NanoBRET: The Bright Future of Proximity-Based Assays

NanoBRET: The Bright Future of Proximity-Based Assays

Bioluminescence resonance energy transfer (BRET) is a biophysical technique used to monitor proximity within live cells. BRET exploits the naturally occurring phenomenon of dipole-dipole energy transfer from a donor enzyme (luciferase) to an acceptor fluorophore following enzyme-mediated oxidation o...

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Bibliographic Details
Main Authors: Natasha C. Dale, Elizabeth K. M. Johnstone, Carl W. White, Kevin D. G. Pfleger
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-03-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
BRET
NanoLuc
Nluc
NanoBRET
fluorophore
ligand binding
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2019.00056/full

Internet

https://www.frontiersin.org/article/10.3389/fbioe.2019.00056/full

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