The influence of a cryoprotective medium containing glycerol on the lyophilization of lactic acid bacteria
The aims of liophilization (freeze-drying) of lactic acid bacteria are to preserve pure cultures or to prepare starters for the dairy industry. In both cases, the choice of the cryoprotectant is very important. In this work, samples of Bifidobacterium breve A71 and Bifidobacterium bifidum B...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Serbian Chemical Society
2001-01-01
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Series: | Journal of the Serbian Chemical Society |
Subjects: | |
Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2001/0352-51390107435T.pdf |
Summary: | The aims of liophilization (freeze-drying) of lactic acid bacteria are to
preserve pure cultures or to prepare starters for the dairy industry. In
both cases, the choice of the cryoprotectant is very important. In this
work, samples of Bifidobacterium breve A71 and Bifidobacterium bifidum BbTD
were freeze-dried in a new cryoprotective medium containing lactose,
gelatine and glycerol (medium B). The reference medium contained saccharose,
gelatine and skim milk (medium A). Before liophilization, the eutectic
points of both media were determined, because the products must be cooled to
a temperature below its freezing point. The success of the cryoprotectants
was estimated in terms of the number of surviving organisms after
lyophilization. Bifidobacterium breve A71 and Bifidobacterium bifidum BbTD
freeze-dried in mediaAandBshowed high survival rates. Bifidobacterium breve
A71 showed a greater percentage survival in combination with medium B than
with medium A. These results could be utilized in the manufacture of
Bifidobacterium breve A71 as a starter in the diary industry because it is a
human isolate which, except for acidification, has probiotic activity. |
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ISSN: | 0352-5139 1820-7421 |