Inhibitory Effects of Vaccinium arctostaphylos Extract on Prostate Cancer Cells

Background & objectives: Prostate cancer is one of the most common cancers in the world, and its prevention is expected to increase in the future appreciably. Following the emergence of the new field of nutritional genetics and investigations about the effect of natural antioxidants on the hyper...

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Main Authors: Elmira Gorbanzadeh, Mohammad Zaefizadeh
Format: Article
Language:fas
Published: Ardabil University of Medical Sciences 2018-01-01
Series:Journal of Ardabil University of Medical Sciences
Subjects:
Online Access:http://jarums.arums.ac.ir/browse.php?a_code=A-10-607-89&slc_lang=en&sid=1
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spelling doaj-983d7706235d4f8ab52e9fbcbb2622912020-11-25T03:36:04ZfasArdabil University of Medical SciencesJournal of Ardabil University of Medical Sciences2228-72802228-72992018-01-01174487496Inhibitory Effects of Vaccinium arctostaphylos Extract on Prostate Cancer CellsElmira Gorbanzadeh0Mohammad Zaefizadeh1 Department of Biology, School of Sciences, Ahar branch, Islamic Azad University, Ahar, Iran Department of Biology, School of Sciences, Ardabil branch, Islamic Azad University, Ardabil, Iran Background & objectives: Prostate cancer is one of the most common cancers in the world, and its prevention is expected to increase in the future appreciably. Following the emergence of the new field of nutritional genetics and investigations about the effect of natural antioxidants on the hypermethylation of genes, this study was performed to measure the effect of  Vaccinium arctostaphylos poly phenols and anthocyanins on PC-3 cell line as well as methylation and expression changes of GSTP1 gene. Methods:  In order to evaluate the survival potency of PC-3 cell line in a completely randomized design, MTT test was used and these cells were treated with different concentrations of Vaccinium arctostaphylos extract (4500, 2250, 1125, 562.5, 140.62, 70.31, 35.15, 17.57, 8.78, 4.39 µg/ml) for 24, 48 and 72 hours. Expression level of GSTP1 gene was analyzed by Q-RT-PCR method in 2500, 1250, 625, 312 and 156 µg/ml concentrations of Vaccinium. Results: Statistical results showed that Vaccinium arctostaphylos poly phenolic extract at 35-70 ppm concentration  significantly reduced the survival rate of  PC-3 cells. Moreover, compared to control cells, the expression level of GST gene significantly increased in PC-3 cells treated with 1250ppm extract. Conclusion: Anthocyanin- polyphenolic extract from Vaccinium arctostaphylos can decrease the survival rate of cancerous cells and GST gene expression in human prostate cancer PC-3 cells. This may be explained by changes in cell carcinogenesis pathway or CpG demethylation process. Keywords: Vaccinium arctostaphylos; Antioxidant; Cancerous Cells; Prostate.   Background & objectives: Prostate cancer is one of the most common cancers in the world, and its prevention is expected to increase in the future appreciably. Following the emergence of the new field of nutritional genetics and investigations about the effect of natural antioxidants on the hypermethylation of genes, this study was performed to measure the effect of  Vaccinium arctostaphylos poly phenols and anthocyanins on PC-3 cell line as well as methylation and expression changes of GSTP1 gene. Methods:  In order to evaluate the survival potency of PC-3 cell line in a completely randomized design, MTT test was used and these cells were treated with different concentrations of Vaccinium arctostaphylos extract (4500, 2250, 1125, 562.5, 140.62, 70.31, 35.15, 17.57, 8.78, 4.39 µg/ml) for 24, 48 and 72 hours. Expression level of GSTP1 gene was analyzed by Q-RT-PCR method in 2500, 1250, 625, 312 and 156 µg/ml concentrations of Vaccinium. Results: Statistical results showed that Vaccinium arctostaphylos poly phenolic extract at 35-70 ppm concentration  significantly reduced the survival rate of  PC-3 cells. Moreover, compared to control cells, the expression level of GST gene significantly increased in PC-3 cells treated with 1250ppm extract. Conclusion: Anthocyanin- polyphenolic extract from Vaccinium arctostaphylos can decrease the survival rate of cancerous cells and GST gene expression in human prostate cancer PC-3 cells. This may be explained by changes in cell carcinogenesis pathway or CpG demethylation process.           http://jarums.arums.ac.ir/browse.php?a_code=A-10-607-89&slc_lang=en&sid=1 Vaccinium arctostaphylos Antioxidant Cancerous Cells Prostate.
