Therapeutic Potential of Human Bone Marrow Derived Mesenchymal Stromal Cells in Combination with Silymarin against Carbon Tetrachloride Induced Liver Cirrhosis in Wistar Rats

• Main Authors: Ashwini P. Aithal, Laxminarayana K. Bairy, Raviraja N. Seetharam, Naveen Kumar
• Format: Article
• Language: English
• Published: Krishna Institute of Medical Sciences University 2017-10-01
• Series: Journal of Krishna Institute of Medical Sciences University
• Subjects: Liver Cirrhosis; Mesenchymal Stromal Cells; Transplantation; Carbon tetrachloride; Silymarin
• Online Access: http://jkimsu.com/jkimsu-vol6no4/JKIMSU,%20Vol.%206,%20No.%204,%20October-December%202017%20Page%2038-49.pdf

Background: Self-renewal, active proliferation in vitro, abundant sources for isolation, and a high differentiation capacity, make mesenchymal stem cells to be potentially therapeutic for liver cirrhosis. Aim and Objectives: To evaluate the therapeutic potential of the combination of human Bone Marrow derived Mesenchymal Stromal Cells (BM-MSC) and silymarin in Carbon tetrachloride (CCl ) induced liver 4 cirrhosis in Wistar rats. Material and Methods: This was an experimental study. Liver cirrhosis was induced in adult female Wistar rats using CCl . Sixty 4 rats were randomly divided into 6 groups: Group 1 (Normal Control Group), Group 2 (received only CCl ), Group 3 (CCl +low dose BM-MSCs), Group 4 4 4 (CCl4+high dose BM-MSCs), Group 5 (CCl4+ silymarin), Group 6 (CCl +BM-MSCs+silymarin). On 4 th th th th th day 0 and at the end of 6 , 12 , 18 , 24 and 30 day after treatment, blood samples were collected for liver enzyme estimations. After 30 days of treatment, the rats were sacrificed; livers were excised and used for antioxidant analysis and histopathological studies. Results: Liver enzyme analysis and histopathological studies indicated that combination of BM-MSCs and silymarin was effective in treating liver cirrhosis. Furthermore, oxidative stress was attenuated in the group which received combination treatment of BMMSCs and silymarin. Conclusion: Evidence of this study showed that combination treatment of BM-MSCs and silymarin was beneficial and support the potential of using MSCs transplantation as an effective treatment modality for liver cirrhosis