Comparing Properties of Variable Pore-Sized 3D-Printed PLA Membrane with Conventional PLA Membrane for Guided Bone/Tissue Regeneration
The aim of this study was to fabricate bioresorbable polylactide (PLA) membranes by 3D printing and compare their properties to those of the membranes fabricated by the conventional method and compare the effect of different pore sizes on the properties of the 3D-printed membranes. PLA membranes wit...
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doaj-b96097d89dd847d3ad19be2e49b1170e2020-11-25T01:14:53ZengMDPI AGMaterials1996-19442019-05-011210171810.3390/ma12101718ma12101718Comparing Properties of Variable Pore-Sized 3D-Printed PLA Membrane with Conventional PLA Membrane for Guided Bone/Tissue RegenerationHao Yang Zhang0Heng Bo Jiang1Jeong-Hyun Ryu2Hyojin Kang3Kwang-Mahn Kim4Jae-Sung Kwon5Department and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, Seoul 03722, KoreaThe CONVERSATIONALIST Club, School of Stomatology, Shandong First Medical University & Shandong Academy of Medical Sciences, Tai’an 271016, Shandong, ChinaDepartment and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, Seoul 03722, KoreaDepartment and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, Seoul 03722, KoreaDepartment and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, Seoul 03722, KoreaDepartment and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, Seoul 03722, KoreaThe aim of this study was to fabricate bioresorbable polylactide (PLA) membranes by 3D printing and compare their properties to those of the membranes fabricated by the conventional method and compare the effect of different pore sizes on the properties of the 3D-printed membranes. PLA membranes with three different pore sizes (large pore-479 μm, small pore-273 μm, and no pore) were 3D printed, and membranes fabricated using the conventional solvent casting method were used as the control group. Scanning electron microscopy (SEM) and micro-computed tomography (µ-CT) were taken to observe the morphology and obtain the porosity of the four groups. A tensile test was performed to compare the tensile strength, elastic modulus, and elongation at break of the membranes. Preosteoblast cells were cultured on the membranes for 1, 3 and 7 days, followed by a WST assay and SEM, to examine the cell proliferation on different groups. As a result, the 3D-printed membranes showed superior mechanical properties to those of the solvent cast membranes, and the 3D-printed membranes exhibited different advantageous mechanical properties depending on the different pore sizes. The various fabrication methods and pore sizes did not have significantly different effects on cell growth. It is proven that 3D printing is a promising method for the fabrication of customized barrier membranes used in GBR/GTR.https://www.mdpi.com/1996-1944/12/10/17183D printingpolylactideguided bone regenerationguided tissue regenerationartificial membranesbiomaterials |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hao Yang Zhang Heng Bo Jiang Jeong-Hyun Ryu Hyojin Kang Kwang-Mahn Kim Jae-Sung Kwon |
spellingShingle |
Hao Yang Zhang Heng Bo Jiang Jeong-Hyun Ryu Hyojin Kang Kwang-Mahn Kim Jae-Sung Kwon Comparing Properties of Variable Pore-Sized 3D-Printed PLA Membrane with Conventional PLA Membrane for Guided Bone/Tissue Regeneration Materials 3D printing polylactide guided bone regeneration guided tissue regeneration artificial membranes biomaterials |
author_facet |
Hao Yang Zhang Heng Bo Jiang Jeong-Hyun Ryu Hyojin Kang Kwang-Mahn Kim Jae-Sung Kwon |
author_sort |
Hao Yang Zhang |
title |
Comparing Properties of Variable Pore-Sized 3D-Printed PLA Membrane with Conventional PLA Membrane for Guided Bone/Tissue Regeneration |
title_short |
Comparing Properties of Variable Pore-Sized 3D-Printed PLA Membrane with Conventional PLA Membrane for Guided Bone/Tissue Regeneration |
title_full |
Comparing Properties of Variable Pore-Sized 3D-Printed PLA Membrane with Conventional PLA Membrane for Guided Bone/Tissue Regeneration |
title_fullStr |
Comparing Properties of Variable Pore-Sized 3D-Printed PLA Membrane with Conventional PLA Membrane for Guided Bone/Tissue Regeneration |
title_full_unstemmed |
Comparing Properties of Variable Pore-Sized 3D-Printed PLA Membrane with Conventional PLA Membrane for Guided Bone/Tissue Regeneration |
title_sort |
comparing properties of variable pore-sized 3d-printed pla membrane with conventional pla membrane for guided bone/tissue regeneration |
publisher |
MDPI AG |
series |
Materials |
issn |
1996-1944 |
publishDate |
2019-05-01 |
description |
The aim of this study was to fabricate bioresorbable polylactide (PLA) membranes by 3D printing and compare their properties to those of the membranes fabricated by the conventional method and compare the effect of different pore sizes on the properties of the 3D-printed membranes. PLA membranes with three different pore sizes (large pore-479 μm, small pore-273 μm, and no pore) were 3D printed, and membranes fabricated using the conventional solvent casting method were used as the control group. Scanning electron microscopy (SEM) and micro-computed tomography (µ-CT) were taken to observe the morphology and obtain the porosity of the four groups. A tensile test was performed to compare the tensile strength, elastic modulus, and elongation at break of the membranes. Preosteoblast cells were cultured on the membranes for 1, 3 and 7 days, followed by a WST assay and SEM, to examine the cell proliferation on different groups. As a result, the 3D-printed membranes showed superior mechanical properties to those of the solvent cast membranes, and the 3D-printed membranes exhibited different advantageous mechanical properties depending on the different pore sizes. The various fabrication methods and pore sizes did not have significantly different effects on cell growth. It is proven that 3D printing is a promising method for the fabrication of customized barrier membranes used in GBR/GTR. |
topic |
3D printing polylactide guided bone regeneration guided tissue regeneration artificial membranes biomaterials |
url |
https://www.mdpi.com/1996-1944/12/10/1718 |
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