Zika Virus-Induction of the Suppressor of Cytokine Signaling 1/3 Contributes to the Modulation of Viral Replication
Zika virus (ZIKV) is a mosquito-borne flavivirus that has emerged and caused global outbreaks since 2007. Although ZIKV proteins have been shown to suppress early anti-viral innate immune responses, little is known about the exact mechanisms. This study demonstrates that infection with either the Af...
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doaj-b6c10da15e00428991b45bc0c5ca06172020-11-25T02:15:56ZengMDPI AGPathogens2076-08172020-02-019316310.3390/pathogens9030163pathogens9030163Zika Virus-Induction of the Suppressor of Cytokine Signaling 1/3 Contributes to the Modulation of Viral ReplicationRak-Kyun Seong0Jae Kyung Lee1Ok Sarah Shin2Bk21 PLUS Program, Department of Biomedical Sciences, College of Medicine, Korea University Guro Hospital, Seoul 02841, KoreaBk21 PLUS Program, Department of Biomedical Sciences, College of Medicine, Korea University Guro Hospital, Seoul 02841, KoreaBk21 PLUS Program, Department of Biomedical Sciences, College of Medicine, Korea University Guro Hospital, Seoul 02841, KoreaZika virus (ZIKV) is a mosquito-borne flavivirus that has emerged and caused global outbreaks since 2007. Although ZIKV proteins have been shown to suppress early anti-viral innate immune responses, little is known about the exact mechanisms. This study demonstrates that infection with either the African or Asian lineage of ZIKV leads to a modulated expression of suppressor of cytokine signaling (SOCS) genes encoding SOCS1 and SOCS3 in the following cell models: A549 human lung adenocarcinoma cells; JAr human choriocarcinoma cells; human neural progenitor cells. Studies of viral gene expression in response to SOCS1 or SOCS3 demonstrated that the knockdown of these SOCS proteins inhibited viral NS5 or ZIKV RNA expression, whereas overexpression resulted in an increased expression. Moreover, the overexpression of SOCS1 or SOCS3 inhibited the retinoic acid-inducible gene-I-like receptor-mediated activation of both type I and III interferon pathways. These results imply that SOCS upregulation following ZIKV infection modulates viral replication, possibly via the regulation of anti-viral innate immune responses.https://www.mdpi.com/2076-0817/9/3/163suppressor of cytokine signalingzika virusinterferonantiviral |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rak-Kyun Seong Jae Kyung Lee Ok Sarah Shin |
spellingShingle |
Rak-Kyun Seong Jae Kyung Lee Ok Sarah Shin Zika Virus-Induction of the Suppressor of Cytokine Signaling 1/3 Contributes to the Modulation of Viral Replication Pathogens suppressor of cytokine signaling zika virus interferon antiviral |
author_facet |
Rak-Kyun Seong Jae Kyung Lee Ok Sarah Shin |
author_sort |
Rak-Kyun Seong |
title |
Zika Virus-Induction of the Suppressor of Cytokine Signaling 1/3 Contributes to the Modulation of Viral Replication |
title_short |
Zika Virus-Induction of the Suppressor of Cytokine Signaling 1/3 Contributes to the Modulation of Viral Replication |
title_full |
Zika Virus-Induction of the Suppressor of Cytokine Signaling 1/3 Contributes to the Modulation of Viral Replication |
title_fullStr |
Zika Virus-Induction of the Suppressor of Cytokine Signaling 1/3 Contributes to the Modulation of Viral Replication |
title_full_unstemmed |
Zika Virus-Induction of the Suppressor of Cytokine Signaling 1/3 Contributes to the Modulation of Viral Replication |
title_sort |
zika virus-induction of the suppressor of cytokine signaling 1/3 contributes to the modulation of viral replication |
publisher |
MDPI AG |
series |
Pathogens |
issn |
2076-0817 |
publishDate |
2020-02-01 |
description |
Zika virus (ZIKV) is a mosquito-borne flavivirus that has emerged and caused global outbreaks since 2007. Although ZIKV proteins have been shown to suppress early anti-viral innate immune responses, little is known about the exact mechanisms. This study demonstrates that infection with either the African or Asian lineage of ZIKV leads to a modulated expression of suppressor of cytokine signaling (SOCS) genes encoding SOCS1 and SOCS3 in the following cell models: A549 human lung adenocarcinoma cells; JAr human choriocarcinoma cells; human neural progenitor cells. Studies of viral gene expression in response to SOCS1 or SOCS3 demonstrated that the knockdown of these SOCS proteins inhibited viral NS5 or ZIKV RNA expression, whereas overexpression resulted in an increased expression. Moreover, the overexpression of SOCS1 or SOCS3 inhibited the retinoic acid-inducible gene-I-like receptor-mediated activation of both type I and III interferon pathways. These results imply that SOCS upregulation following ZIKV infection modulates viral replication, possibly via the regulation of anti-viral innate immune responses. |
topic |
suppressor of cytokine signaling zika virus interferon antiviral |
url |
https://www.mdpi.com/2076-0817/9/3/163 |
work_keys_str_mv |
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