Isolation of plant nuclei suitable for flow cytometry from species with extremely mucilaginous compounds: an example in the genus Viola L. (Violaceae)

Isolation of plant nuclei suitable for flow cytometry from species with extremely mucilaginous compounds: an example in the genus Viola L. (Violaceae)

Flow cytometry analysis has been widely applied in the determination of nuclear DNA content and ploidy level in many organisms. Despite being the most appropriate method for DNA content measurement, flow cytometry also presents some limitations. A fairly common, but little-studied problem is the eff...

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Bibliographic Details
Main Authors: Eduardo Cires, Candela Cuesta, María Ángeles Fernández Casado, Herminio S. Nava, Víctor M. Vázquez, José Antonio Fernández Prieto
Format: Article
Language:English
Published: Consejo Superior de Investigaciones Científicas 2011-12-01
Series:Anales del Jardín Botánico de Madrid
Subjects:
chromosome number
flow cytometry
genome size
mucilaginous compounds
nuclear dna content
ploidy levels
section melanium
section viola
Online Access:http://rjb.revistas.csic.es/index.php/rjb/article/view/362

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http://rjb.revistas.csic.es/index.php/rjb/article/view/362

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