Systematic Methodological Evaluation of a Multiplex Bead-Based Flow Cytometry Assay for Detection of Extracellular Vesicle Surface Signatures

Systematic Methodological Evaluation of a Multiplex Bead-Based Flow Cytometry Assay for Detection of Extracellular Vesicle Surface Signatures

Extracellular vesicles (EVs) can be harvested from cell culture supernatants and from all body fluids. EVs can be conceptually classified based on their size and biogenesis as exosomes and microvesicles. Nowadays, it is however commonly accepted in the field that there is a much higher degree of het...

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Bibliographic Details
Main Authors: Oscar P. B. Wiklander, R. Beklem Bostancioglu, Joshua A. Welsh, Antje M. Zickler, Florian Murke, Giulia Corso, Ulrika Felldin, Daniel W. Hagey, Björn Evertsson, Xiu-Ming Liang, Manuela O. Gustafsson, Dara K. Mohammad, Constanze Wiek, Helmut Hanenberg, Michel Bremer, Dhanu Gupta, Mikael Björnstedt, Bernd Giebel, Joel Z. Nordin, Jennifer C. Jones, Samir EL Andaloussi, André Görgens
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-06-01
Series:Frontiers in Immunology
Subjects:
exosomes
microvesicles
extracellular vesicles
extracellular vesicle flow cytometry
bead-based flow cytometry
exosome analysis
Online Access:https://www.frontiersin.org/article/10.3389/fimmu.2018.01326/full

Internet

https://www.frontiersin.org/article/10.3389/fimmu.2018.01326/full

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