Evaluation of Internal Reference Genes for Quantitative Expression Analysis by Real-Time PCR in Ovine Whole Blood

Evaluation of Internal Reference Genes for Quantitative Expression Analysis by Real-Time PCR in Ovine Whole Blood

The use of reference genes is commonly accepted as the most reliable approach to normalize qRT-PCR and to reduce possible errors in the quantification of gene expression. The most suitable reference genes in sheep have been identified for a restricted range of tissues, but no specific data on whole...

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Bibliographic Details
Main Authors: Pier Luigi Acutis, Maria Caramelli, Antonio Quasso, Dario Ariello, Claudio Bellino, Maria Grazia Maniaci, Paola Modesto, Chiara Campanella, Simone Bertuzzi, Simone Peletto
Format: Article
Language:English
Published: MDPI AG 2011-11-01
Series:International Journal of Molecular Sciences
Subjects:
reference genes
Ovis aries
whole blood
real-time qPCR
normalization
Online Access:http://www.mdpi.com/1422-0067/12/11/7732/

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http://www.mdpi.com/1422-0067/12/11/7732/

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