Detection of Epstein–Barr Virus in Periodontitis: A Review of Methodological Approaches
Periodontitis, an inflammatory condition that affects the structures surrounding the tooth eventually leading to tooth loss, is one of the two biggest threats to oral health. Beyond oral health, it is associated with systemic diseases and even with cancer risk. Obviously, periodontitis represents a...
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doaj-a2236d3d22444929ba54afc34f3d70892020-12-30T00:05:05ZengMDPI AGMicroorganisms2076-26072021-12-019727210.3390/microorganisms9010072Detection of Epstein–Barr Virus in Periodontitis: A Review of Methodological ApproachesLilit Tonoyan0Marlène Chevalier1Séverine Vincent-Bugnas2Robert Marsault3Alain Doglio4MICORALIS, Faculté de Chirurgie Dentaire, Université Côte D’Azur, 5 rue du 22ième BCA, 06357 Nice, FranceMICORALIS, Faculté de Chirurgie Dentaire, Université Côte D’Azur, 5 rue du 22ième BCA, 06357 Nice, FranceMICORALIS, Faculté de Chirurgie Dentaire, Université Côte D’Azur, 5 rue du 22ième BCA, 06357 Nice, FranceMICORALIS, Faculté de Chirurgie Dentaire, Université Côte D’Azur, 5 rue du 22ième BCA, 06357 Nice, FranceMICORALIS, Faculté de Chirurgie Dentaire, Université Côte D’Azur, 5 rue du 22ième BCA, 06357 Nice, FrancePeriodontitis, an inflammatory condition that affects the structures surrounding the tooth eventually leading to tooth loss, is one of the two biggest threats to oral health. Beyond oral health, it is associated with systemic diseases and even with cancer risk. Obviously, periodontitis represents a major global health problem with significant social and economic impact. Recently, a new paradigm was proposed in the etiopathogenesis of periodontitis involving a herpesviral–bacterial combination to promote long-term chronic inflammatory disease. Periodontitis as a risk factor for other systemic diseases can also be better explained based on viral–bacterial etiology. Significant efforts have brought numerous advances in revealing the links between periodontitis and Epstein–Barr virus (EBV), a gamma herpesvirus ubiquitous in the adult human population. The strong evidence from these studies may contribute to the advancement of periodontitis research and the ultimate control of the disease. Advancing the periodontitis research will require implementing suitable methods to establish EBV involvement in periodontitis. This review evaluates and summarizes the existing methods that allow the detection and diagnosis of EBV in periodontitis (also applicable in a more general way to other EBV-related diseases), and discusses the feasibility of the application of innovative emerging technologies.https://www.mdpi.com/2076-2607/9/1/72Epstein–Barr virusperiodontitisdetection methodsEBER-ISHPCR-based methodsimmunohistochemistry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lilit Tonoyan Marlène Chevalier Séverine Vincent-Bugnas Robert Marsault Alain Doglio |
spellingShingle |
Lilit Tonoyan Marlène Chevalier Séverine Vincent-Bugnas Robert Marsault Alain Doglio Detection of Epstein–Barr Virus in Periodontitis: A Review of Methodological Approaches Microorganisms Epstein–Barr virus periodontitis detection methods EBER-ISH PCR-based methods immunohistochemistry |
author_facet |
Lilit Tonoyan Marlène Chevalier Séverine Vincent-Bugnas Robert Marsault Alain Doglio |
author_sort |
Lilit Tonoyan |
title |
Detection of Epstein–Barr Virus in Periodontitis: A Review of Methodological Approaches |
title_short |
Detection of Epstein–Barr Virus in Periodontitis: A Review of Methodological Approaches |
title_full |
Detection of Epstein–Barr Virus in Periodontitis: A Review of Methodological Approaches |
title_fullStr |
Detection of Epstein–Barr Virus in Periodontitis: A Review of Methodological Approaches |
title_full_unstemmed |
Detection of Epstein–Barr Virus in Periodontitis: A Review of Methodological Approaches |
title_sort |
detection of epstein–barr virus in periodontitis: a review of methodological approaches |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2021-12-01 |
description |
Periodontitis, an inflammatory condition that affects the structures surrounding the tooth eventually leading to tooth loss, is one of the two biggest threats to oral health. Beyond oral health, it is associated with systemic diseases and even with cancer risk. Obviously, periodontitis represents a major global health problem with significant social and economic impact. Recently, a new paradigm was proposed in the etiopathogenesis of periodontitis involving a herpesviral–bacterial combination to promote long-term chronic inflammatory disease. Periodontitis as a risk factor for other systemic diseases can also be better explained based on viral–bacterial etiology. Significant efforts have brought numerous advances in revealing the links between periodontitis and Epstein–Barr virus (EBV), a gamma herpesvirus ubiquitous in the adult human population. The strong evidence from these studies may contribute to the advancement of periodontitis research and the ultimate control of the disease. Advancing the periodontitis research will require implementing suitable methods to establish EBV involvement in periodontitis. This review evaluates and summarizes the existing methods that allow the detection and diagnosis of EBV in periodontitis (also applicable in a more general way to other EBV-related diseases), and discusses the feasibility of the application of innovative emerging technologies. |
topic |
Epstein–Barr virus periodontitis detection methods EBER-ISH PCR-based methods immunohistochemistry |
url |
https://www.mdpi.com/2076-2607/9/1/72 |
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