Local Controlled Release of Polyphenol Conjugated with Gelatin Facilitates Bone Formation
Catechins are extensively used in health care treatments. Nevertheless, there is scarce information about the feasibility of local administration with polyphenols for bone regeneration therapy, possibly due to lack of effective delivery systems. Here we demonstrated that the epigallocatechin-3-galla...
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doaj-a43da0f30ce04c6da1725cd871bb0fe42020-11-25T00:50:09ZengMDPI AGInternational Journal of Molecular Sciences1422-00672015-06-01166141431415710.3390/ijms160614143ijms160614143Local Controlled Release of Polyphenol Conjugated with Gelatin Facilitates Bone FormationYoshitomo Honda0Tomonari Tanaka1Tomoko Tokuda2Takahiro Kashiwagi3Koji Kaida4Ayato Hieda5Yasuyuki Umezaki6Yoshiya Hashimoto7Koichi Imai8Naoyuki Matsumoto9Shunsuke Baba10Kimishige Shimizutani11Institute of Dental Research, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata, Osaka 573-1121, JapanGraduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, JapanDepartment of Orthodontics, Osaka Dental University, 8-1, Kuzuhahanazonocho, Hirakata, Osaka 573-1121, JapanDepartment of Oral Implantology, Osaka Dental University; 8-1 Kuzuhahanazonocho, Hirakata, Osaka, 573-1121, JapanDepartment of Oral Implantology, Osaka Dental University; 8-1 Kuzuhahanazonocho, Hirakata, Osaka, 573-1121, JapanDepartment of Oral Implantology, Osaka Dental University; 8-1 Kuzuhahanazonocho, Hirakata, Osaka, 573-1121, JapanDepartment of Oral Implantology, Osaka Dental University; 8-1 Kuzuhahanazonocho, Hirakata, Osaka, 573-1121, JapanOsaka Dental University; 8-1 Kuzuhahanazonocho, Hirakata, Osaka 573-1121, JapanOsaka Dental University; 8-1 Kuzuhahanazonocho, Hirakata, Osaka 573-1121, JapanDepartment of Orthodontics, Osaka Dental University, 8-1, Kuzuhahanazonocho, Hirakata, Osaka 573-1121, JapanDepartment of Oral Implantology, Osaka Dental University; 8-1 Kuzuhahanazonocho, Hirakata, Osaka, 573-1121, JapanInstitute of Dental Research, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata, Osaka 573-1121, JapanCatechins are extensively used in health care treatments. Nevertheless, there is scarce information about the feasibility of local administration with polyphenols for bone regeneration therapy, possibly due to lack of effective delivery systems. Here we demonstrated that the epigallocatechin-3-gallate-conjugated gelatin (EGCG/Gel) prepared by an aqueous chemical synthesis using 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-morpholinium chloride (DMT-MM) gradually disintegrated with time and facilitated bone formation in a critical size defect of a mouse calvaria. Conjugation of EGCG with the Gel generated cross-linking between the two molecules, thereby leading to a retardation of the degradation of the EGCG/Gel and to a delayed release of EGCG. The prepared EGCG/Gels represented significant osteogenic capability compared with that of the uncross-linked Gel and the cross-linked Gel with uncombined-EGCG. In vitro experiments disclosed that the EGCG/Gel induced osteoblastogenesis of a mouse mesenchymal stem cell line (D1 cells) within 14 days. Using fluorescently-labeled EGCG/Gel, we found that the fraction of EGCG/Gel adsorbed onto the cell membrane of the D1 cells possibly via a Gel-cell interaction. The interaction might confer the long-term effects of EGCG on the cells, resulting in a potent osteogenic capability of the EGCG/Gel in vivo. These results should provide insight into local controlled release of polyphenols for bone therapy.http://www.mdpi.com/1422-0067/16/6/14143catechinEGCGbone formationgelatinmesenchymal stem cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yoshitomo Honda Tomonari Tanaka Tomoko Tokuda Takahiro Kashiwagi Koji Kaida Ayato Hieda Yasuyuki Umezaki Yoshiya Hashimoto Koichi Imai Naoyuki Matsumoto Shunsuke Baba Kimishige Shimizutani |
spellingShingle |
Yoshitomo Honda Tomonari Tanaka Tomoko Tokuda Takahiro Kashiwagi Koji Kaida Ayato Hieda Yasuyuki Umezaki Yoshiya Hashimoto Koichi Imai Naoyuki Matsumoto Shunsuke Baba Kimishige Shimizutani Local Controlled Release of Polyphenol Conjugated with Gelatin Facilitates Bone Formation International Journal of Molecular Sciences catechin EGCG bone formation gelatin mesenchymal stem cells |
author_facet |
Yoshitomo Honda Tomonari Tanaka Tomoko Tokuda Takahiro Kashiwagi Koji Kaida Ayato Hieda Yasuyuki Umezaki Yoshiya Hashimoto Koichi Imai Naoyuki Matsumoto Shunsuke Baba Kimishige Shimizutani |
author_sort |
Yoshitomo Honda |
title |
Local Controlled Release of Polyphenol Conjugated with Gelatin Facilitates Bone Formation |
title_short |
Local Controlled Release of Polyphenol Conjugated with Gelatin Facilitates Bone Formation |
title_full |
Local Controlled Release of Polyphenol Conjugated with Gelatin Facilitates Bone Formation |
title_fullStr |
Local Controlled Release of Polyphenol Conjugated with Gelatin Facilitates Bone Formation |
title_full_unstemmed |
Local Controlled Release of Polyphenol Conjugated with Gelatin Facilitates Bone Formation |
title_sort |
local controlled release of polyphenol conjugated with gelatin facilitates bone formation |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2015-06-01 |
description |
Catechins are extensively used in health care treatments. Nevertheless, there is scarce information about the feasibility of local administration with polyphenols for bone regeneration therapy, possibly due to lack of effective delivery systems. Here we demonstrated that the epigallocatechin-3-gallate-conjugated gelatin (EGCG/Gel) prepared by an aqueous chemical synthesis using 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-morpholinium chloride (DMT-MM) gradually disintegrated with time and facilitated bone formation in a critical size defect of a mouse calvaria. Conjugation of EGCG with the Gel generated cross-linking between the two molecules, thereby leading to a retardation of the degradation of the EGCG/Gel and to a delayed release of EGCG. The prepared EGCG/Gels represented significant osteogenic capability compared with that of the uncross-linked Gel and the cross-linked Gel with uncombined-EGCG. In vitro experiments disclosed that the EGCG/Gel induced osteoblastogenesis of a mouse mesenchymal stem cell line (D1 cells) within 14 days. Using fluorescently-labeled EGCG/Gel, we found that the fraction of EGCG/Gel adsorbed onto the cell membrane of the D1 cells possibly via a Gel-cell interaction. The interaction might confer the long-term effects of EGCG on the cells, resulting in a potent osteogenic capability of the EGCG/Gel in vivo. These results should provide insight into local controlled release of polyphenols for bone therapy. |
topic |
catechin EGCG bone formation gelatin mesenchymal stem cells |
url |
http://www.mdpi.com/1422-0067/16/6/14143 |
work_keys_str_mv |
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