Optimal Isolation Protocols for Examining and Interrogating Mononuclear Phagocytes From Human Intestinal Tissue

Optimal Isolation Protocols for Examining and Interrogating Mononuclear Phagocytes From Human Intestinal Tissue

The human intestine contains numerous mononuclear phagocytes (MNP), including subsets of conventional dendritic cells (cDC), macrophages (Mf) and monocytes, each playing their own unique role within the intestinal immune system and homeostasis. The ability to isolate and interrogate MNPs from fresh...

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Bibliographic Details
Main Authors: Chloe M. Doyle, Erica E. Vine, Kirstie M. Bertram, Heeva Baharlou, Jake W. Rhodes, Suat Dervish, Martijn P. Gosselink, Angelina Di Re, Geoffrey P. Collins, Faizur Reza, James W. T. Toh, Nimalan Pathma-Nathan, Golo Ahlenstiel, Grahame Ctercteko, Anthony L. Cunningham, Andrew N. Harman, Scott N. Byrne
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-09-01
Series:Frontiers in Immunology
Subjects:
dendritic cells (DC)
flow cytometry
human tissue
intestine
enzymatic digestion
macrophage – cell
Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2021.727952/full

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