Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses

Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses

Clinical manifestations of Zika virus, chikungunya virus, and dengue virus infections can be similar. To improve virus detection, streamline molecular workflow, and decrease test costs, we developed and evaluated a multiplex real-time reverse transcription PCR for these viruses.

Bibliographic Details
Main Authors: Jesse J. Waggoner, Lionel Gresh, Alisha Mohamed-Hadley, Gabriela Ballesteros, Maria Jose Vargas Davila, Yolanda Tellez, Malaya K. Sahoo, Angel Balmaseda, Eva Harris, Benjamin A. Pinsky
Format: Article
Language:English
Published: Centers for Disease Control and Prevention 2016-07-01
Series:Emerging Infectious Diseases
Subjects:
dengue virus
chikungunya virus
Zika virus
multiplex molecular diagnostics
real-time RT-PCR
viruses
Online Access:https://wwwnc.cdc.gov/eid/article/22/7/16-0326_article

Internet

https://wwwnc.cdc.gov/eid/article/22/7/16-0326_article

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