Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9

Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9

CRISPR-Cas9 has been used to image repetitive loci in live cells to explore genome organization. Here the authors demonstrate the imaging of low-repeat regions with a single-guide RNA and the imaging of a non-repetitive region, allowing the tracking of transcriptionally active and inactive regions.

Bibliographic Details
Main Authors: Peiwu Qin, Mahmut Parlak, Cem Kuscu, Jigar Bandaria, Mustafa Mir, Karol Szlachta, Ritambhara Singh, Xavier Darzacq, Ahmet Yildiz, Mazhar Adli
Format: Article
Language:English
Published: Nature Publishing Group 2017-03-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/ncomms14725

Internet

https://doi.org/10.1038/ncomms14725

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