Inhibition effects of 7-phloro-eckol from Ecklonia cava on metastasis and angiogenesis induced by hypoxia through regulation of AKT/mTOR and ERK signaling pathways

As one of the malignant tumors with high mortality, liver cancer has the characteristics of early invasion and metastasis, and lacks effective treatment methods. Therefore, it is urgent to find new safe, efficient and low-toxic anti-liver cancer drugs. In this study, the evaluated mechanism of Ecklo...

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Main Authors: Shengtao Yang, Yi Liu, Zhenbang Xiao, Yanfei Tang, Pengzhi Hong, Shengli Sun, Chunxia Zhou, Zhong-Ji Qian
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:Arabian Journal of Chemistry
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1878535221002021
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spelling doaj-a0a34b1e3f2347d99bb77fbcfcfbe8112021-05-28T05:01:02ZengElsevierArabian Journal of Chemistry1878-53522021-06-01146103187Inhibition effects of 7-phloro-eckol from Ecklonia cava on metastasis and angiogenesis induced by hypoxia through regulation of AKT/mTOR and ERK signaling pathwaysShengtao Yang0Yi Liu1Zhenbang Xiao2Yanfei Tang3Pengzhi Hong4Shengli Sun5Chunxia Zhou6Zhong-Ji Qian7College of Food Science and Technology, School of Chemistry and Environment, Shenzhen Institute of Guangdong Ocean University, Guangdong Ocean University, Zhanjiang 524088, People’s Republic of China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524025, People’s Republic of ChinaCollege of Food Science and Technology, School of Chemistry and Environment, Shenzhen Institute of Guangdong Ocean University, Guangdong Ocean University, Zhanjiang 524088, People’s Republic of ChinaCollege of Food Science and Technology, School of Chemistry and Environment, Shenzhen Institute of Guangdong Ocean University, Guangdong Ocean University, Zhanjiang 524088, People’s Republic of China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524025, People’s Republic of ChinaCollege of Food Science and Technology, School of Chemistry and Environment, Shenzhen Institute of Guangdong Ocean University, Guangdong Ocean University, Zhanjiang 524088, People’s Republic of ChinaCollege of Food Science and Technology, School of Chemistry and Environment, Shenzhen Institute of Guangdong Ocean University, Guangdong Ocean University, Zhanjiang 524088, People’s Republic of China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524025, People’s Republic of ChinaCollege of Food Science and Technology, School of Chemistry and Environment, Shenzhen Institute of Guangdong Ocean University, Guangdong Ocean University, Zhanjiang 524088, People’s Republic of ChinaCollege of Food Science and Technology, School of Chemistry and Environment, Shenzhen Institute of Guangdong Ocean University, Guangdong Ocean University, Zhanjiang 524088, People’s Republic of China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524025, People’s Republic of China; Corresponding authors.College of Food Science and Technology, School of Chemistry and Environment, Shenzhen Institute of Guangdong Ocean University, Guangdong Ocean University, Zhanjiang 524088, People’s Republic of China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524025, People’s Republic of China; Corresponding authors.As one of the malignant tumors with high mortality, liver cancer has the characteristics of early invasion and metastasis, and lacks effective treatment methods. Therefore, it is urgent to find new safe, efficient and low-toxic anti-liver cancer drugs. In this study, the evaluated mechanism of Ecklonia cava polyphenols (7PE, 7-phloro-eckol) as a potential active substance for inhibiting liver cancer metastasis (HepG2 cell) and angiogenesis (HUVEC cell). The results have shown that 7PE can inhibit the expression of hypoxia-inducible factors (HIF-1α) protein by regulating the PI3K/AKT/mTOR and