Genome editing of the SfABCC2 gene confers resistance to Cry1F toxin from Bacillus thuringiensis in Spodoptera frugiperda
ATP-binding cassette transporter C2 (ABCC2) is known to be a receptor for Bacillus thuringiensis (Bt) toxins in several lepidopteran insects. Mutations in the ABCC2 gene have been genetically linked to field-evolved resistance to the Cry1F toxin from Bt in Spodoptera frugiperda. Here we generated a...
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doaj-abb16e87184e4f5ab49f6a77564212fa2021-06-08T04:41:49ZengElsevierJournal of Integrative Agriculture2095-31192021-03-01203815820Genome editing of the SfABCC2 gene confers resistance to Cry1F toxin from Bacillus thuringiensis in Spodoptera frugiperdaMing-hui JIN0Jia-hui TAO1Qi LI2Ying CHENG3Xiao-xu SUN4Kong-ming WU5Yu-tao XIAO6Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, P.R.China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaAgricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, P.R.ChinaAgricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, P.R.ChinaAgricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, P.R.ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaAgricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, P.R.China; Correspondence XIAO Yu-taoATP-binding cassette transporter C2 (ABCC2) is known to be a receptor for Bacillus thuringiensis (Bt) toxins in several lepidopteran insects. Mutations in the ABCC2 gene have been genetically linked to field-evolved resistance to the Cry1F toxin from Bt in Spodoptera frugiperda. Here we generated a SfABCC2 knockout strain of S. frugiperda using the CRISPR/Cas9 system to provide further functional evidence of the role of this gene in susceptibility and resistance to Cry1F. Results from bioassays showed that the SfABCC2 knockout S. frugiperda strain displayed 118-fold resistance to Cry1F compared with the parental DH19 strain, but no resistance to Vip3A toxin from Bt. These results provide the first reverse genetic evidence for SfABCC2 as a functional receptor for Cry1F.http://www.sciencedirect.com/science/article/pii/S2095311919627723Spodoptera frugiperdaABCC2CRISPR/Cas9Bt receptorCry1F |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ming-hui JIN Jia-hui TAO Qi LI Ying CHENG Xiao-xu SUN Kong-ming WU Yu-tao XIAO |
spellingShingle |
Ming-hui JIN Jia-hui TAO Qi LI Ying CHENG Xiao-xu SUN Kong-ming WU Yu-tao XIAO Genome editing of the SfABCC2 gene confers resistance to Cry1F toxin from Bacillus thuringiensis in Spodoptera frugiperda Journal of Integrative Agriculture Spodoptera frugiperda ABCC2 CRISPR/Cas9 Bt receptor Cry1F |
author_facet |
Ming-hui JIN Jia-hui TAO Qi LI Ying CHENG Xiao-xu SUN Kong-ming WU Yu-tao XIAO |
author_sort |
Ming-hui JIN |
title |
Genome editing of the SfABCC2 gene confers resistance to Cry1F toxin from Bacillus thuringiensis in Spodoptera frugiperda |
title_short |
Genome editing of the SfABCC2 gene confers resistance to Cry1F toxin from Bacillus thuringiensis in Spodoptera frugiperda |
title_full |
Genome editing of the SfABCC2 gene confers resistance to Cry1F toxin from Bacillus thuringiensis in Spodoptera frugiperda |
title_fullStr |
Genome editing of the SfABCC2 gene confers resistance to Cry1F toxin from Bacillus thuringiensis in Spodoptera frugiperda |
title_full_unstemmed |
Genome editing of the SfABCC2 gene confers resistance to Cry1F toxin from Bacillus thuringiensis in Spodoptera frugiperda |
title_sort |
genome editing of the sfabcc2 gene confers resistance to cry1f toxin from bacillus thuringiensis in spodoptera frugiperda |
publisher |
Elsevier |
series |
Journal of Integrative Agriculture |
issn |
2095-3119 |
publishDate |
2021-03-01 |
description |
ATP-binding cassette transporter C2 (ABCC2) is known to be a receptor for Bacillus thuringiensis (Bt) toxins in several lepidopteran insects. Mutations in the ABCC2 gene have been genetically linked to field-evolved resistance to the Cry1F toxin from Bt in Spodoptera frugiperda. Here we generated a SfABCC2 knockout strain of S. frugiperda using the CRISPR/Cas9 system to provide further functional evidence of the role of this gene in susceptibility and resistance to Cry1F. Results from bioassays showed that the SfABCC2 knockout S. frugiperda strain displayed 118-fold resistance to Cry1F compared with the parental DH19 strain, but no resistance to Vip3A toxin from Bt. These results provide the first reverse genetic evidence for SfABCC2 as a functional receptor for Cry1F. |
topic |
Spodoptera frugiperda ABCC2 CRISPR/Cas9 Bt receptor Cry1F |
url |
http://www.sciencedirect.com/science/article/pii/S2095311919627723 |
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