Efficient Production of Scleroglucan by Sclerotium rolfsii and Insights Into Molecular Weight Modification by High-Pressure Homogenization
Scleroglucan is a non-ionic water-soluble polysaccharide, and has been widely used in the petroleum, food, medicine and cosmetics industries. Currently, scleroglucan is mainly produced by Sclerotium rolfsii. A higher level of scleroglucan (42.0 g/L) was previously obtained with S. rolfsii WSH-G01. H...
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Frontiers Media S.A.
2021-09-01
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doaj-a589cde24219403592a2b3d8f96df2a52021-09-04T09:29:10ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852021-09-01910.3389/fbioe.2021.748213748213Efficient Production of Scleroglucan by Sclerotium rolfsii and Insights Into Molecular Weight Modification by High-Pressure HomogenizationWeizhu Zeng0Weizhu Zeng1Weizhu Zeng2Junyi Wang3Junyi Wang4Junyi Wang5Junyi Wang6Xiaoyu Shan7Xiaoyu Shan8Xiaoyu Shan9Xiaoyu Shan10Shiqin Yu11Shiqin Yu12Shiqin Yu13Jingwen Zhou14Jingwen Zhou15Jingwen Zhou16Jingwen Zhou17National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, ChinaScience Center for Future Foods, Jiangnan University, Wuxi, ChinaKey Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, ChinaNational Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, ChinaScience Center for Future Foods, Jiangnan University, Wuxi, ChinaKey Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, ChinaJiangsu Provisional Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi, ChinaNational Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, ChinaScience Center for Future Foods, Jiangnan University, Wuxi, ChinaKey Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, ChinaJiangsu Provisional Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi, ChinaNational Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, ChinaKey Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, ChinaJiangsu Provisional Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi, ChinaNational Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, ChinaScience Center for Future Foods, Jiangnan University, Wuxi, ChinaKey Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, ChinaJiangsu Provisional Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi, ChinaScleroglucan is a non-ionic water-soluble polysaccharide, and has been widely used in the petroleum, food, medicine and cosmetics industries. Currently, scleroglucan is mainly produced by Sclerotium rolfsii. A higher level of scleroglucan (42.0 g/L) was previously obtained with S. rolfsii WSH-G01. However, the production of scleroglucan was reduced despite a higher glucose concentration remaining. Additionally, the molecular weight of scleroglucan was large, thus restricted its application. In this study, by adjusting the state of seeds inoculated, the degradation issue of scleroglucan during the fermentation process was solved. By comparing different fed-batch strategies, 66.6 g/L of scleroglucan was harvested by a two-dose fed-batch mode, with 53.3% glucose conversion ratio. To modify the molecular weight of scleroglucan, a combination method with HCl and high-pressure homogenization treatment was established. Finally, scleroglucan with molecular weight of 4.61 × 105 Da was obtained. The developed approaches provide references for the biosynthesis and molecular weight modification of polysaccharides.https://www.frontiersin.org/articles/10.3389/fbioe.2021.748213/fullSclerotium rolfsiiscleroglucanfed-batchmolecular weighthigh-pressure homogenization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Weizhu Zeng Weizhu Zeng Weizhu Zeng Junyi Wang Junyi Wang Junyi Wang Junyi Wang Xiaoyu Shan Xiaoyu Shan Xiaoyu Shan Xiaoyu Shan Shiqin Yu Shiqin Yu Shiqin Yu Jingwen Zhou Jingwen Zhou Jingwen Zhou Jingwen Zhou |
spellingShingle |
Weizhu Zeng Weizhu Zeng Weizhu Zeng Junyi Wang Junyi Wang Junyi Wang Junyi Wang Xiaoyu Shan Xiaoyu Shan Xiaoyu Shan Xiaoyu Shan Shiqin Yu Shiqin Yu Shiqin Yu Jingwen Zhou Jingwen Zhou Jingwen Zhou Jingwen Zhou Efficient Production of Scleroglucan by Sclerotium rolfsii and Insights Into Molecular Weight Modification by High-Pressure Homogenization Frontiers in Bioengineering and Biotechnology Sclerotium rolfsii scleroglucan fed-batch molecular weight high-pressure homogenization |
author_facet |
Weizhu Zeng Weizhu Zeng Weizhu Zeng Junyi Wang Junyi Wang Junyi Wang Junyi Wang Xiaoyu Shan Xiaoyu Shan Xiaoyu Shan Xiaoyu Shan Shiqin Yu Shiqin Yu Shiqin Yu Jingwen Zhou Jingwen Zhou Jingwen Zhou Jingwen Zhou |
author_sort |
Weizhu Zeng |
title |
Efficient Production of Scleroglucan by Sclerotium rolfsii and Insights Into Molecular Weight Modification by High-Pressure Homogenization |
title_short |
Efficient Production of Scleroglucan by Sclerotium rolfsii and Insights Into Molecular Weight Modification by High-Pressure Homogenization |
title_full |
Efficient Production of Scleroglucan by Sclerotium rolfsii and Insights Into Molecular Weight Modification by High-Pressure Homogenization |
title_fullStr |
Efficient Production of Scleroglucan by Sclerotium rolfsii and Insights Into Molecular Weight Modification by High-Pressure Homogenization |
title_full_unstemmed |
Efficient Production of Scleroglucan by Sclerotium rolfsii and Insights Into Molecular Weight Modification by High-Pressure Homogenization |
title_sort |
efficient production of scleroglucan by sclerotium rolfsii and insights into molecular weight modification by high-pressure homogenization |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Bioengineering and Biotechnology |
issn |
2296-4185 |
publishDate |
2021-09-01 |
description |
Scleroglucan is a non-ionic water-soluble polysaccharide, and has been widely used in the petroleum, food, medicine and cosmetics industries. Currently, scleroglucan is mainly produced by Sclerotium rolfsii. A higher level of scleroglucan (42.0 g/L) was previously obtained with S. rolfsii WSH-G01. However, the production of scleroglucan was reduced despite a higher glucose concentration remaining. Additionally, the molecular weight of scleroglucan was large, thus restricted its application. In this study, by adjusting the state of seeds inoculated, the degradation issue of scleroglucan during the fermentation process was solved. By comparing different fed-batch strategies, 66.6 g/L of scleroglucan was harvested by a two-dose fed-batch mode, with 53.3% glucose conversion ratio. To modify the molecular weight of scleroglucan, a combination method with HCl and high-pressure homogenization treatment was established. Finally, scleroglucan with molecular weight of 4.61 × 105 Da was obtained. The developed approaches provide references for the biosynthesis and molecular weight modification of polysaccharides. |
topic |
Sclerotium rolfsii scleroglucan fed-batch molecular weight high-pressure homogenization |
url |
https://www.frontiersin.org/articles/10.3389/fbioe.2021.748213/full |
work_keys_str_mv |
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