Ras/MEK/ERK/MNK signaling pathways under hypoxic conditions in HepG2 cells, thereby blocking the secretion of vascular growth factor (VEGF) protein. At the same time, 7PE can significantly inhibit the conduction of AKT and MAPK signaling pathways in HUVEC by suppressing the activation of VEGFR-2 protein, which verifies the prediction of molecular docking. Therefore, the results of this study provide a research basis for 7PE to inhibit metastasis and angiogenesis of liver cancer cells, and provide a theoretical basis for the high-value utilization of Ecklonia cava.http://www.sciencedirect.com/science/article/pii/S18785352210020217-phloro-eckolEcklonia cavaHepatomaHypoxiaMetastasisAngiogenesis
collection DOAJ
language English
format Article
sources DOAJ
author Shengtao Yang
Yi Liu
Zhenbang Xiao
Yanfei Tang
Pengzhi Hong
Shengli Sun
Chunxia Zhou
Zhong-Ji Qian
spellingShingle Shengtao Yang
Yi Liu
Zhenbang Xiao
Yanfei Tang
Pengzhi Hong
Shengli Sun
Chunxia Zhou
Zhong-Ji Qian
Inhibition effects of 7-phloro-eckol from Ecklonia cava on metastasis and angiogenesis induced by hypoxia through regulation of AKT/mTOR and ERK signaling pathways
Arabian Journal of Chemistry
7-phloro-eckol
Ecklonia cava
Hepatoma
Hypoxia
Metastasis
Angiogenesis
author_facet Shengtao Yang
Yi Liu
Zhenbang Xiao
Yanfei Tang
Pengzhi Hong
Shengli Sun
Chunxia Zhou
Zhong-Ji Qian
author_sort Shengtao Yang
title Inhibition effects of 7-phloro-eckol from Ecklonia cava on metastasis and angiogenesis induced by hypoxia through regulation of AKT/mTOR and ERK signaling pathways
title_short Inhibition effects of 7-phloro-eckol from Ecklonia cava on metastasis and angiogenesis induced by hypoxia through regulation of AKT/mTOR and ERK signaling pathways
title_full Inhibition effects of 7-phloro-eckol from Ecklonia cava on metastasis and angiogenesis induced by hypoxia through regulation of AKT/mTOR and ERK signaling pathways
title_fullStr Inhibition effects of 7-phloro-eckol from Ecklonia cava on metastasis and angiogenesis induced by hypoxia through regulation of AKT/mTOR and ERK signaling pathways
title_full_unstemmed Inhibition effects of 7-phloro-eckol from Ecklonia cava on metastasis and angiogenesis induced by hypoxia through regulation of AKT/mTOR and ERK signaling pathways
title_sort inhibition effects of 7-phloro-eckol from ecklonia cava on metastasis and angiogenesis induced by hypoxia through regulation of akt/mtor and erk signaling pathways
publisher Elsevier
series Arabian Journal of Chemistry
issn 1878-5352
publishDate 2021-06-01
description As one of the malignant tumors with high mortality, liver cancer has the characteristics of early invasion and metastasis, and lacks effective treatment methods. Therefore, it is urgent to find new safe, efficient and low-toxic anti-liver cancer drugs. In this study, the evaluated mechanism of Ecklonia cava polyphenols (7PE, 7-phloro-eckol) as a potential active substance for inhibiting liver cancer metastasis (HepG2 cell) and angiogenesis (HUVEC cell). The results have shown that 7PE can inhibit the expression of hypoxia-inducible factors (HIF-1α) protein by regulating the PI3K/AKT/mTOR and Ras/MEK/ERK/MNK signaling pathways under hypoxic conditions in HepG2 cells, thereby blocking the secretion of vascular growth factor (VEGF) protein. At the same time, 7PE can significantly inhibit the conduction of AKT and MAPK signaling pathways in HUVEC by suppressing the activation of VEGFR-2 protein, which verifies the prediction of molecular docking. Therefore, the results of this study provide a research basis for 7PE to inhibit metastasis and angiogenesis of liver cancer cells, and provide a theoretical basis for the high-value utilization of Ecklonia cava.
topic 7-phloro-eckol
Ecklonia cava
Hepatoma
Hypoxia
Metastasis
Angiogenesis
url http://www.sciencedirect.com/science/article/pii/S1878535221002021
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