Prevalence of Campylobacter jejuni in Diarrhea samples in Gorgan ,East north of Iran
Abstract Background and Objectives: Diarrhea is one of the most common diseases in the world. Campylobacter jejuni is one of the prevalent agents of bacterial diarrhea in most of developing countries. It is usually ignored in routine laboratory test in our country, because it has a difficult investi...
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Golestan University of Medical Sciences
2008-10-01
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doaj-b750c735cc7548838c63bc8c46628e682021-09-01T13:20:26ZengGolestan University of Medical SciencesMedical Laboratory Journal2538-44492008-10-012200Prevalence of Campylobacter jejuni in Diarrhea samples in Gorgan ,East north of IranN Ziaei0N Amir Mozafari1H Kouhsari2A Moradi3A Tabarai4T Dadgar5S Livani6M Arab Ahmadi7 Ms in Microbiology,Lahijan Azad Associate professor in Molecular Biology, Abstract Background and Objectives: Diarrhea is one of the most common diseases in the world. Campylobacter jejuni is one of the prevalent agents of bacterial diarrhea in most of developing countries. It is usually ignored in routine laboratory test in our country, because it has a difficult investigation method. This article aims to determine the prevalence of Campylobacter jejuni, in diarrhea samples in Gorgan City (East north of Iran) by PCR Method. Material and Methods: This descriptive study was performed on 455 diarrheal samples during one year (2005-06).255 out of them were cultured on Preston media (Himedia co.) on 42°c. DNA Extracted by phenol cholorophorm method was directly carried out on stool samples.16srDNA hipo and asp primers for detection of Campylobacter genus, C.jejuni and C.coli species were used, respectively. In addition, universal primer of 16srDNA was used for control of PCR method. Results: no sample was positive for Campylobacter in culture .only three samples were positive for Campylobacter genus and C.jejuni specific primer but none of them were positive for C.coli .99 samples were positive by universal primer of 16srDNA . Conclusion: The results indicate that C.jejuni isnchr('39')t a prevalent agent in diarrhea in our region. Key words: Campylobacter jejuni -Gorgan- Diarrheahttp://mlj.goums.ac.ir/article-1-45-en.htmlkey words: campylobacter jejuni -gorgan- diarrhea |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
N Ziaei N Amir Mozafari H Kouhsari A Moradi A Tabarai T Dadgar S Livani M Arab Ahmadi |
spellingShingle |
N Ziaei N Amir Mozafari H Kouhsari A Moradi A Tabarai T Dadgar S Livani M Arab Ahmadi Prevalence of Campylobacter jejuni in Diarrhea samples in Gorgan ,East north of Iran Medical Laboratory Journal key words: campylobacter jejuni -gorgan- diarrhea |
author_facet |
N Ziaei N Amir Mozafari H Kouhsari A Moradi A Tabarai T Dadgar S Livani M Arab Ahmadi |
author_sort |
N Ziaei |
title |
Prevalence of Campylobacter jejuni in Diarrhea samples in Gorgan ,East north of Iran |
title_short |
Prevalence of Campylobacter jejuni in Diarrhea samples in Gorgan ,East north of Iran |
title_full |
Prevalence of Campylobacter jejuni in Diarrhea samples in Gorgan ,East north of Iran |
title_fullStr |
Prevalence of Campylobacter jejuni in Diarrhea samples in Gorgan ,East north of Iran |
title_full_unstemmed |
Prevalence of Campylobacter jejuni in Diarrhea samples in Gorgan ,East north of Iran |
title_sort |
prevalence of campylobacter jejuni in diarrhea samples in gorgan ,east north of iran |
publisher |
Golestan University of Medical Sciences |
series |
Medical Laboratory Journal |
issn |
2538-4449 |
publishDate |
2008-10-01 |
description |
Abstract Background and Objectives: Diarrhea is one of the most common diseases in the world. Campylobacter jejuni is one of the prevalent agents of bacterial diarrhea in most of developing countries. It is usually ignored in routine laboratory test in our country, because it has a difficult investigation method. This article aims to determine the prevalence of Campylobacter jejuni, in diarrhea samples in Gorgan City (East north of Iran) by PCR Method. Material and Methods: This descriptive study was performed on 455 diarrheal samples during one year (2005-06).255 out of them were cultured on Preston media (Himedia co.) on 42°c. DNA Extracted by phenol cholorophorm method was directly carried out on stool samples.16srDNA hipo and asp primers for detection of Campylobacter genus, C.jejuni and C.coli species were used, respectively. In addition, universal primer of 16srDNA was used for control of PCR method. Results: no sample was positive for Campylobacter in culture .only three samples were positive for Campylobacter genus and C.jejuni specific primer but none of them were positive for C.coli .99 samples were positive by universal primer of 16srDNA . Conclusion: The results indicate that C.jejuni isnchr('39')t a prevalent agent in diarrhea in our region. Key words: Campylobacter jejuni -Gorgan- Diarrhea |
topic |
key words: campylobacter jejuni -gorgan- diarrhea |
url |
http://mlj.goums.ac.ir/article-1-45-en.html |
work_keys_str_mv |
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