Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies

Abstract Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-base...

Full description

Bibliographic Details
Main Authors: Elif Nurtop, Paola Mariela Saba Villarroel, Boris Pastorino, Laetitia Ninove, Jan Felix Drexler, Yelin Roca, Bouba Gake, Audrey Dubot-Peres, Gilda Grard, Christophe Peyrefitte, Stéphane Priet, Xavier de Lamballerie, Pierre Gallian
Format: Article
Language:English
Published: BMC 2018-12-01
Series:Virology Journal
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12985-018-1105-5
id doaj-a3c93244a0554695a83601c593ff08db
record_format Article
spelling doaj-a3c93244a0554695a83601c593ff08db2020-11-25T01:41:10ZengBMCVirology Journal1743-422X2018-12-011511610.1186/s12985-018-1105-5Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studiesElif Nurtop0Paola Mariela Saba Villarroel1Boris Pastorino2Laetitia Ninove3Jan Felix Drexler4Yelin Roca5Bouba Gake6Audrey Dubot-Peres7Gilda Grard8Christophe Peyrefitte9Stéphane Priet10Xavier de Lamballerie11Pierre Gallian12Unité des Virus Émergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection)Unité des Virus Émergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection)Unité des Virus Émergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection)Unité des Virus Émergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection)Institute of Virology, Charité-Universitätsmedizin BerlinVirología II, Centro Nacional de Enfermedades Tropicales (CENETROP)Unité des Virus Émergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection)Unité des Virus Émergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection)Unité des Virus Émergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection)Unité des Virus Émergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection)Unité des Virus Émergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection)Unité des Virus Émergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection)Unité des Virus Émergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection)Abstract Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-based VNT). In post-epidemic samples from Martinique Island blood donors (a population with a dengue seroprevalence above 90%), this strategy allowed reaching specificity and sensitivity values over 98%. The CPE-based VNT consists of recording CPE directly under the optical microscope, which is easy to identify with ZIKV strain H/PF/2013 at day 5 pi. Overall, considered that CPE-based VNT is cost effective and widely automatable, the NS1 protein-based Zika IgG ELISA+CPE-based VNT combination strategy represents a convenient tool to expedite ZIKV seroprevalence studies.http://link.springer.com/article/10.1186/s12985-018-1105-5Zika virusSeroepidemiologyVirus neutralization test
collection DOAJ
language English
format Article
sources DOAJ
author Elif Nurtop
Paola Mariela Saba Villarroel
Boris Pastorino
Laetitia Ninove
Jan Felix Drexler
Yelin Roca
Bouba Gake
Audrey Dubot-Peres
Gilda Grard
Christophe Peyrefitte
Stéphane Priet
Xavier de Lamballerie
Pierre Gallian
spellingShingle Elif Nurtop
Paola Mariela Saba Villarroel
Boris Pastorino
Laetitia Ninove
Jan Felix Drexler
Yelin Roca
Bouba Gake
Audrey Dubot-Peres
Gilda Grard
Christophe Peyrefitte
Stéphane Priet
Xavier de Lamballerie
Pierre Gallian
Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies
Virology Journal
Zika virus
Seroepidemiology
Virus neutralization test
author_facet Elif Nurtop
Paola Mariela Saba Villarroel
Boris Pastorino
Laetitia Ninove
Jan Felix Drexler
Yelin Roca
Bouba Gake
Audrey Dubot-Peres
Gilda Grard
Christophe Peyrefitte
Stéphane Priet
Xavier de Lamballerie
Pierre Gallian
author_sort Elif Nurtop
title Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies
title_short Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies
title_full Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies
title_fullStr Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies
title_full_unstemmed Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies
title_sort combination of elisa screening and seroneutralisation tests to expedite zika virus seroprevalence studies
publisher BMC
series Virology Journal
issn 1743-422X
publishDate 2018-12-01
description Abstract Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-based VNT). In post-epidemic samples from Martinique Island blood donors (a population with a dengue seroprevalence above 90%), this strategy allowed reaching specificity and sensitivity values over 98%. The CPE-based VNT consists of recording CPE directly under the optical microscope, which is easy to identify with ZIKV strain H/PF/2013 at day 5 pi. Overall, considered that CPE-based VNT is cost effective and widely automatable, the NS1 protein-based Zika IgG ELISA+CPE-based VNT combination strategy represents a convenient tool to expedite ZIKV seroprevalence studies.
topic Zika virus
Seroepidemiology
Virus neutralization test
url http://link.springer.com/article/10.1186/s12985-018-1105-5
work_keys_str_mv AT elifnurtop combinationofelisascreeningandseroneutralisationteststoexpeditezikavirusseroprevalencestudies
AT paolamarielasabavillarroel combinationofelisascreeningandseroneutralisationteststoexpeditezikavirusseroprevalencestudies
AT borispastorino combinationofelisascreeningandseroneutralisationteststoexpeditezikavirusseroprevalencestudies
AT laetitianinove combinationofelisascreeningandseroneutralisationteststoexpeditezikavirusseroprevalencestudies
AT janfelixdrexler combinationofelisascreeningandseroneutralisationteststoexpeditezikavirusseroprevalencestudies
AT yelinroca combinationofelisascreeningandseroneutralisationteststoexpeditezikavirusseroprevalencestudies
AT boubagake combinationofelisascreeningandseroneutralisationteststoexpeditezikavirusseroprevalencestudies
AT audreydubotperes combinationofelisascreeningandseroneutralisationteststoexpeditezikavirusseroprevalencestudies
AT gildagrard combinationofelisascreeningandseroneutralisationteststoexpeditezikavirusseroprevalencestudies
AT christophepeyrefitte combinationofelisascreeningandseroneutralisationteststoexpeditezikavirusseroprevalencestudies
AT stephanepriet combinationofelisascreeningandseroneutralisationteststoexpeditezikavirusseroprevalencestudies
AT xavierdelamballerie combinationofelisascreeningandseroneutralisationteststoexpeditezikavirusseroprevalencestudies
AT pierregallian combinationofelisascreeningandseroneutralisationteststoexpeditezikavirusseroprevalencestudies
_version_ 1725042222530822144