Transcriptomic and Functional Studies of the RGS Protein Rax1 in <i>Aspergillus fumigatus</i>

Transcriptomic and Functional Studies of the RGS Protein Rax1 in <i>Aspergillus fumigatus</i>

In the comparative transcriptomic studies of wild type (WT) and <i>rax1</i> null mutant strains, we obtained an average of 22,222,727 reads of 101 bp per sample and found that 183 genes showed greater than 2.0-fold differential expression, where 92 and 91 genes were up-and down-regulated...

Full description

Bibliographic Details
Main Authors: Yong-Ho Choi, Min-Woo Lee, Olumuyiwa Ayokunle Igbalajobi, Jae-Hyuk Yu, Kwang-Soo Shin
Format: Article
Language:English
Published: MDPI AG 2019-12-01
Series:Pathogens
Subjects:
<i>aspergillus fumigatus</i>
glim
metacaspase
rax1
rna-seq
Online Access:https://www.mdpi.com/2076-0817/9/1/36
id doaj-a5fc434d8ce74d4e94b687ef5f3b5102
record_format Article
spelling doaj-a5fc434d8ce74d4e94b687ef5f3b51022020-11-25T02:36:52ZengMDPI AGPathogens2076-08172019-12-01913610.3390/pathogens9010036pathogens9010036Transcriptomic and Functional Studies of the RGS Protein Rax1 in <i>Aspergillus fumigatus</i>Yong-Ho Choi0Min-Woo Lee1Olumuyiwa Ayokunle Igbalajobi2Jae-Hyuk Yu3Kwang-Soo Shin4Department of Microbiology, Graduate School, Daejeon University, Daejeon 34520, KoreaSoonchunhyang Institute of Medi-bio Science, Soonchunhyang University, Chungcheongnam-do 31151, KoreaDepartment of Microbiology, Institute for Applied Biosciences, Karlsruhe Institute of Technology, Fritz-Haber Weg 4, D-76131 Karlsruhe, GermanyDepartments of Bacteriology and Genetics, University of Wisconsin-Madison, Madison, WI 53706, USADepartment of Microbiology, Graduate School, Daejeon University, Daejeon 34520, KoreaIn the comparative transcriptomic studies of wild type (WT) and <i>rax1</i> null mutant strains, we obtained an average of 22,222,727 reads of 101 bp per sample and found that 183 genes showed greater than 2.0-fold differential expression, where 92 and 91 genes were up-and down-regulated in &#916;<i>rax1</i> compared to WT, respectively. In accordance with the significantly reduced levels of <i>gliM</i> and <i>casB</i> transcripts in the absence of <i>rax1</i>, the &#916;<i>rax1</i> mutant exhibited increased sensitivity to exogenous gliotoxin (GT) without affecting levels of GT production. Moreover, &#916;<i>rax1</i> resulted in significantly restricted colony growth and reduced viability under endoplasmic reticulum stress condition. In summary, Rax1 positively affects expression of <i>gliM</i> and metacaspase genes.https://www.mdpi.com/2076-0817/9/1/36<i>aspergillus fumigatus</i>glimmetacaspaserax1rna-seq
collection DOAJ
language English
format Article
sources DOAJ
author Yong-Ho Choi
Min-Woo Lee
Olumuyiwa Ayokunle Igbalajobi
Jae-Hyuk Yu
Kwang-Soo Shin
spellingShingle Yong-Ho Choi
Min-Woo Lee
Olumuyiwa Ayokunle Igbalajobi
Jae-Hyuk Yu
Kwang-Soo Shin
Transcriptomic and Functional Studies of the RGS Protein Rax1 in <i>Aspergillus fumigatus</i>
Pathogens
<i>aspergillus fumigatus</i>
glim
metacaspase
rax1
rna-seq
author_facet Yong-Ho Choi
Min-Woo Lee
Olumuyiwa Ayokunle Igbalajobi
Jae-Hyuk Yu
Kwang-Soo Shin
author_sort Yong-Ho Choi
title Transcriptomic and Functional Studies of the RGS Protein Rax1 in <i>Aspergillus fumigatus</i>
title_short Transcriptomic and Functional Studies of the RGS Protein Rax1 in <i>Aspergillus fumigatus</i>
title_full Transcriptomic and Functional Studies of the RGS Protein Rax1 in <i>Aspergillus fumigatus</i>
title_fullStr Transcriptomic and Functional Studies of the RGS Protein Rax1 in <i>Aspergillus fumigatus</i>
title_full_unstemmed Transcriptomic and Functional Studies of the RGS Protein Rax1 in <i>Aspergillus fumigatus</i>
title_sort transcriptomic and functional studies of the rgs protein rax1 in <i>aspergillus fumigatus</i>
publisher MDPI AG
series Pathogens
issn 2076-0817
publishDate 2019-12-01
description In the comparative transcriptomic studies of wild type (WT) and <i>rax1</i> null mutant strains, we obtained an average of 22,222,727 reads of 101 bp per sample and found that 183 genes showed greater than 2.0-fold differential expression, where 92 and 91 genes were up-and down-regulated in &#916;<i>rax1</i> compared to WT, respectively. In accordance with the significantly reduced levels of <i>gliM</i> and <i>casB</i> transcripts in the absence of <i>rax1</i>, the &#916;<i>rax1</i> mutant exhibited increased sensitivity to exogenous gliotoxin (GT) without affecting levels of GT production. Moreover, &#916;<i>rax1</i> resulted in significantly restricted colony growth and reduced viability under endoplasmic reticulum stress condition. In summary, Rax1 positively affects expression of <i>gliM</i> and metacaspase genes.
topic <i>aspergillus fumigatus</i>
glim
metacaspase
rax1
rna-seq
url https://www.mdpi.com/2076-0817/9/1/36
work_keys_str_mv AT yonghochoi transcriptomicandfunctionalstudiesofthergsproteinrax1iniaspergillusfumigatusi
AT minwoolee transcriptomicandfunctionalstudiesofthergsproteinrax1iniaspergillusfumigatusi
AT olumuyiwaayokunleigbalajobi transcriptomicandfunctionalstudiesofthergsproteinrax1iniaspergillusfumigatusi
AT jaehyukyu transcriptomicandfunctionalstudiesofthergsproteinrax1iniaspergillusfumigatusi
AT kwangsooshin transcriptomicandfunctionalstudiesofthergsproteinrax1iniaspergillusfumigatusi
_version_ 1724798288078569472

Similar Items