staphylococcus aureus), gram-negative (Escherichia coli), and fungal (Candida albicans) microorganisms were used in the evaluation. The safety of the extracts was evaluated in Artemia salina. The phytochemical composition of the extracts was determined using qualitative and quantitative assays. Results In general, the extracts of Acanthus polystachyus, Keetia gueinzii, and Rhynchosia elegans displayed poor antimicrobial properties relative to conventional antimicrobial agents including Amoxicillin, Gentamicin, and Nystatin. The aqueous extract of Acanthus polystachyus and the aqueous, acetone, and methanol extracts of Keetia gueinzii were safe in Artemia salina but all other extracts were cytotoxic to Artemia salina. Conclusions These findings suggest that the use of the roots, root barks, and root tubers of Acanthus polystachyus, Keetia gueinzii, and Rhynchosia elegans is limited by poor antimicrobial efficacy and cytotoxicity. |
topic |
Antimicrobial activity Medicinal plants Acanthus polystachyus Keetia gueinzii Rhynchosia elegans Artemia salina |
url |
https://doi.org/10.1186/s43094-021-00342-z |
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