The effect of L-Arginine on the brain tissue of stressed rats
Abstract Introduction: This study was conducted to determine the possible beneficial results of L-arginine on prefrontal cortex of rats which impressed by immobilization stress to define the synchronous impression of stress and nitric oxide (NO) on evolution of prefrontal cortex of rats after bir...
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Iran University of Medical Sciences
2010-01-01
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doaj-a00b6150647545b6aee58915f665708b2020-11-24T22:46:17ZengIran University of Medical SciencesBasic and Clinical Neuroscience2008-126X2228-74422010-01-01123336The effect of L-Arginine on the brain tissue of stressed ratsBatoul EbadiMehdi MehdizadehArezoo NahavandiTabandeh ShariatiHamdollah Delaviz Abstract Introduction: This study was conducted to determine the possible beneficial results of L-arginine on prefrontal cortex of rats which impressed by immobilization stress to define the synchronous impression of stress and nitric oxide (NO) on evolution of prefrontal cortex of rats after birth. Methods: Forty-eight one month, male Wistar rats were divided into two groups: stressed and non-stressed. L-Arginine (200 mg/kg) as a NO synthase (NOS) inducer and L-NAME (2O mg/kg) were injected intraperitonealy (IP) and 7- nitroindazde (25 mg/kg) as non-specific was injected subcutaneously (S.C.) for 4 weeks. The kind of stress was immobilization for 4 weeks, every other day. The brain was removed after this period and each brain divided into two parts in a coronal section manner. Anterior part used for histological studies with H&E staining and posterior part used for measurement of NO production using spectrophotometer at 540 nm wavelengh. Results: Statistical analysis of microscopic and light microscopic finding showed that thickness of prefrontal cortex and NO production were significantly decreased in stressed rats and especially in groups which received 7- nitroindazole and L-NAME and L-arginine could reverse these results. Discussion: According to this research, we could say that L-arginine decreases the cortical damages in stressed rats and 7-nitroindazole and L-NAME increase this damage in non-stressed group. Although in non stressed groups, L-arginine, L-NAME and 7- nitroindazole were all non-protective and damaging.http://bcn.tums.ac.ir/browse.php?a_code=A-10-1-24&slc_lang=en&sid=1Nitric oxideImmobilization stressPrefrontal cortexL-NAME7- nitroindazole |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Batoul Ebadi Mehdi Mehdizadeh Arezoo Nahavandi Tabandeh Shariati Hamdollah Delaviz |
spellingShingle |
Batoul Ebadi Mehdi Mehdizadeh Arezoo Nahavandi Tabandeh Shariati Hamdollah Delaviz The effect of L-Arginine on the brain tissue of stressed rats Basic and Clinical Neuroscience Nitric oxide Immobilization stress Prefrontal cortex L-NAME 7- nitroindazole |
author_facet |
Batoul Ebadi Mehdi Mehdizadeh Arezoo Nahavandi Tabandeh Shariati Hamdollah Delaviz |
author_sort |
Batoul Ebadi |
title |
The effect of L-Arginine on the brain tissue of stressed rats |
title_short |
The effect of L-Arginine on the brain tissue of stressed rats |
title_full |
The effect of L-Arginine on the brain tissue of stressed rats |
title_fullStr |
The effect of L-Arginine on the brain tissue of stressed rats |
title_full_unstemmed |
The effect of L-Arginine on the brain tissue of stressed rats |
title_sort |
effect of l-arginine on the brain tissue of stressed rats |
publisher |
Iran University of Medical Sciences |
series |
Basic and Clinical Neuroscience |
issn |
2008-126X 2228-7442 |
publishDate |
2010-01-01 |
description |
Abstract Introduction: This study was conducted to determine the possible beneficial results of L-arginine on prefrontal cortex of rats which impressed by immobilization stress to define the synchronous impression of stress and nitric oxide (NO) on evolution of prefrontal cortex of rats after birth. Methods: Forty-eight one month, male Wistar rats were divided into two groups: stressed and non-stressed. L-Arginine (200 mg/kg) as a NO synthase (NOS) inducer and L-NAME (2O mg/kg) were injected intraperitonealy (IP) and 7- nitroindazde (25 mg/kg) as non-specific was injected subcutaneously (S.C.) for 4 weeks. The kind of stress was immobilization for 4 weeks, every other day. The brain was removed after this period and each brain divided into two parts in a coronal section manner. Anterior part used for histological studies with H&E staining and posterior part used for measurement of NO production using spectrophotometer at 540 nm wavelengh. Results: Statistical analysis of microscopic and light microscopic finding showed that thickness of prefrontal cortex and NO production were significantly decreased in stressed rats and especially in groups which received 7- nitroindazole and L-NAME and L-arginine could reverse these results. Discussion: According to this research, we could say that L-arginine decreases the cortical damages in stressed rats and 7-nitroindazole and L-NAME increase this damage in non-stressed group. Although in non stressed groups, L-arginine, L-NAME and 7- nitroindazole were all non-protective and damaging. |
topic |
Nitric oxide Immobilization stress Prefrontal cortex L-NAME 7- nitroindazole |
url |
http://bcn.tums.ac.ir/browse.php?a_code=A-10-1-24&slc_lang=en&sid=1 |
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