An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol

We report on the aptadetection of chloramphenicol (CAP) using electrochemical impedance spectroscopy. The detection principle is based on the changes of the interfacial properties of the electrode after the interaction of the ssDNA aptamers with the target molecules. The electrode surface is partial...

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Main Authors:	Sanaz Pilehvar, Tarryn Dierckx, Ronny Blust, Tom Breugelmans, Karolien De Wael
Format:	Article
Language:	English
Published:	MDPI AG 2014-07-01
Series:	Sensors
Subjects:	aptasensor electrochemical impedance spectroscopy chloramphenicol specific recognition
Online Access:	http://www.mdpi.com/1424-8220/14/7/12059

id	doaj-b6823fe34ed44ee98cbb3cc299e2c0a0
record_format	Article
spelling	doaj-b6823fe34ed44ee98cbb3cc299e2c0a02020-11-24T20:44:36ZengMDPI AGSensors1424-82202014-07-01147120591206910.3390/s140712059s140712059An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as ChloramphenicolSanaz Pilehvar0Tarryn Dierckx1Ronny Blust2Tom Breugelmans3Karolien De Wael4AXES Research Group, Department of Chemistry, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, BelgiumART Research Group, Faculty of Applied Engineering, University of Antwerp, Salesianenlaan 90, 2000 Antwerp, BelgiumSphere Research Group, Department of Biology, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, BelgiumART Research Group, Faculty of Applied Engineering, University of Antwerp, Salesianenlaan 90, 2000 Antwerp, BelgiumAXES Research Group, Department of Chemistry, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, BelgiumWe report on the aptadetection of chloramphenicol (CAP) using electrochemical impedance spectroscopy. The detection principle is based on the changes of the interfacial properties of the electrode after the interaction of the ssDNA aptamers with the target molecules. The electrode surface is partially blocked due to the formation of the aptamer-CAP complex, resulting in an increase of the interfacial electron-transfer resistance of the redox probe detected by electrochemical impedance spectroscopy or cyclic voltammetry. We observed that the ratio of polarization resistance had a linear relationship with the concentrations of CAP in the range of 1.76–127 nM, and a detection limit of 1.76 nM was obtained. The covalent binding of CAP-aptamer on the electrode surface combined with the unique properties of aptamers and impedimetric transduction leads to the development of a stable and sensitive electrochemical aptasensor for CAP.http://www.mdpi.com/1424-8220/14/7/12059aptasensorelectrochemical impedance spectroscopychloramphenicolspecific recognition
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Sanaz Pilehvar Tarryn Dierckx Ronny Blust Tom Breugelmans Karolien De Wael
spellingShingle	Sanaz Pilehvar Tarryn Dierckx Ronny Blust Tom Breugelmans Karolien De Wael An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol Sensors aptasensor electrochemical impedance spectroscopy chloramphenicol specific recognition
author_facet	Sanaz Pilehvar Tarryn Dierckx Ronny Blust Tom Breugelmans Karolien De Wael
author_sort	Sanaz Pilehvar
title	An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol
title_short	An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol
title_full	An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol
title_fullStr	An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol
title_full_unstemmed	An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol
title_sort	electrochemical impedimetric aptasensing platform for sensitive and selective detection of small molecules such as chloramphenicol
publisher	MDPI AG
series	Sensors
issn	1424-8220
publishDate	2014-07-01
description	We report on the aptadetection of chloramphenicol (CAP) using electrochemical impedance spectroscopy. The detection principle is based on the changes of the interfacial properties of the electrode after the interaction of the ssDNA aptamers with the target molecules. The electrode surface is partially blocked due to the formation of the aptamer-CAP complex, resulting in an increase of the interfacial electron-transfer resistance of the redox probe detected by electrochemical impedance spectroscopy or cyclic voltammetry. We observed that the ratio of polarization resistance had a linear relationship with the concentrations of CAP in the range of 1.76–127 nM, and a detection limit of 1.76 nM was obtained. The covalent binding of CAP-aptamer on the electrode surface combined with the unique properties of aptamers and impedimetric transduction leads to the development of a stable and sensitive electrochemical aptasensor for CAP.
topic	aptasensor electrochemical impedance spectroscopy chloramphenicol specific recognition
url	http://www.mdpi.com/1424-8220/14/7/12059
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An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol

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