DCX and CRABP2 are candidate genes for differential diagnosis between pre‐chemotherapy embryonic and alveolar rhabdomyosarcoma in pediatric patients
ABSTRACT Importance Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma in children. More than 90% of cases are classified as embryonic RMS (ERMS) or alveolar RMS (ARMS). ERMS has a worse prognosis than ARMS. Early differential diagnosis is of paramount importance for optimization of treat...
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| Format: | Article |
| Language: | English |
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Wiley
2021-06-01
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| Series: | Pediatric Investigation |
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| Online Access: | https://doi.org/10.1002/ped4.12278 |
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doaj-a7de2114d4ec4a699d59cf476ba3608f |
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Article |
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DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Nian Sun Yeran Yang Shengcai Wang Jie Zhang Jingang Gui Jun Tai Lejian He Jiatong Xu Yanzhen Li Xuexi Zhang Qiaoyin Liu Zhiyong Liu Yongli Guo Xin Ni |
| spellingShingle |
Nian Sun Yeran Yang Shengcai Wang Jie Zhang Jingang Gui Jun Tai Lejian He Jiatong Xu Yanzhen Li Xuexi Zhang Qiaoyin Liu Zhiyong Liu Yongli Guo Xin Ni DCX and CRABP2 are candidate genes for differential diagnosis between pre‐chemotherapy embryonic and alveolar rhabdomyosarcoma in pediatric patients Pediatric Investigation Alveolar rhabdomyosarcoma Embryonic rhabdomyosarcoma DCX CRABP2 Immunohistochemistry |
| author_facet |
Nian Sun Yeran Yang Shengcai Wang Jie Zhang Jingang Gui Jun Tai Lejian He Jiatong Xu Yanzhen Li Xuexi Zhang Qiaoyin Liu Zhiyong Liu Yongli Guo Xin Ni |
| author_sort |
Nian Sun |
| title |
DCX and CRABP2 are candidate genes for differential diagnosis between pre‐chemotherapy embryonic and alveolar rhabdomyosarcoma in pediatric patients |
| title_short |
DCX and CRABP2 are candidate genes for differential diagnosis between pre‐chemotherapy embryonic and alveolar rhabdomyosarcoma in pediatric patients |
| title_full |
DCX and CRABP2 are candidate genes for differential diagnosis between pre‐chemotherapy embryonic and alveolar rhabdomyosarcoma in pediatric patients |
| title_fullStr |
DCX and CRABP2 are candidate genes for differential diagnosis between pre‐chemotherapy embryonic and alveolar rhabdomyosarcoma in pediatric patients |
| title_full_unstemmed |
DCX and CRABP2 are candidate genes for differential diagnosis between pre‐chemotherapy embryonic and alveolar rhabdomyosarcoma in pediatric patients |
| title_sort |
dcx and crabp2 are candidate genes for differential diagnosis between pre‐chemotherapy embryonic and alveolar rhabdomyosarcoma in pediatric patients |
| publisher |
Wiley |
| series |
Pediatric Investigation |
| issn |
2574-2272 |
| publishDate |
2021-06-01 |
| description |
ABSTRACT Importance Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma in children. More than 90% of cases are classified as embryonic RMS (ERMS) or alveolar RMS (ARMS). ERMS has a worse prognosis than ARMS. Early differential diagnosis is of paramount importance for optimization of treatment. Objective To identify genes that are differentially expressed between ARMS and ERMS, which can be used for accurate rhabdomyosarcoma classification. Methods Three Gene Expression Omnibus datasets composed of ARMS and ERMS samples were screened and 35 differentially expressed genes (DEGs) were identified. Receiver operating characteristic curve analysis and area under the curve analysis was performed for these 35 DEGs and seven candidate genes with the best differential expression scores between ARMS and ERMS were determined. The expression of these seven candidate genes was validated by immunohistochemical analysis of pre‐chemotherapy ARMS and ERMS specimens. Results The levels of DCX and CRABP2 were confirmed to be remarkably different between paraffin‐embedded ARMS and ERMS tissues, while EGFR abundance was only marginally different between these two RMS subtypes. Interpretation DCX and CRABP2 are potential biomarkers for distinguishing ARMS from ERMS in pre‐chemotherapy pediatric patients. |
| topic |
Alveolar rhabdomyosarcoma Embryonic rhabdomyosarcoma DCX CRABP2 Immunohistochemistry |
