| Summary: | The genus <i>Curcuma</i> is part of the Zingiberaceae family, and many <i>Curcuma </i>species have been used as traditional medicine and cosmetics in Thailand. To find new cosmeceutical ingredients, the in vitro anti-inflammatory, anti-oxidant, and cytotoxic activities of four <i>Curcuma</i> species as well as the isolation of compounds from the most active crude extract (<i>C. aromatica</i>) were investigated. The crude extract of <i>C. aromatica</i> showed 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity with an IC<sub>50</sub> value of 102.3 μg/mL. The cytotoxicity effect of <i>C. aeruginosa, C. comosa, C. aromatica, </i>and<i> C. longa</i> extracts assessed with the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay at 200 μg/mL were 12.1 2.9, 14.4 4.1, 28.6 4.1, and 46.9 8.6, respectively. <i>C. aeruginosa </i>and<i> C. comosa</i> presented apoptosis cells (57.7 3.1% and 32.6 2.2%, respectively) using the CytoTox-ONE™ assay. Different crude extracts or phytochemicals purified from<i> C. aromatica</i> were evaluated for their anti-inflammatory properties. The crude extract of <i>C. aromatica</i> showed the highest potential to inhibit NF-κB activity, followed by <i>C. aeruginosa, C. comosa, </i>and <i>C. longa</i>, respectively. Among the various purified phytochemicals curcumin, germacrone, curdione, zederone, and curcumenol significantly inhibited NF-κB activation in tumor necrosis factor (TNF) stimulated HaCaT keratinocytes. Of all compounds, curcumin was the most potent anti-inflammatory.
|
|---|
