The signs of differentiation of chondrocytes in the formation of early cartilage lesions in the elderly

Objective. To study the relationship between the activity of collagen type II cleavage and the pattern of expression of the genes responsible for chondrocyte differentiation in the areas of intact cartilaginous tissue and in those of early focal cartilage lesions similar to those observed in elderly...

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Main Authors: Elena Vasil'evna Chetina, Elena Vasilyevna Chetina
Format: Article
Language:Russian
Published: IMA-PRESS LLC 2011-02-01
Series:Научно-практическая ревматология
Subjects:
Online Access:https://rsp.mediar-press.net/rsp/article/view/998
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spelling doaj-a895b4c81a764df08bf7a2d39f201a552021-08-02T09:05:42ZrusIMA-PRESS LLCНаучно-практическая ревматология1995-44841995-44922011-02-01491333910.14412/1995-4484-2011-865938The signs of differentiation of chondrocytes in the formation of early cartilage lesions in the elderlyElena Vasil'evna ChetinaElena Vasilyevna ChetinaObjective. To study the relationship between the activity of collagen type II cleavage and the pattern of expression of the genes responsible for chondrocyte differentiation in the areas of intact cartilaginous tissue and in those of early focal cartilage lesions similar to those observed in elderly people with osteoarthrosis (OA). Material and methods. The distal femoral articular surface of the knee joint that articulated with the patella and had focal lesions was examined in the elderly. Collagen type II cleavage was estimated by enzyme immunoassay. Gene expression was determined with reverse-transcription polymerase chain reaction. Results. The activity of collagen type II cleavage was shown to be increased in the area of age-related OA-like cartilage lesions. This was accompanied by the high expression of collagenases of metalloproteinases (MMP) 1, 14 (MT1-MMP), aggrecanases - desintegrin and MMP with thrombospondin type 1 motif (ADAMTS) 5, the cytokines of interleukins (IL) 1α/β and tumor necrosis factor-α (TNF-α), as well as the genes associated with chondrocyte hypertrophy of type X collagen (C0L10A1), MMP 13 and 9, Indian hedgehog (Ihh) and cas-pase 3 in the immediate vicinity of a lesion area. At the same time, there was a high expression of growth factors associated with the proliferation phase of chondrocytes, namely: parathyroid hormone-related peptide (PTHrP), fibroblast growth factor-2 (FGF-2), transforming growth factor β1/2 (TGF-β1/2), as well as macromolecules of matrix of type II collagen (C0L2A1) and aggrecan in both the areas adjacent to the lesions and at a considerable distance from their center. However, these areas showed no higher collagen cleavage activity. Nether higher collagen cleavage, nor excess expression of the genes examined were observed in the absolutely intact cartilage areas. Conclusion. Our studies have indicated that the area of very early age-related OA-like focal cartilage lesions exhibits enhanced type II collagen cleavage that is attended by the expression of the genes associated with chondrocyte differentiation in the embryonic growth plate.https://rsp.mediar-press.net/rsp/article/view/998articular cartilagechondrocyte differentiationgene expressionfocal lesions
collection DOAJ
language Russian
format Article
sources DOAJ
author Elena Vasil'evna Chetina
Elena Vasilyevna Chetina
spellingShingle Elena Vasil'evna Chetina
Elena Vasilyevna Chetina
The signs of differentiation of chondrocytes in the formation of early cartilage lesions in the elderly
Научно-практическая ревматология
articular cartilage
chondrocyte differentiation
gene expression
focal lesions
author_facet Elena Vasil'evna Chetina
Elena Vasilyevna Chetina
author_sort Elena Vasil'evna Chetina
title The signs of differentiation of chondrocytes in the formation of early cartilage lesions in the elderly
title_short The signs of differentiation of chondrocytes in the formation of early cartilage lesions in the elderly
title_full The signs of differentiation of chondrocytes in the formation of early cartilage lesions in the elderly
title_fullStr The signs of differentiation of chondrocytes in the formation of early cartilage lesions in the elderly
title_full_unstemmed The signs of differentiation of chondrocytes in the formation of early cartilage lesions in the elderly
title_sort signs of differentiation of chondrocytes in the formation of early cartilage lesions in the elderly
publisher IMA-PRESS LLC
series Научно-практическая ревматология
issn 1995-4484
1995-4492
publishDate 2011-02-01
description Objective. To study the relationship between the activity of collagen type II cleavage and the pattern of expression of the genes responsible for chondrocyte differentiation in the areas of intact cartilaginous tissue and in those of early focal cartilage lesions similar to those observed in elderly people with osteoarthrosis (OA). Material and methods. The distal femoral articular surface of the knee joint that articulated with the patella and had focal lesions was examined in the elderly. Collagen type II cleavage was estimated by enzyme immunoassay. Gene expression was determined with reverse-transcription polymerase chain reaction. Results. The activity of collagen type II cleavage was shown to be increased in the area of age-related OA-like cartilage lesions. This was accompanied by the high expression of collagenases of metalloproteinases (MMP) 1, 14 (MT1-MMP), aggrecanases - desintegrin and MMP with thrombospondin type 1 motif (ADAMTS) 5, the cytokines of interleukins (IL) 1α/β and tumor necrosis factor-α (TNF-α), as well as the genes associated with chondrocyte hypertrophy of type X collagen (C0L10A1), MMP 13 and 9, Indian hedgehog (Ihh) and cas-pase 3 in the immediate vicinity of a lesion area. At the same time, there was a high expression of growth factors associated with the proliferation phase of chondrocytes, namely: parathyroid hormone-related peptide (PTHrP), fibroblast growth factor-2 (FGF-2), transforming growth factor β1/2 (TGF-β1/2), as well as macromolecules of matrix of type II collagen (C0L2A1) and aggrecan in both the areas adjacent to the lesions and at a considerable distance from their center. However, these areas showed no higher collagen cleavage activity. Nether higher collagen cleavage, nor excess expression of the genes examined were observed in the absolutely intact cartilage areas. Conclusion. Our studies have indicated that the area of very early age-related OA-like focal cartilage lesions exhibits enhanced type II collagen cleavage that is attended by the expression of the genes associated with chondrocyte differentiation in the embryonic growth plate.
topic articular cartilage
chondrocyte differentiation
gene expression
focal lesions
url https://rsp.mediar-press.net/rsp/article/view/998
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