collection DOAJ
language fas
format Article
sources DOAJ
author Elmira Gorbanzadeh
Mohammad Zaefizadeh
spellingShingle Elmira Gorbanzadeh
Mohammad Zaefizadeh
Inhibitory Effects of Vaccinium arctostaphylos Extract on Prostate Cancer Cells
Journal of Ardabil University of Medical Sciences
Vaccinium arctostaphylos
Antioxidant
Cancerous Cells
Prostate.
author_facet Elmira Gorbanzadeh
Mohammad Zaefizadeh
author_sort Elmira Gorbanzadeh
title Inhibitory Effects of Vaccinium arctostaphylos Extract on Prostate Cancer Cells
title_short Inhibitory Effects of Vaccinium arctostaphylos Extract on Prostate Cancer Cells
title_full Inhibitory Effects of Vaccinium arctostaphylos Extract on Prostate Cancer Cells
title_fullStr Inhibitory Effects of Vaccinium arctostaphylos Extract on Prostate Cancer Cells
title_full_unstemmed Inhibitory Effects of Vaccinium arctostaphylos Extract on Prostate Cancer Cells
title_sort inhibitory effects of vaccinium arctostaphylos extract on prostate cancer cells
publisher Ardabil University of Medical Sciences
series Journal of Ardabil University of Medical Sciences
issn 2228-7280
2228-7299
publishDate 2018-01-01
description Background & objectives: Prostate cancer is one of the most common cancers in the world, and its prevention is expected to increase in the future appreciably. Following the emergence of the new field of nutritional genetics and investigations about the effect of natural antioxidants on the hypermethylation of genes, this study was performed to measure the effect of  Vaccinium arctostaphylos poly phenols and anthocyanins on PC-3 cell line as well as methylation and expression changes of GSTP1 gene. Methods:  In order to evaluate the survival potency of PC-3 cell line in a completely randomized design, MTT test was used and these cells were treated with different concentrations of Vaccinium arctostaphylos extract (4500, 2250, 1125, 562.5, 140.62, 70.31, 35.15, 17.57, 8.78, 4.39 µg/ml) for 24, 48 and 72 hours. Expression level of GSTP1 gene was analyzed by Q-RT-PCR method in 2500, 1250, 625, 312 and 156 µg/ml concentrations of Vaccinium. Results: Statistical results showed that Vaccinium arctostaphylos poly phenolic extract at 35-70 ppm concentration  significantly reduced the survival rate of  PC-3 cells. Moreover, compared to control cells, the expression level of GST gene significantly increased in PC-3 cells treated with 1250ppm extract. Conclusion: Anthocyanin- polyphenolic extract from Vaccinium arctostaphylos can decrease the survival rate of cancerous cells and GST gene expression in human prostate cancer PC-3 cells. This may be explained by changes in cell carcinogenesis pathway or CpG demethylation process. Keywords: Vaccinium arctostaphylos; Antioxidant; Cancerous Cells; Prostate.   Background & objectives: Prostate cancer is one of the most common cancers in the world, and its prevention is expected to increase in the future appreciably. Following the emergence of the new field of nutritional genetics and investigations about the effect of natural antioxidants on the hypermethylation of genes, this study was performed to measure the effect of  Vaccinium arctostaphylos poly phenols and anthocyanins on PC-3 cell line as well as methylation and expression changes of GSTP1 gene. Methods:  In order to evaluate the survival potency of PC-3 cell line in a completely randomized design, MTT test was used and these cells were treated with different concentrations of Vaccinium arctostaphylos extract (4500, 2250, 1125, 562.5, 140.62, 70.31, 35.15, 17.57, 8.78, 4.39 µg/ml) for 24, 48 and 72 hours. Expression level of GSTP1 gene was analyzed by Q-RT-PCR method in 2500, 1250, 625, 312 and 156 µg/ml concentrations of Vaccinium. Results: Statistical results showed that Vaccinium arctostaphylos poly phenolic extract at 35-70 ppm concentration  significantly reduced the survival rate of  PC-3 cells. Moreover, compared to control cells, the expression level of GST gene significantly increased in PC-3 cells treated with 1250ppm extract. Conclusion: Anthocyanin- polyphenolic extract from Vaccinium arctostaphylos can decrease the survival rate of cancerous cells and GST gene expression in human prostate cancer PC-3 cells. This may be explained by changes in cell carcinogenesis pathway or CpG demethylation process.           
topic Vaccinium arctostaphylos
Antioxidant
Cancerous Cells
Prostate.
url http://jarums.arums.ac.ir/browse.php?a_code=A-10-607-89&slc_lang=en&sid=1
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