Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells

Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells

Summary: Pulmonary neuroendocrine cells (PNECs) are sensory cells within the lung airway epithelia. Here, we provide a detailed protocol for generating induced PNECs (iPNECs) from human induced pluripotent stem cells (iPSCs). The cellular and molecular profile of iPNECs resembles primary human PNECs...

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Main Authors: Pooja Hor, Justin K. Ichida, Zea Borok, Amy L. Ryan
Format: Article
Language:English
Published: Elsevier 2020-09-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720300551
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spelling doaj-b7832cccd3e343658b9fd64571eb1abe2020-11-25T04:06:45ZengElsevierSTAR Protocols2666-16672020-09-0112100068Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine CellsPooja Hor0Justin K. Ichida1Zea Borok2Amy L. Ryan3Hastings Center for Pulmonary Research and Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA; Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USADepartment of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USAHastings Center for Pulmonary Research and Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA; Department of Biochemistry and Molecular Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA; Norris Comprehensive Center, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA; Corresponding authorHastings Center for Pulmonary Research and Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA; Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA; Corresponding authorSummary: Pulmonary neuroendocrine cells (PNECs) are sensory cells within the lung airway epithelia. Here, we provide a detailed protocol for generating induced PNECs (iPNECs) from human induced pluripotent stem cells (iPSCs). The cellular and molecular profile of iPNECs resembles primary human PNECs. Primary human PNECs are exceedingly rare, comprising only 1% of the adult lung. Therefore, a self-renewing source of patient-specific iPNECs facilitates the creation of reproducible human cellular models to study lung diseases characterized by PNEC dysfunction.For complete details on the use and execution of this protocol, please refer to Hor et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166720300551
collection DOAJ
language English
format Article
sources DOAJ
author Pooja Hor
Justin K. Ichida
Zea Borok
Amy L. Ryan
spellingShingle Pooja Hor
Justin K. Ichida
Zea Borok
Amy L. Ryan
Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells
STAR Protocols
author_facet Pooja Hor
Justin K. Ichida
Zea Borok
Amy L. Ryan
author_sort Pooja Hor
title Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells
title_short Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells
title_full Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells
title_fullStr Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells
title_full_unstemmed Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells
title_sort protocol for differentiation of human ipscs into pulmonary neuroendocrine cells
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2020-09-01
description Summary: Pulmonary neuroendocrine cells (PNECs) are sensory cells within the lung airway epithelia. Here, we provide a detailed protocol for generating induced PNECs (iPNECs) from human induced pluripotent stem cells (iPSCs). The cellular and molecular profile of iPNECs resembles primary human PNECs. Primary human PNECs are exceedingly rare, comprising only 1% of the adult lung. Therefore, a self-renewing source of patient-specific iPNECs facilitates the creation of reproducible human cellular models to study lung diseases characterized by PNEC dysfunction.For complete details on the use and execution of this protocol, please refer to Hor et al. (2020).
url http://www.sciencedirect.com/science/article/pii/S2666166720300551
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AT zeaborok protocolfordifferentiationofhumanipscsintopulmonaryneuroendocrinecells
AT amylryan protocolfordifferentiationofhumanipscsintopulmonaryneuroendocrinecells
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