Ιn vivo imaging of DNA-bound minichromosome maintenance complex in embryonic mouse cortex

Ιn vivo imaging of DNA-bound minichromosome maintenance complex in embryonic mouse cortex

Summary: The recruitment of the minichromosome maintenance complex (MCM) on DNA replication origins is a critical process for faithful genome duplication termed licensing. Aberrant licensing has been associated with cancer and, recently, with neurodevelopmental diseases. Investigating MCM loading in...

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Bibliographic Details
Main Authors: Maria Mougkogianni, Argyro Kalogeropoulou, Nickolaos Nikiforos Giakoumakis, Zoi Lygerou, Stavros Taraviras
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Cell Biology
Microscopy
Model Organisms
Molecular Biology
Neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720302215
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Summary:Summary: The recruitment of the minichromosome maintenance complex (MCM) on DNA replication origins is a critical process for faithful genome duplication termed licensing. Aberrant licensing has been associated with cancer and, recently, with neurodevelopmental diseases. Investigating MCM loading in complicated tissues, such as brain, remains challenging. Here, we describe an optimized approach for the qualitative and quantitative analysis of DNA-bound MCMs in the developing mouse cortex through direct imaging, offering an innovative insight into the research of origin licensing in vivo.
ISSN:2666-1667

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