Interferon-γ responses to Plasmodium falciparum vaccine candidate antigens decrease in the absence of malaria transmission
Background Malaria elimination campaigns are planned or active in many countries. The effects of malaria elimination on immune responses such as antigen-specific IFN- γ responses are not well characterized. Methods IFN- γ responses to the P. falciparum antigens circumsporozoite protein, liver stage...
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doaj-ac138d89c32b4c2bb0a30046042d8b572020-11-24T23:58:53ZengPeerJ Inc.PeerJ2167-83592017-01-015e285510.7717/peerj.2855Interferon-γ responses to Plasmodium falciparum vaccine candidate antigens decrease in the absence of malaria transmissionCyrus Ayieko0Bilha S. Ogola1Lyticia Ochola2Gideon A.M. Ngwena3George Ayodo4James S. Hodges5Gregory S. Noland6Chandy C. John7Department of Zoology, Maseno University, Maseno, KenyaDepartment of Biological Sciences, Masai Mara University, Narok, KenyaDepartment Biological Sciences, Kabianga University, Kericho, KenyaSchool of Medicine, Maseno University, Maseno, KenyaSchool of Health Sciences, Jaramogi Oginga Odinga University of Science and Technology, Bondo, KenyaSchool of Public Health, University of Minnesota, Minneapolis, MN, United StatesMedical School, University of Minnesota, Minneapolis, MN, United StatesMedical School, Indiana University, Indianapolis, IN, United StatesBackground Malaria elimination campaigns are planned or active in many countries. The effects of malaria elimination on immune responses such as antigen-specific IFN- γ responses are not well characterized. Methods IFN- γ responses to the P. falciparum antigens circumsporozoite protein, liver stage antigen-1, thrombospondin-related adhesive protein, apical membrane antigen-1, MB2, and merozoite surface protein-1 were tested by ELISA in 243 individuals in highland Kenya in April 2008, October 2008, and April 2009, after a one-year period of interrupted malaria transmission from April 2007 to March 2008. Results While one individual (0.4%) tested positive for P. falciparum by PCR inOctober 2008 and another two (0.9%) tested positive in April 2009, no clinical malaria cases were detected during weekly visits. Levels of IFN-γ to all antigens decreased significantly from April 2008 to April 2009 (all P < 0.001). Discussion Naturally acquired IFN- γ responses to P. falciparum antigensare short-lived in the absence of repeated P. falciparum infection. Even short periods of malaria interruption may significantly decrease IFN-γ responses to P. falciparum antigens.https://peerj.com/articles/2855.pdfPlasmodium falciparumInterferon gammaMalariaHighland Kenya |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cyrus Ayieko Bilha S. Ogola Lyticia Ochola Gideon A.M. Ngwena George Ayodo James S. Hodges Gregory S. Noland Chandy C. John |
spellingShingle |
Cyrus Ayieko Bilha S. Ogola Lyticia Ochola Gideon A.M. Ngwena George Ayodo James S. Hodges Gregory S. Noland Chandy C. John Interferon-γ responses to Plasmodium falciparum vaccine candidate antigens decrease in the absence of malaria transmission PeerJ Plasmodium falciparum Interferon gamma Malaria Highland Kenya |
author_facet |
Cyrus Ayieko Bilha S. Ogola Lyticia Ochola Gideon A.M. Ngwena George Ayodo James S. Hodges Gregory S. Noland Chandy C. John |
author_sort |
Cyrus Ayieko |
title |
Interferon-γ responses to Plasmodium falciparum vaccine candidate antigens decrease in the absence of malaria transmission |
title_short |
Interferon-γ responses to Plasmodium falciparum vaccine candidate antigens decrease in the absence of malaria transmission |
title_full |
Interferon-γ responses to Plasmodium falciparum vaccine candidate antigens decrease in the absence of malaria transmission |
title_fullStr |
Interferon-γ responses to Plasmodium falciparum vaccine candidate antigens decrease in the absence of malaria transmission |
title_full_unstemmed |
Interferon-γ responses to Plasmodium falciparum vaccine candidate antigens decrease in the absence of malaria transmission |
title_sort |
interferon-γ responses to plasmodium falciparum vaccine candidate antigens decrease in the absence of malaria transmission |
publisher |
PeerJ Inc. |
series |
PeerJ |
issn |
2167-8359 |
publishDate |
2017-01-01 |
description |
Background Malaria elimination campaigns are planned or active in many countries. The effects of malaria elimination on immune responses such as antigen-specific IFN- γ responses are not well characterized. Methods IFN- γ responses to the P. falciparum antigens circumsporozoite protein, liver stage antigen-1, thrombospondin-related adhesive protein, apical membrane antigen-1, MB2, and merozoite surface protein-1 were tested by ELISA in 243 individuals in highland Kenya in April 2008, October 2008, and April 2009, after a one-year period of interrupted malaria transmission from April 2007 to March 2008. Results While one individual (0.4%) tested positive for P. falciparum by PCR inOctober 2008 and another two (0.9%) tested positive in April 2009, no clinical malaria cases were detected during weekly visits. Levels of IFN-γ to all antigens decreased significantly from April 2008 to April 2009 (all P < 0.001). Discussion Naturally acquired IFN- γ responses to P. falciparum antigensare short-lived in the absence of repeated P. falciparum infection. Even short periods of malaria interruption may significantly decrease IFN-γ responses to P. falciparum antigens. |
topic |
Plasmodium falciparum Interferon gamma Malaria Highland Kenya |
url |
https://peerj.com/articles/2855.pdf |
work_keys_str_mv |
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