Therapeutic Potential of Human Bone Marrow Derived Mesenchymal Stromal Cells in Combination with Silymarin against Carbon Tetrachloride Induced Liver Cirrhosis in Wistar Rats
Background: Self-renewal, active proliferation in vitro, abundant sources for isolation, and a high differentiation capacity, make mesenchymal stem cells to be potentially therapeutic for liver cirrhosis. Aim and Objectives: To evaluate the therapeutic potential of the combination of human Bone...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Krishna Institute of Medical Sciences University
2017-10-01
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| Series: | Journal of Krishna Institute of Medical Sciences University |
| Subjects: | |
| Online Access: | http://jkimsu.com/jkimsu-vol6no4/JKIMSU,%20Vol.%206,%20No.%204,%20October-December%202017%20Page%2038-49.pdf |
| Summary: | Background: Self-renewal, active proliferation in
vitro, abundant sources for isolation, and a high
differentiation capacity, make mesenchymal stem cells
to be potentially therapeutic for liver cirrhosis. Aim
and Objectives: To evaluate the therapeutic potential
of the combination of human Bone Marrow derived
Mesenchymal Stromal Cells (BM-MSC) and
silymarin in Carbon tetrachloride (CCl ) induced liver 4
cirrhosis in Wistar rats. Material and Methods: This
was an experimental study. Liver cirrhosis was
induced in adult female Wistar rats using CCl . Sixty 4
rats were randomly divided into 6 groups: Group 1
(Normal Control Group), Group 2 (received only
CCl ), Group 3 (CCl +low dose BM-MSCs), Group 4 4 4
(CCl4+high dose BM-MSCs), Group 5 (CCl4+
silymarin), Group 6 (CCl +BM-MSCs+silymarin). On 4
th th th th th day 0 and at the end of 6 , 12 , 18 , 24 and 30 day
after treatment, blood samples were collected for liver
enzyme estimations. After 30 days of treatment, the
rats were sacrificed; livers were excised and used for
antioxidant analysis and histopathological studies.
Results: Liver enzyme analysis and histopathological
studies indicated that combination of BM-MSCs and
silymarin was effective in treating liver cirrhosis.
Furthermore, oxidative stress was attenuated in the
group which received combination treatment of BMMSCs
and silymarin. Conclusion: Evidence of this
study showed that combination treatment of BM-MSCs and silymarin was beneficial and support the
potential of using MSCs transplantation as an effective
treatment modality for liver cirrhosis |
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| ISSN: | 2231-4261 2231-4261 |