Functional Characterization of Cryptococcal Genes: Then and Now

Functional Characterization of Cryptococcal Genes: Then and Now

Site-directed mutagenesis enables researchers to switch a gene of interest off for functional characterization of the gene. In the pathogenic yeasts, Cryptococcus neoformans and sister species C. deneoformans, this is almost exclusively achieved by introducing DNA into cells through either biolistic...

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Bibliographic Details
Main Authors: Lukas M. du Plooy, Olihile M. Sebolai, Carolina H. Pohl, Jacobus Albertyn
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-09-01
Series:Frontiers in Microbiology
Subjects:
biolistic transformation
CRISPR-Cas9
Cryptococcus
electroporation
gene targeting
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2018.02263/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2018.02263/full

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