A SIMPLE AND ECONOMICAL PROCEDURE FOR THE PURIFICATION OF IMMUNOGLOBULIN Y FROM EGG YOLK BY T-GEL CHROMATOGRAPHY
The use of immunoglobulin Y (IgY) instead of polyclonal antibodies prepared in mammals has been reported recently. These antibodies are found to have better properties in terms of specificity and ease of large scale production. In this study, proteins from egg yolk were extracted with acid and chlor...
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Tehran University of Medical Sciences
2000-04-01
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doaj-ad1b4c99124c473ab2bd248e5654d9d32020-11-25T04:12:41ZengTehran University of Medical SciencesIranian Journal of Allergy, Asthma and Immunology1735-15021735-52492000-04-01129A SIMPLE AND ECONOMICAL PROCEDURE FOR THE PURIFICATION OF IMMUNOGLOBULIN Y FROM EGG YOLK BY T-GEL CHROMATOGRAPHY M. Pour-Amir0 M.J. Rasaee1 Qujeq2 GIIR. Asadikaram M. Frozeh Moqhadam3 The use of immunoglobulin Y (IgY) instead of polyclonal antibodies prepared in mammals has been reported recently. These antibodies are found to have better properties in terms of specificity and ease of large scale production. In this study, proteins from egg yolk were extracted with acid and chloroform. The result of cellulose acetate electrophoresis at pH 9 showed some extra bands which were further purified with sodium sulfate precipitation (20%). The purified band was observed in the y globulin region. SDS-PAGE eletrophoresis of the product showed impurities which were removed by T-gel chromatography. Following that only H-chainof immunoglobulin Y with a molecular weight of about 66 KD was observed. Using the above procedure, a yield of around 40.7 mg IgY / egg was obtained.https://ijaai.tums.ac.ir/index.php/ijaai/article/view/9IgYT-Gel Chromatography |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
M. Pour-Amir M.J. Rasaee Qujeq GIIR. Asadikaram M. Frozeh Moqhadam |
spellingShingle |
M. Pour-Amir M.J. Rasaee Qujeq GIIR. Asadikaram M. Frozeh Moqhadam A SIMPLE AND ECONOMICAL PROCEDURE FOR THE PURIFICATION OF IMMUNOGLOBULIN Y FROM EGG YOLK BY T-GEL CHROMATOGRAPHY Iranian Journal of Allergy, Asthma and Immunology IgY T-Gel Chromatography |
author_facet |
M. Pour-Amir M.J. Rasaee Qujeq GIIR. Asadikaram M. Frozeh Moqhadam |
author_sort |
M. Pour-Amir |
title |
A SIMPLE AND ECONOMICAL PROCEDURE FOR THE PURIFICATION OF IMMUNOGLOBULIN Y FROM EGG YOLK BY T-GEL CHROMATOGRAPHY |
title_short |
A SIMPLE AND ECONOMICAL PROCEDURE FOR THE PURIFICATION OF IMMUNOGLOBULIN Y FROM EGG YOLK BY T-GEL CHROMATOGRAPHY |
title_full |
A SIMPLE AND ECONOMICAL PROCEDURE FOR THE PURIFICATION OF IMMUNOGLOBULIN Y FROM EGG YOLK BY T-GEL CHROMATOGRAPHY |
title_fullStr |
A SIMPLE AND ECONOMICAL PROCEDURE FOR THE PURIFICATION OF IMMUNOGLOBULIN Y FROM EGG YOLK BY T-GEL CHROMATOGRAPHY |
title_full_unstemmed |
A SIMPLE AND ECONOMICAL PROCEDURE FOR THE PURIFICATION OF IMMUNOGLOBULIN Y FROM EGG YOLK BY T-GEL CHROMATOGRAPHY |
title_sort |
simple and economical procedure for the purification of immunoglobulin y from egg yolk by t-gel chromatography |
publisher |
Tehran University of Medical Sciences |
series |
Iranian Journal of Allergy, Asthma and Immunology |
issn |
1735-1502 1735-5249 |
publishDate |
2000-04-01 |
description |
The use of immunoglobulin Y (IgY) instead of polyclonal antibodies prepared in mammals has been reported recently. These antibodies are found to have better properties in terms of specificity and ease of large scale production. In this study, proteins from egg yolk were extracted with acid and chloroform. The result of cellulose acetate electrophoresis at pH 9 showed some extra bands which were further purified with sodium sulfate precipitation (20%). The purified band was observed in the y globulin region. SDS-PAGE eletrophoresis of the product showed impurities which were removed by T-gel chromatography. Following that only H-chainof immunoglobulin Y with a molecular weight of about 66 KD was observed. Using the above procedure, a yield of around 40.7 mg IgY / egg was obtained. |
topic |
IgY T-Gel Chromatography |
url |
https://ijaai.tums.ac.ir/index.php/ijaai/article/view/9 |
work_keys_str_mv |
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