Dipteran Carboxymethyl Chitosan as an Inexhaustible Derivative with a Potential Antiproliferative Activity in Hepatocellular Carcinoma Cells

Traditional folk therapies indicate that insects have diverse medicinal potentials. However, the therapeutic application of insect chitosan and its derivatives has not been explored. To investigate the application of chitosan and its derivatives, the carboxymethyl derivative of chitosan (CM-Ch) was...

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Main Authors: Rana M. Abdel Rahman, Hedayat Abdel Ghaffar, Afrah F. Alkhuriji, Mahmoud I. Khalil, Noha Amaly, Attalla F. El-Kott, Ahmed S. Sultan
Format: Article
Language:English
Published: Hindawi Limited 2020-01-01
Series:Evidence-Based Complementary and Alternative Medicine
Online Access:http://dx.doi.org/10.1155/2020/4396305
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spelling doaj-b257db98337f47b68cb2c3d8f111761f2020-11-25T03:42:09ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882020-01-01202010.1155/2020/43963054396305Dipteran Carboxymethyl Chitosan as an Inexhaustible Derivative with a Potential Antiproliferative Activity in Hepatocellular Carcinoma CellsRana M. Abdel Rahman0Hedayat Abdel Ghaffar1Afrah F. Alkhuriji2Mahmoud I. Khalil3Noha Amaly4Attalla F. El-Kott5Ahmed S. Sultan6Zoology Department, Faculty of Science, Damanhour University, Damanhour, EgyptZoology Department, Faculty of Science, Alexandria University, Alexandria, EgyptDepartment of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Biological Sciences, Faculty of Science, Beirut Arab University, LebanonPolymeric Materials Research Department, Advanced Technology and New Materials Research Institute (ATNMRI), City of Scientific Research and Technological Applications (SRTA-City), Alexandria, EgyptZoology Department, Faculty of Science, Damanhour University, Damanhour, EgyptBiochemistry Department, Faculty of Science, Alexandria University, Alexandria, EgyptTraditional folk therapies indicate that insects have diverse medicinal potentials. However, the therapeutic application of insect chitosan and its derivatives has not been explored. To investigate the application of chitosan and its derivatives, the carboxymethyl derivative of chitosan (CM-Ch) was extracted from two dipteran larvae species, Chrysomya albiceps and Sarcophaga aegyptiaca. The degree of deacetylation (DD) and CM-Ch functional groups were validated using Fourier-transform infrared (FTIR) spectroscopy analysis and proton nuclear magnetic resonance spectroscopy (1H NMR), respectively. The molecular weight was estimated using MALDI-TOF MS analysis. The effect of CM-Ch on the morphology and proliferation of human liver HepG2 cancer cells was assessed. IC50 of CM-Ch induced significant growth-inhibitory effects in HepG2 cells. CM-Ch treatment altered the morphology of HepG2 in a dose-dependent manner and induced apoptosis in a caspase-dependent manner. CM-Ch treatment showed no signs of toxicity, and no alterations in liver and kidney biochemical markers were observed in albino rats. A CM-Ch derivative from commercial crustacean chitosan was used to assess the efficacy of the insect-derived CM-Ch. The data presented here introduce insect CM-Ch as a promising, inexhaustible, safe derivative of chitosan with antitumor potential in liver cancer. This is the first report highlighting the anticancer activity of insect CM-Ch in hepatocellular carcinoma cells.http://dx.doi.org/10.1155/2020/4396305
collection DOAJ
language English
format Article
sources DOAJ
author Rana M. Abdel Rahman
Hedayat Abdel Ghaffar
Afrah F. Alkhuriji
Mahmoud I. Khalil
Noha Amaly
Attalla F. El-Kott
Ahmed S. Sultan
spellingShingle Rana M. Abdel Rahman
Hedayat Abdel Ghaffar
Afrah F. Alkhuriji
Mahmoud I. Khalil
Noha Amaly
Attalla F. El-Kott
Ahmed S. Sultan
Dipteran Carboxymethyl Chitosan as an Inexhaustible Derivative with a Potential Antiproliferative Activity in Hepatocellular Carcinoma Cells
Evidence-Based Complementary and Alternative Medicine
author_facet Rana M. Abdel Rahman
Hedayat Abdel Ghaffar
Afrah F. Alkhuriji
Mahmoud I. Khalil
Noha Amaly
Attalla F. El-Kott
Ahmed S. Sultan
author_sort Rana M. Abdel Rahman
title Dipteran Carboxymethyl Chitosan as an Inexhaustible Derivative with a Potential Antiproliferative Activity in Hepatocellular Carcinoma Cells
title_short Dipteran Carboxymethyl Chitosan as an Inexhaustible Derivative with a Potential Antiproliferative Activity in Hepatocellular Carcinoma Cells
title_full Dipteran Carboxymethyl Chitosan as an Inexhaustible Derivative with a Potential Antiproliferative Activity in Hepatocellular Carcinoma Cells
title_fullStr Dipteran Carboxymethyl Chitosan as an Inexhaustible Derivative with a Potential Antiproliferative Activity in Hepatocellular Carcinoma Cells
title_full_unstemmed Dipteran Carboxymethyl Chitosan as an Inexhaustible Derivative with a Potential Antiproliferative Activity in Hepatocellular Carcinoma Cells
title_sort dipteran carboxymethyl chitosan as an inexhaustible derivative with a potential antiproliferative activity in hepatocellular carcinoma cells
publisher Hindawi Limited
series Evidence-Based Complementary and Alternative Medicine
issn 1741-427X
1741-4288
publishDate 2020-01-01
description Traditional folk therapies indicate that insects have diverse medicinal potentials. However, the therapeutic application of insect chitosan and its derivatives has not been explored. To investigate the application of chitosan and its derivatives, the carboxymethyl derivative of chitosan (CM-Ch) was extracted from two dipteran larvae species, Chrysomya albiceps and Sarcophaga aegyptiaca. The degree of deacetylation (DD) and CM-Ch functional groups were validated using Fourier-transform infrared (FTIR) spectroscopy analysis and proton nuclear magnetic resonance spectroscopy (1H NMR), respectively. The molecular weight was estimated using MALDI-TOF MS analysis. The effect of CM-Ch on the morphology and proliferation of human liver HepG2 cancer cells was assessed. IC50 of CM-Ch induced significant growth-inhibitory effects in HepG2 cells. CM-Ch treatment altered the morphology of HepG2 in a dose-dependent manner and induced apoptosis in a caspase-dependent manner. CM-Ch treatment showed no signs of toxicity, and no alterations in liver and kidney biochemical markers were observed in albino rats. A CM-Ch derivative from commercial crustacean chitosan was used to assess the efficacy of the insect-derived CM-Ch. The data presented here introduce insect CM-Ch as a promising, inexhaustible, safe derivative of chitosan with antitumor potential in liver cancer. This is the first report highlighting the anticancer activity of insect CM-Ch in hepatocellular carcinoma cells.
url http://dx.doi.org/10.1155/2020/4396305
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