Heterologous expression of genes for bioconversion of xylose to xylonic acid in Corynebacterium glutamicum and optimization of the bioprocess
Abstract In bacterial system, direct conversion of xylose to xylonic acid is mediated through NAD-dependent xylose dehydrogenase (xylB) and xylonolactonase (xylC) genes. Heterologous expression of these genes from Caulobacter crescentus into recombinant Corynebacterium glutamicum ATCC 13032 and C. g...
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doaj-9a50bcd12ff84cc3add71e80870355ef2020-11-25T02:01:14ZengSpringerOpenAMB Express2191-08552020-04-0110111110.1186/s13568-020-01003-9Heterologous expression of genes for bioconversion of xylose to xylonic acid in Corynebacterium glutamicum and optimization of the bioprocessM. S. Lekshmi Sundar0Aliyath Susmitha1Devi Rajan2Silvin Hannibal3Keerthi Sasikumar4Volker F. Wendisch5K. Madhavan Nampoothiri6Microbial Processes and Technology Division, CSIR–National Institute for Interdisciplinary Science and Technology (NIIST)Microbial Processes and Technology Division, CSIR–National Institute for Interdisciplinary Science and Technology (NIIST)Microbial Processes and Technology Division, CSIR–National Institute for Interdisciplinary Science and Technology (NIIST)Genetics of Prokaryotes, Faculty of Biology & CeBiTec, Bielefeld UniversityMicrobial Processes and Technology Division, CSIR–National Institute for Interdisciplinary Science and Technology (NIIST)Genetics of Prokaryotes, Faculty of Biology & CeBiTec, Bielefeld UniversityMicrobial Processes and Technology Division, CSIR–National Institute for Interdisciplinary Science and Technology (NIIST)Abstract In bacterial system, direct conversion of xylose to xylonic acid is mediated through NAD-dependent xylose dehydrogenase (xylB) and xylonolactonase (xylC) genes. Heterologous expression of these genes from Caulobacter crescentus into recombinant Corynebacterium glutamicum ATCC 13032 and C. glutamicum ATCC 31831 (with an innate pentose transporter, araE) resulted in an efficient bioconversion process to produce xylonic acid from xylose. Process parameters including the design of production medium was optimized using a statistical tool, Response Surface Methodology (RSM). Maximum xylonic acid of 56.32 g/L from 60 g/L xylose, i.e. about 76.67% of the maximum theoretical yield was obtained after 120 h fermentation from pure xylose with recombinant C. glutamicum ATCC 31831 containing the plasmid pVWEx1 xylB. Under the same condition, the production with recombinant C. glutamicum ATCC 13032 (with pVWEx1 xylB) was 50.66 g/L, i.e. 69% of the theoretical yield. There was no significant improvement in production with the simultaneous expression of xylB and xylC genes together indicating xylose dehydrogenase activity as one of the rate limiting factor in the bioconversion. Finally, proof of concept experiment in utilizing biomass derived pentose sugar, xylose, for xylonic acid production was also carried out and obtained 42.94 g/L xylonic acid from 60 g/L xylose. These results promise a significant value addition for the future bio refinery programs.http://link.springer.com/article/10.1186/s13568-020-01003-9Corynebacterium glutamicumBiomassHeterologous expressionResponse surface methodology (RSM)XyloseXylonic acid |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
M. S. Lekshmi Sundar Aliyath Susmitha Devi Rajan Silvin Hannibal Keerthi Sasikumar Volker F. Wendisch K. Madhavan Nampoothiri |
spellingShingle |
M. S. Lekshmi Sundar Aliyath Susmitha Devi Rajan Silvin Hannibal Keerthi Sasikumar Volker F. Wendisch K. Madhavan Nampoothiri Heterologous expression of genes for bioconversion of xylose to xylonic acid in Corynebacterium glutamicum and optimization of the bioprocess AMB Express Corynebacterium glutamicum Biomass Heterologous expression Response surface methodology (RSM) Xylose Xylonic acid |
author_facet |
M. S. Lekshmi Sundar Aliyath Susmitha Devi Rajan Silvin Hannibal Keerthi Sasikumar Volker F. Wendisch K. Madhavan Nampoothiri |
author_sort |
M. S. Lekshmi Sundar |
title |
Heterologous expression of genes for bioconversion of xylose to xylonic acid in Corynebacterium glutamicum and optimization of the bioprocess |
title_short |
Heterologous expression of genes for bioconversion of xylose to xylonic acid in Corynebacterium glutamicum and optimization of the bioprocess |
title_full |
Heterologous expression of genes for bioconversion of xylose to xylonic acid in Corynebacterium glutamicum and optimization of the bioprocess |
title_fullStr |
Heterologous expression of genes for bioconversion of xylose to xylonic acid in Corynebacterium glutamicum and optimization of the bioprocess |
title_full_unstemmed |
Heterologous expression of genes for bioconversion of xylose to xylonic acid in Corynebacterium glutamicum and optimization of the bioprocess |
title_sort |
heterologous expression of genes for bioconversion of xylose to xylonic acid in corynebacterium glutamicum and optimization of the bioprocess |
publisher |
SpringerOpen |
series |
AMB Express |
issn |
2191-0855 |
publishDate |
2020-04-01 |
description |
Abstract In bacterial system, direct conversion of xylose to xylonic acid is mediated through NAD-dependent xylose dehydrogenase (xylB) and xylonolactonase (xylC) genes. Heterologous expression of these genes from Caulobacter crescentus into recombinant Corynebacterium glutamicum ATCC 13032 and C. glutamicum ATCC 31831 (with an innate pentose transporter, araE) resulted in an efficient bioconversion process to produce xylonic acid from xylose. Process parameters including the design of production medium was optimized using a statistical tool, Response Surface Methodology (RSM). Maximum xylonic acid of 56.32 g/L from 60 g/L xylose, i.e. about 76.67% of the maximum theoretical yield was obtained after 120 h fermentation from pure xylose with recombinant C. glutamicum ATCC 31831 containing the plasmid pVWEx1 xylB. Under the same condition, the production with recombinant C. glutamicum ATCC 13032 (with pVWEx1 xylB) was 50.66 g/L, i.e. 69% of the theoretical yield. There was no significant improvement in production with the simultaneous expression of xylB and xylC genes together indicating xylose dehydrogenase activity as one of the rate limiting factor in the bioconversion. Finally, proof of concept experiment in utilizing biomass derived pentose sugar, xylose, for xylonic acid production was also carried out and obtained 42.94 g/L xylonic acid from 60 g/L xylose. These results promise a significant value addition for the future bio refinery programs. |
topic |
Corynebacterium glutamicum Biomass Heterologous expression Response surface methodology (RSM) Xylose Xylonic acid |
url |
http://link.springer.com/article/10.1186/s13568-020-01003-9 |
work_keys_str_mv |
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