Identification of Two Novel Wheat Drought Tolerance-Related Proteins by Comparative Proteomic Analysis Combined with Virus-Induced Gene Silencing

• Main Authors: Xinbo Wang, Yanhua Xu, Jingjing Li, Yongzhe Ren, Zhiqiang Wang, Zeyu Xin, Tongbao Lin
• Format: Article
• Language: English
• Published: MDPI AG 2018-12-01
• Series: International Journal of Molecular Sciences
• Subjects: drought stress; <i>Triticum aestivum</i> L.; comparative proteomic analysis; iTRAQ; VIGS
• Online Access: https://www.mdpi.com/1422-0067/19/12/4020

Drought is a major adversity that limits crop yields. Further exploration of wheat drought tolerance-related genes is critical for the genetic improvement of drought tolerance in this crop. Here, comparative proteomic analysis of two wheat varieties, XN979 and LA379, with contrasting drought tolerance was conducted to screen for drought tolerance-related proteins/genes. Virus-induced gene silencing (VIGS) technology was used to verify the functions of candidate proteins. A total of 335 differentially abundant proteins (DAPs) were exclusively identified in the drought-tolerant variety XN979. Most DAPs were mainly involved in photosynthesis, carbon fixation, glyoxylate and dicarboxylate metabolism, and several other pathways. Two DAPs (W5DYH0 and W5ERN8), dubbed <i>TaDrSR1</i> and <i>TaDrSR2</i>, respectively, were selected for further functional analysis using VIGS. The relative electrolyte leakage rate and malonaldehyde content increased significantly, while the relative water content and proline content significantly decreased in the <i>TaDrSR1-</i> and <i>TaDrSR2</i>-knock-down plants compared to that in non-knocked-down plants under drought stress conditions. <i>TaDrSR1-</i> and <i>TaDrSR2</i>-knock-down plants exhibited more severe drooping and wilting phenotypes than non-knocked-down plants under drought stress conditions, suggesting that the former were more sensitive to drought stress. These results indicate that <i>TaDrSR1</i> and <i>TaDrSR2</i> potentially play vital roles in conferring drought tolerance in common wheat.