| url |
https://doi.org/10.1002/ped4.12278 |
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doaj-a7de2114d4ec4a699d59cf476ba3608f2021-06-18T13:10:38ZengWileyPediatric Investigation2574-22722021-06-015210611110.1002/ped4.12278DCX and CRABP2 are candidate genes for differential diagnosis between pre‐chemotherapy embryonic and alveolar rhabdomyosarcoma in pediatric patientsNian Sun0Yeran Yang1Shengcai Wang2Jie Zhang3Jingang Gui4Jun Tai5Lejian He6Jiatong Xu7Yanzhen Li8Xuexi Zhang9Qiaoyin Liu10Zhiyong Liu11Yongli Guo12Xin Ni13Department of Otolaryngology, Head and Neck Surgery Beijing Children’s Hospital Capital Medical University National Center for Children’s Health Beijing ChinaBeijing Key Laboratory for Pediatric Diseases of Otolaryngology Head and Neck Surgery MOE Key Laboratory of Major Diseases in Children Beijing Pediatric Research Institute Beijing Children’s Hospital Capital Medical University National Center for Children’s Health Beijing ChinaDepartment of Otolaryngology, Head and Neck Surgery Beijing Children’s Hospital Capital Medical University National Center for Children’s Health Beijing ChinaDepartment of Otolaryngology, Head and Neck Surgery Beijing Children’s Hospital Capital Medical University National Center for Children’s Health Beijing ChinaLaboratory of Tumor Immunology Beijing Pediatric Research Institute Beijing Children’s Hospital Capital Medical University National Center for Children’s Health Beijing ChinaDepartment of Otorhinolaryngology Children’s Hospital Capital Institute of Pediatrics Beijing ChinaDepartment of Pathology Beijing Children’s Hospital Capital Medical University National Center for Children’s Health Beijing ChinaDepartment of Pathology Beijing Children’s Hospital Capital Medical University National Center for Children’s Health Beijing ChinaDepartment of Otolaryngology, Head and Neck Surgery Beijing Children’s Hospital Capital Medical University National Center for Children’s Health Beijing ChinaDepartment of Otolaryngology, Head and Neck Surgery Beijing Children’s Hospital Capital Medical University National Center for Children’s Health Beijing ChinaDepartment of Otolaryngology, Head and Neck Surgery Beijing Children’s Hospital Capital Medical University National Center for Children’s Health Beijing ChinaDepartment of Otolaryngology, Head and Neck Surgery Beijing Children’s Hospital Capital Medical University National Center for Children’s Health Beijing ChinaBeijing Key Laboratory for Pediatric Diseases of Otolaryngology Head and Neck Surgery MOE Key Laboratory of Major Diseases in Children Beijing Pediatric Research Institute Beijing Children’s Hospital Capital Medical University National Center for Children’s Health Beijing ChinaDepartment of Otolaryngology, Head and Neck Surgery Beijing Children’s Hospital Capital Medical University National Center for Children’s Health Beijing ChinaABSTRACT Importance Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma in children. More than 90% of cases are classified as embryonic RMS (ERMS) or alveolar RMS (ARMS). ERMS has a worse prognosis than ARMS. Early differential diagnosis is of paramount importance for optimization of treatment. Objective To identify genes that are differentially expressed between ARMS and ERMS, which can be used for accurate rhabdomyosarcoma classification. Methods Three Gene Expression Omnibus datasets composed of ARMS and ERMS samples were screened and 35 differentially expressed genes (DEGs) were identified. Receiver operating characteristic curve analysis and area under the curve analysis was performed for these 35 DEGs and seven candidate genes with the best differential expression scores between ARMS and ERMS were determined. The expression of these seven candidate genes was validated by immunohistochemical analysis of pre‐chemotherapy ARMS and ERMS specimens. Results The levels of DCX and CRABP2 were confirmed to be remarkably different between paraffin‐embedded ARMS and ERMS tissues, while EGFR abundance was only marginally different between these two RMS subtypes. Interpretation DCX and CRABP2 are potential biomarkers for distinguishing ARMS from ERMS in pre‐chemotherapy pediatric patients.https://doi.org/10.1002/ped4.12278Alveolar rhabdomyosarcomaEmbryonic rhabdomyosarcomaDCXCRABP2Immunohistochemistry |