Identification of an unknown person using Y-chromosome markers and mitochondrial dna typing
Background: The present article is concerned within the scope of Forensic Medicine, specifically Forensic Genetics. The case was taken care of in the Genetic-Molecular Laboratory of the Odessa Regional Bureau of Forensic-Medical Examinations, in Ukraine, during January and February of 2014. Objecti...
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Faculty of Medicine University of Baghdad
2015-04-01
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doaj-af528ad1c83b4d2ab90ecb6b1bb323522020-11-25T00:31:49ZengFaculty of Medicine University of Baghdadمجلة كلية الطب0041-94192410-80572015-04-0157110.32007/med.1936/jfacmedbagdad.v57i1.22Identification of an unknown person using Y-chromosome markers and mitochondrial dna typingRuslan G. Krivda0Aymen A. Jassim1Tatiana B. Fernandez Trokhimtchouk2Genetic-Molecular Laboratory of the Odessa Regional Bureau of Forensic-Medical ExaminationsDept. of Forensic Medicine of the Odessa National Medical UniversityDept. of Forensic Medicine of the Odessa National Medical University Background: The present article is concerned within the scope of Forensic Medicine, specifically Forensic Genetics. The case was taken care of in the Genetic-Molecular Laboratory of the Odessa Regional Bureau of Forensic-Medical Examinations, in Ukraine, during January and February of 2014. Objectives: The aim of our work was to identify an unknown person, using the techniques: Y-chromosome markers and mitochondrial DNA typing. Materials and methods: The materials available for our procedure were: pieces of tissue in paraffin blocks, saved from the corpse of the unknown person; blood from a living male subject, who claimed to be the grandfather, and from two females, allegedly the sisters. From all of them we extracted nuclear DNA (nDNA) and mitochondrial DNA (mtDNA) respectively, that was analyzed to compare and draw the proper conclusions. Results: The analyzed genetic materials confirmed the kinship. Conclusion: Using Y-chromosome markers we were able to establish the correspondence of the subject to his paternal line, and by mitochondrial DNA typing we could establish his relation to the proper maternal line. http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/324Forensic Genetics, genetic markers, Y-chromosome markers, mitochondrial DNA sequencing, identification of a corpse. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ruslan G. Krivda Aymen A. Jassim Tatiana B. Fernandez Trokhimtchouk |
spellingShingle |
Ruslan G. Krivda Aymen A. Jassim Tatiana B. Fernandez Trokhimtchouk Identification of an unknown person using Y-chromosome markers and mitochondrial dna typing مجلة كلية الطب Forensic Genetics, genetic markers, Y-chromosome markers, mitochondrial DNA sequencing, identification of a corpse. |
author_facet |
Ruslan G. Krivda Aymen A. Jassim Tatiana B. Fernandez Trokhimtchouk |
author_sort |
Ruslan G. Krivda |
title |
Identification of an unknown person using Y-chromosome markers and mitochondrial dna typing |
title_short |
Identification of an unknown person using Y-chromosome markers and mitochondrial dna typing |
title_full |
Identification of an unknown person using Y-chromosome markers and mitochondrial dna typing |
title_fullStr |
Identification of an unknown person using Y-chromosome markers and mitochondrial dna typing |
title_full_unstemmed |
Identification of an unknown person using Y-chromosome markers and mitochondrial dna typing |
title_sort |
identification of an unknown person using y-chromosome markers and mitochondrial dna typing |
publisher |
Faculty of Medicine University of Baghdad |
series |
مجلة كلية الطب |
issn |
0041-9419 2410-8057 |
publishDate |
2015-04-01 |
description |
Background: The present article is concerned within the scope of Forensic Medicine, specifically Forensic Genetics. The case was taken care of in the Genetic-Molecular Laboratory of the Odessa Regional Bureau of Forensic-Medical Examinations, in Ukraine, during January and February of 2014.
Objectives: The aim of our work was to identify an unknown person, using the techniques: Y-chromosome markers and mitochondrial DNA typing.
Materials and methods: The materials available for our procedure were: pieces of tissue in paraffin blocks, saved from the corpse of the unknown person; blood from a living male subject, who claimed to be the grandfather, and from two females, allegedly the sisters. From all of them we extracted nuclear DNA (nDNA) and mitochondrial DNA (mtDNA) respectively, that was analyzed to compare and draw the proper conclusions.
Results: The analyzed genetic materials confirmed the kinship.
Conclusion: Using Y-chromosome markers we were able to establish the correspondence of the subject to his paternal line, and by mitochondrial DNA typing we could establish his relation to the proper maternal line.
|
topic |
Forensic Genetics, genetic markers, Y-chromosome markers, mitochondrial DNA sequencing, identification of a corpse. |
url |
http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/324 |
work_keys_str_mv |
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