Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies
Abstract Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-base...
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doaj-a3c93244a0554695a83601c593ff08db2020-11-25T01:41:10ZengBMCVirology Journal1743-422X2018-12-011511610.1186/s12985-018-1105-5Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studiesElif Nurtop0Paola Mariela Saba Villarroel1Boris Pastorino2Laetitia Ninove3Jan Felix Drexler4Yelin Roca5Bouba Gake6Audrey Dubot-Peres7Gilda Grard8Christophe Peyrefitte9Stéphane Priet10Xavier de Lamballerie11Pierre Gallian12Unité des Virus Émergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection)Unité des Virus Émergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection)Unité des Virus Émergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection)Unité des Virus Émergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection)Institute of Virology, Charité-Universitätsmedizin BerlinVirología II, Centro Nacional de Enfermedades Tropicales (CENETROP)Unité des Virus Émergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection)Unité des Virus Émergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection)Unité des Virus Émergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection)Unité des Virus Émergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection)Unité des Virus Émergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection)Unité des Virus Émergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection)Unité des Virus Émergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection)Abstract Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-based VNT). In post-epidemic samples from Martinique Island blood donors (a population with a dengue seroprevalence above 90%), this strategy allowed reaching specificity and sensitivity values over 98%. The CPE-based VNT consists of recording CPE directly under the optical microscope, which is easy to identify with ZIKV strain H/PF/2013 at day 5 pi. Overall, considered that CPE-based VNT is cost effective and widely automatable, the NS1 protein-based Zika IgG ELISA+CPE-based VNT combination strategy represents a convenient tool to expedite ZIKV seroprevalence studies.http://link.springer.com/article/10.1186/s12985-018-1105-5Zika virusSeroepidemiologyVirus neutralization test |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elif Nurtop Paola Mariela Saba Villarroel Boris Pastorino Laetitia Ninove Jan Felix Drexler Yelin Roca Bouba Gake Audrey Dubot-Peres Gilda Grard Christophe Peyrefitte Stéphane Priet Xavier de Lamballerie Pierre Gallian |
spellingShingle |
Elif Nurtop Paola Mariela Saba Villarroel Boris Pastorino Laetitia Ninove Jan Felix Drexler Yelin Roca Bouba Gake Audrey Dubot-Peres Gilda Grard Christophe Peyrefitte Stéphane Priet Xavier de Lamballerie Pierre Gallian Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies Virology Journal Zika virus Seroepidemiology Virus neutralization test |
author_facet |
Elif Nurtop Paola Mariela Saba Villarroel Boris Pastorino Laetitia Ninove Jan Felix Drexler Yelin Roca Bouba Gake Audrey Dubot-Peres Gilda Grard Christophe Peyrefitte Stéphane Priet Xavier de Lamballerie Pierre Gallian |
author_sort |
Elif Nurtop |
title |
Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies |
title_short |
Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies |
title_full |
Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies |
title_fullStr |
Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies |
title_full_unstemmed |
Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies |
title_sort |
combination of elisa screening and seroneutralisation tests to expedite zika virus seroprevalence studies |
publisher |
BMC |
series |
Virology Journal |
issn |
1743-422X |
publishDate |
2018-12-01 |
description |
Abstract Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-based VNT). In post-epidemic samples from Martinique Island blood donors (a population with a dengue seroprevalence above 90%), this strategy allowed reaching specificity and sensitivity values over 98%. The CPE-based VNT consists of recording CPE directly under the optical microscope, which is easy to identify with ZIKV strain H/PF/2013 at day 5 pi. Overall, considered that CPE-based VNT is cost effective and widely automatable, the NS1 protein-based Zika IgG ELISA+CPE-based VNT combination strategy represents a convenient tool to expedite ZIKV seroprevalence studies. |
topic |
Zika virus Seroepidemiology Virus neutralization test |
url |
http://link.springer.com/article/10.1186/s12985-018-1105-5 |
work_keys_str_mv |
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