Cryptosporidium Infection in Patients with Gastroenteritis in Sari, Iran
Background: Cryptosporidiosis is a common coccidian parasite infection in patients with diarrhea that has worldwide distribution especially in developed countries. Therefore,the aim of this study was to determine the occurrence of Cryptosporidium infection in patients with gastroenteritis admitted t...
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Tehran University of Medical Sciences
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doaj-aa9ad56215054378ab58eb6b0ccf51802021-04-02T11:02:50ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2014-06-0192388Cryptosporidium Infection in Patients with Gastroenteritis in Sari, IranShirzad Gholami0Majid Khanmohammadi1Ehsan Ahmadpour2Abdol Sattar Pagheh3Sara Khadem Nakhjiri4Hamidreza Ramazannipour5Abbas Shahbazi6Molecular and Cell Biology Research Center , Mazandaran University of Medical Sciences, Iran.Department of Laboratory Sciences, Marand Branch, Islamic Azad University, Iran.Molecular and Cell Biology Research Center , Mazandaran University of Medical Sciences, Iran.Molecular and Cell Biology Research Center , Mazandaran University of Medical Sciences, Iran.Gorgan Faculty of Medicine, Golestan University of Medical Sciences, Iran.Faculty of Basic Sciences, University of Mazandaran, Iran.Tabriz Research Center of Infectious and Tropical Diseases, Tabriz University of Medical sciences, Iran.Background: Cryptosporidiosis is a common coccidian parasite infection in patients with diarrhea that has worldwide distribution especially in developed countries. Therefore,the aim of this study was to determine the occurrence of Cryptosporidium infection in patients with gastroenteritis admitted to hospitals of Mazandaran University of Medical Sciences by parasitological and molecular methods in Sari, Iran. Methods: Stool samples were collected from 348 patients with gastroenteritis admitted to the hospitals of Medical University in the Sari and Ghaemshahr cities in Mazandaran Province, Northern Iran in 2010-2011. Oocysts of Cryptosporidium identified using Formalin-Ether concentration method and stained by Aacid-fast staining (AFS)and Auramine phenol fluorescence (APF). Genomic DAN extracted from microscopically positive samples and nested PCR -RFLP by using SSU rRNA that identifies of the species of cryptosporidium. Results: In 348 patients with gastroenteritis, the most clinical symptoms were diarrhea, nausea, vomiting, dehydration, fever and weight loss. 2.3% (8 cases) of diarrheal samples tested by both microscopy and molecular methods were positive for the presence of cryptosporidium. Nested PCR products yielded unique bands of 846 bp, correspond to cryptosporidium. Species diagnosis carried out by digesting the secondary PCR product with SspI restriction enzyme, which noted 3 clearly bands of 449, 254, and 108 bp correspond to Cryptosporidium spp. Conclusion: The results of present study on Cryptosporidium spp. in this area can make a background data for control programs and further molecular analyses. Thus, further work needs to determine the origin of Cryptosporidium species in this area. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/409Cryptosporidium sppGastroenteritis PatientsNested PCR |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shirzad Gholami Majid Khanmohammadi Ehsan Ahmadpour Abdol Sattar Pagheh Sara Khadem Nakhjiri Hamidreza Ramazannipour Abbas Shahbazi |
spellingShingle |
Shirzad Gholami Majid Khanmohammadi Ehsan Ahmadpour Abdol Sattar Pagheh Sara Khadem Nakhjiri Hamidreza Ramazannipour Abbas Shahbazi Cryptosporidium Infection in Patients with Gastroenteritis in Sari, Iran Iranian Journal of Parasitology Cryptosporidium spp Gastroenteritis Patients Nested PCR |
author_facet |
Shirzad Gholami Majid Khanmohammadi Ehsan Ahmadpour Abdol Sattar Pagheh Sara Khadem Nakhjiri Hamidreza Ramazannipour Abbas Shahbazi |
author_sort |
Shirzad Gholami |
title |
Cryptosporidium Infection in Patients with Gastroenteritis in Sari, Iran |
title_short |
Cryptosporidium Infection in Patients with Gastroenteritis in Sari, Iran |
title_full |
Cryptosporidium Infection in Patients with Gastroenteritis in Sari, Iran |
title_fullStr |
Cryptosporidium Infection in Patients with Gastroenteritis in Sari, Iran |
title_full_unstemmed |
Cryptosporidium Infection in Patients with Gastroenteritis in Sari, Iran |
title_sort |
cryptosporidium infection in patients with gastroenteritis in sari, iran |
publisher |
Tehran University of Medical Sciences |
series |
Iranian Journal of Parasitology |
issn |
1735-7020 2008-238X |
publishDate |
2014-06-01 |
description |
Background: Cryptosporidiosis is a common coccidian parasite infection in patients with diarrhea that has worldwide distribution especially in developed countries. Therefore,the aim of this study was to determine the occurrence of Cryptosporidium infection in patients with gastroenteritis admitted to hospitals of Mazandaran University of Medical Sciences by parasitological and molecular methods in Sari, Iran.
Methods: Stool samples were collected from 348 patients with gastroenteritis admitted to the hospitals of Medical University in the Sari and Ghaemshahr cities in Mazandaran Province, Northern Iran in 2010-2011. Oocysts of Cryptosporidium identified using Formalin-Ether concentration method and stained by Aacid-fast staining (AFS)and Auramine phenol fluorescence (APF). Genomic DAN extracted from microscopically positive samples and nested PCR -RFLP by using SSU rRNA that identifies of the species of cryptosporidium.
Results: In 348 patients with gastroenteritis, the most clinical symptoms were diarrhea, nausea, vomiting, dehydration, fever and weight loss. 2.3% (8 cases) of diarrheal samples tested by both microscopy and molecular methods were positive for the presence of cryptosporidium. Nested PCR products yielded unique bands of 846 bp, correspond to cryptosporidium. Species diagnosis carried out by digesting the secondary PCR product with SspI restriction enzyme, which noted 3 clearly bands of 449, 254, and 108 bp correspond to Cryptosporidium spp.
Conclusion: The results of present study on Cryptosporidium spp. in this area can make a background data for control programs and further molecular analyses. Thus, further work needs to determine the origin of Cryptosporidium species in this area.
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topic |
Cryptosporidium spp Gastroenteritis Patients Nested PCR |
url |
https://ijpa.tums.ac.ir/index.php/ijpa/article/view/409 |
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